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Rates of exchange of free amino acids between plasma and brain in mice

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Abstract

The rate of appearance of label in the brain in mice following the intraperitoneal or intravenous injection of tracer doses of amino acids was measured in short time periods (1–8 min). Amino acid flux varied between 2 and 10 nmol/min per g brain for the amino acids used. Defining half-life as the uptake of labeled amino acid amounting to 50% of endogenous levels, a short half-life (between 3 and 30 min) was found for the essential amino acids. The half-life of the nonessential amino acids varied between 2 and 24 h, depending on their level in brain. Flux (exchange) of an amino acid was increased when the level of amino acids belonging to the same transport class was increased by intracerebral injection. Protein-free diet resulted in decrease in some amino acids, increase in others; flux was altered parallel to changes in brain levels in animals on this diet. The stercospecificity of exchange and the substrate specificity of effects of altered brain amino acids indicate that exchange occurs via mediated transport. Mediated exchange was present in immature brain. Heteroexchange (flow of one amino acid causing the counterflow of a related amino acid) may play an important part in cerebral homeostasis.

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References

  1. Lajtha, A., andMela, P. 1961. The brain barrier system. I. The exchange of free amino acids between plasma and brain.J. Neurochem. 7:210–217.

    Google Scholar 

  2. Seta, K., Sershen, H., andLajtha, A. 1972. Cerebral amino acid uptakein vivo in newborn mice.Brain Research 47:415–425.

    Google Scholar 

  3. Lajtha, A. 1977. Transport of amino acids in the nervous system.Physiol. Rev. 57: (in press).

  4. Oldendorf, W. H. 1971. Brain uptake of radiolabeled amino acids, amines, and hexoses after arterial injection.Am. J. Physiol. 221:1629–1639.

    Google Scholar 

  5. Van den Berg, C. J., andGarfinkel, D. 1971. A simulation study of brain compartments. Metabolism of glutamate and related substances in mouse brain.Biochem. J. 123:211–218.

    Google Scholar 

  6. Berl, S., Clarke, D. D., andSchneider, D. (eds.). 1975. Metabolic compartmentation and neurotransmission. Relation to structure and function. NATO Advanced Study Series A, Vol. 6, Plenum Press, New York.

    Google Scholar 

  7. Neidle, A., Kandera, J., andLajtha, A. 1975. Compartmentation and exchangeability of brain amino acids: Evidence from studies of transport into tissue slices.Arch. Biochem. Biophys. 169:397–405.

    Google Scholar 

  8. Seta, K., Sansur, M., andLajtha, A. 1973. The rate of incorporation of amino acids into brain proteins during infusion in the rat.Biochim. Biophys. Acta 294:472–480.

    Google Scholar 

  9. Banay-Schwartz, M., Gergely, A., andLajtha, A. 1974. Independence of amino acid uptake from tissue swelling in incubated slices of brain.Brain Research 65:265–276.

    Google Scholar 

  10. Sadasivudu, B., andLajtha, A. 1970. Metabolism of amino acids in incubated slices of mouse brain.J. Neurochem. 17:1299–1311.

    Google Scholar 

  11. Lajtha, A., Furst, S., Gerstein, A., andWaelsch, H. 1957. Amino acid and protein metabolism of the brain. I. Turnover of free and protein bound lysine in brain and other organs.J. Neurochem. 1:289–300.

    Google Scholar 

  12. Gaitonde, M. K., andRichter, D. 1966. Changes with age in the utilization of glucose carbon in liver and brain.J. Neurochem. 13:1309–1316.

    Google Scholar 

  13. Roberts, S. 1974. Effects of amino acid imbalance on amino acid utilization, protein synthesis and polyribosome function in cerebral cortex. Pages 299–324,in Aromatic amino acids in the brain, CIBA Foundation Symposium 22, American Elsevier, New York.

    Google Scholar 

  14. Felig, P., Wahren, J., andAhlborg, G. 1973. Uptake of individual amino acids by the human brain.Proc. Soc. Exptl. Biol. Med. 142:230–231.

    Google Scholar 

  15. Knauff, H. G., Gottstein, U., andMiller, B. 1964. Untersuchungen über den Austausch von freien Aminosäuren und Harnstoff Blut und Zentralnervensystem.Klin. Wschr. 42:27–39.

    Google Scholar 

  16. Lajtha, A., Latzkovits, L., andToth, J. 1976. Comparison of turnover rates of proteins of the brain, liver, and kidney in mouse in vivo following long-term labeling.Biochim. Biophys. Acta 425:511–520.

    Google Scholar 

  17. Lajtha, A., andToth, J. 1966. Instability of cerebral proteins.Biochem. Biophys. Research Comm. 23:294–298.

    Google Scholar 

  18. Blasberg, R., Levi, G., andLajtha, A. 1970. A comparison of inhibition of steady state, net transport and exchange fluxes of amino acids in brain slices.Biochim. Biophys. Acta 203:464–483.

