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Mature and immature synaptosomal membranes have a different lipid composition

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Abstract

Subfractionation of the optic tectum in chick embryos results in the isolation of two fractions enriched in synaptosomes (fraction A and fraction B). In chicks after hatching, this fractionation results in the isolation of a single synaptosomal fraction (fraction B) and of a fraction enriched in myelin membranes devoid of synaptosomes (fraction A). The lipid composition of synaptosomal fractions (A and B) and corresponding synaptosomal plasma membranes has been analyzed and compared to the lipid composition of similar fractions isolated from 2–3 day-old chicks. The phospholipid composition of fraction A in embryos was mainly represented by phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The PE content was significantly lower than that of PC, which accounted for by approximately 50%. Sphingomyelin (SP) and phosphatidylinositol (PI) accounted for by only 6% of the total membrane phopsholipids. Fraction A isolated from the young chicks showed many significant changes. PC accounted for by approximately 40% and PE made up 35%. The amount of phosphatidylserine (PS) and SP increased. These data parallel our previous morphological observations, which showed that fraction A contains immature synaptosomes in embryos but myelin membranes and no synaptosomes in the young chicks. Fraction B has been shown to contain synaptosomes at all stages considered. It possessed in embryos a lipid composition similar to fraction A, except that PC content was higher in young embryos. The analyses on membrane fractions confirmed these results. On the contrary, this fraction showed many significant changes after hatching. The content of PC was significantly reduced, PE/PC ratio was significantly increased as well as ethanolamine plasmalogen (PLE) content. The percentage of PS, PI and SP were increased. The composition of fatty acids of the total fraction of phospholipids was also examined. The results parallel the observations on phospholipid classes.

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Authors and Affiliations

  1. Institute of Human Anatomy, School of Medicine, University of Cagliari, Via Porcell 2, 09100, Cagliari, Italy

    F. Gremo & S. Porru

  2. Institute of Biochemistry School of Medicine, University of Perugia, Perugia, Italy

    G. E. De Medio & G. Trovarelli

  3. Institute of General Pathology School of Medicine, University of Cagliari, Italy

    S. Dessi

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  1. F. Gremo
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  4. S. Dessi
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  5. S. Porru
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Gremo, F., De Medio, G.E., Trovarelli, G. et al. Mature and immature synaptosomal membranes have a different lipid composition. Neurochem Res 10, 133–144 (1985). https://doi.org/10.1007/BF00964778

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