Skip to main content
SpringerLink
Log in

Continuous-flow enrichment of a strain ofErwinia carotovora having specificity for highly methylated pectin

  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

A pectinolytic bacterium was isolated from a mixed microbial population by means of a chemostat enrichment procedure. The bacterium, which was identified asErwinia carotovora, grew only on highly methylated pectin and produced a pectin lysase which released unsaturated monomer and dimer from 71% esterified citrus pectin. The pectin lyase was inducible only by pectins having a high methyl content and in pectin-limited chemostats its synthesis passed through a maximum at a dilution rate close to 0.04h-1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Buchanan, R. E., Gibbons, N. E.: Bergey's manual of determinative bacteriology, 8th ed. Baltimore: Williams & Wilkins 1974

    Google Scholar 

  • Carter, B. L. A., Bull, A. T.: Studies of fungal growth and intermediary carbon metabolism under steady and non-steady state conditions. Biotechnol. Bioeng.11, 785–804 (1969)

    Google Scholar 

  • Clarke, P. H., Houldsworth, M. A., Lilly, M. D.: Catabolite repression and induction of amidase synthesis ofPseudomonas aeruginosa 8602 in continuous culture. J. Gen. Microbiol.51, 225–234 (1968)

    PubMed  Google Scholar 

  • Cowan, S. T., Steel, K. J.: Manual for the identification of medical bacteria. Cambridge: Cambridge University Press 1970

    Google Scholar 

  • Fogarty, W. M., Ward, O. P.: Pectinases and pectic polysaccharides. Prog. Ind. Microbiol.13, 59–119 (1974)

    Google Scholar 

  • Fuchs, A.: The trans-eliminative breakdown of Na-polygalacturonate byPseudomonas fluorescens. Antonie van Leeuwenhoek31, 323–340 (1965)

    PubMed  Google Scholar 

  • Hasegawa, S., Nagel, C. W.: The characterization of an α, β unsaturated digalacturonic acid. J. Biol. Chem.237, 619–621 (1962)

    PubMed  Google Scholar 

  • Henderson, C., Hobson, P. N., Summers, R.: The production of amylase, protease and lipolytic enzymes by two species of anaerobic rumen bacteria. In: Proceedings of the Fourth Symposium on Continuous Culture of Microorganisms, pp. 189–204 (I. Malek et al., eds.). London: Academic Press 1969

    Google Scholar 

  • Huber, T. J., Street, J. R., Bull, A. T., Cook, K. A., Cain, R. B.: Aromatic metabolism in the fungi. Growth ofRhodotorula mucilaginosa inp-hydroxybenzoate-limited chemostats and the effect of growth rate on the synthesis of enzymes of the 3-oxoadipate pathway. Arch. Microbiol.102, 139–144 (1975)

    PubMed  Google Scholar 

  • Lelliott, R. A., Billing, E., Hayward, A. C.: A determinative scheme for the fluorescent plant pathogenic Pseudomonads. J. Appl. Bact.29, 470–489 (1966)

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)

    PubMed  Google Scholar 

  • Perley, A. F., Page, D. T.: Differential induction of pectolytic enzymes ofFusarium roseum. Can. J. Microbiol.17, 415–420 (1971)

    PubMed  Google Scholar 

  • Rombouts, F. M.: Occurrence and properties of bacterial pectate lyases. Ph. D. Thesis, Agricultural University, Wageningen, The Netherlands (1972)

    Google Scholar 

  • Rombouts, F. M., Pilnik, W.: Research on pectin depolymerases in the sixties; a literature review. C.R.C. Crit. Rev. Food Technol.3, 1–26 (1972)

    Google Scholar 

  • Rowley, B. I., Bull, A. T.: A chemostat for the cultivation of moulds. Lab. Pract.22, 286–289 (1973)

    PubMed  Google Scholar 

  • Rowley, B. I., Bull, A. T.: Isolation of a yeast-lysingArthrobacter species and the production of the lytic enzyme complex in batch and continuous-flow fermenters. Biotechnol. Bioeng.19, 1260–1278 (1977)

    Google Scholar 

  • Senior, E., Bull, A. T., Slater, J. H.: Enzyme evolution in a microbial community growing on the herbicide Dalapon. Nature, Lond.263, 476–479 (1976)

    Google Scholar 

  • Tejerina, G., Portoles, A., Cabezas de Herrera, E.: Production de enzimas pectoliticas en cultivo continuo. Microbiol. Espan.20, 233–247 (1967)

    Google Scholar 

  • Voragen, A. G. J.: Characterization of pectin lyases on pectins and oligogalacturonates. Ph. D. Thesis, Agricultural University, Wageningen, The Netherlands (1972)

    Google Scholar 

  • Waravdekar, V. S., Saslaw, L. D.: A sensitive colourimetric method for the estimation of 2-deoxy sugars with the use of malonaldehydethiobarbituric acid reaction. J. Biol. Chem.234, 1945–1950 (1959)

    PubMed  Google Scholar 

  • Wieringa, K. T.: A method for isolating and counting pectolytic microbes. Proc. Fourth Int. Cong. Microbiol., Copenhagen, p.482 (1949)

Download references

Author information

Authors and Affiliations

  1. 30 calle 15-31, Zona 12, Guatemala City, C.A.

    M. Leticia Almengor-Hecht

  2. Department of Applied Biology, University of Wales Institute of Science and Technology, CF1 3NU, Cardiff, Wales, UK

    Alan T. Bull

Authors
  1. M. Leticia Almengor-Hecht
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alan T. Bull
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Almengor-Hecht, M.L., Bull, A.T. Continuous-flow enrichment of a strain ofErwinia carotovora having specificity for highly methylated pectin. Arch. Microbiol. 119, 163–166 (1978). https://doi.org/10.1007/BF00964268

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00964268

Key words

Search

Navigation