    Google Scholar 

  19. Battistin, L., Piccoli, F., andLajtha, A. 1972. Heteroexchange of amino acids in incubated slices of brain.Arch. Biochem. Biophys. 151:102–111.

    Google Scholar 

  20. Benjamin, A. M., andQuastel, J. H. 1976. Cerebral uptakes and exchange diffusionin vitro ofl- andd-glutamates.J. Neurochem. 26:431–441.

    Google Scholar 

  21. Levi, G., Cherayil, A., andLajtha, A. 1965. Cerebral amino acid transportin vitro. III. Heterogeneity of exit.J. Neurochem. 12:757–770.

    Google Scholar 

  22. Lajtha, A. 1974. Amino acid transport in the brainin vivo andin vitro. Pages 25–49,in Aromatic acids in the brain, Ciba Foundation Symposium 22, American Elsevier, New York.

    Google Scholar 

  23. Oldendorf, W. H., andSzabo, J. 1976. Amino acid assignment to one of three blood-brain barrier amino acid carriers.Am. J. Physiol. 230:94–98.

    Google Scholar 

  24. Sershen, H., andLajtha, A. 1977. Capillary transport of amino acids in the developing brain.Exptl. Neurol. 54:

  25. Christensen, H. N. 1975. Biological transport (2nd ed.), W. A. Benjamin, Inc., Reading, Massachusetts.

    Google Scholar 

  26. Enwonwu, C. O., andWorthington, B. S. 1974. Regional distribution of homocarnosine and other ninhydrin-positive substances in brains of malnourished monkeys.J. Neurochem. 22:1045–1052.

    Google Scholar 

  27. Lajtha, A. 1968. Transport as control mechanism of cerebral metabolite levels. Pages 201–218,in Lajtha, A., andFord, D. H. (eds.), Brain barrier systems. Elsevier, Amsterdam.

    Google Scholar 

  28. Rech, R. H., andWeichsel, M. E., Jr. 1973. Brain cell number and motor activity in rats subjected to neonatal undernutrition.Life Sci. 13:1077–1087.

    Google Scholar 

  29. Winick, M. 1974. Malnutrition and the developing brain. Pages 252–262,in Plum, F. (ed.). Brain dysfunctions in metabolic disorders, Ass. Res. Publ. Assoc. Nerv. Ment. Dis., Vol. 53, Raven Press, New York.

    Google Scholar 

  30. Garlick, P. J., Millward, D. J., James, W. P. T., andWaterlow, J. C. 1975. The effect of protein deprivation and starvation on the rate of protein synthesis in tissues of the rat.Biochim. Biophys. Acta 414:71–84.

    Google Scholar 

  31. Roach, M. K., Corbin, J., andPennington, W. 1974. Effect of undernutrition on amino acid compartmentation in the developing rat brain.J. Neurochem. 22:521–528.

    Google Scholar 

  32. Abdi, S. A., Morse, E. L., andMirsky, I. A. 1976. Dietary regulation of liver and muscle transport of amino acid.Am. J. Physiol. 230:245–250.

    Google Scholar 

  33. Cheneval, J. P., andJohnstone, R. M. 1976. Changes in amino acid transport in the rat pancreas in response to fasting and feeding.Biochim. Biophys. Acta 433:630–637.

    Google Scholar 

  34. Gan, J. C., andJeffay, H. 1971. The kinetics of transfer of plasma amino acids to tissue and the turnover rates of liver and muscle proteins.Biochim. Biophys. Acta 232:125–135.

    Google Scholar 

  35. Levi, G., andRaiteri, M. 1974. Exchange of neurotransmitter amino acid at endings can simulate high affinity uptake.Nature 250:735–737.

    Google Scholar 

  36. Raiteri, M., Federico, R., Coletti, A., andLevi.G. 1975. Release and exchange studies relating to the synaptosomal uptake of GABA.J. Neurochem. 24:1243–1250.

    Google Scholar 

  37. Okamato, K., andQuastel, J. H. 1972. Uptake and release of glutamate in cerebralcortex slices from the rat.Biochem. J. 128:1117–1124.

    Google Scholar 

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  1. Institute for Neurochemistry and Drug Addiction, Rockland Research Institute, Ward's Island, 10035, New York, N.Y.

    Jeno Toth & Abel Lajtha

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  1. Jeno Toth
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  2. Abel Lajtha
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Toth, J., Lajtha, A. Rates of exchange of free amino acids between plasma and brain in mice. Neurochem Res 2, 149–160 (1977). https://doi.org/10.1007/BF00964093

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