Abstract
Monoamine oxidase in the vervet monkey showed greater variations in activity in six brain regions when tyramine or phenylethylamine was used as the substrate (3.8- to 4.1-fold differences) than when serotonin was the substrate (1.8-fold differences). With phenylethylamine and tyramine as substrates, the highest MAO specific activities were found in the hypothalamus and the lowest in the cerebellum and cortex. With serotonin as the substrate, the highest specific activities were in the mesencephalon and cortex. The inhibition of tyramine deamination by clorgyline and deprenyl yielded biphasic plots indicative of the presence of MAO-A and MAO-B enzyme forms in the vervet brain. On the basis of these inhibitor curves, the vervet brain could be estimated to contain approximately 85% MAO-B and 15% MAO-A, in contrast to rat brain which contains 45% MAO-B and 55% MAO-A. The inhibition of serotonin deamination by deprenyl in vervet brain yielded a biphasic plot, suggesting that some serotonin deamination in the vervet is accomplished by the MAO-B enzyme form. Estimations of the relative amounts of MAO-A and MAO-B based on inhibitor curves or based on substrate ratios yielded proportionate results which were in close agreement across the different brain regions, supporting the validity of these approaches to estimating MAO-A and MAO-B activities.
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References
Johnston, J. P. 1968. Some observations upon a new inhibitor of monoamine oxidase in brain tissue. Biochem. Pharmacol. 17:1285–1297.
Housley, M. D., Tipton, K. F., andYoudim, M. B. H. 1976. Multiple forms of monoamine oxidase: Fact and artifact. Life Sci. 19:467–478.
Hall, D. W. R., Logan, B. W., andParsons, G. H. 1969. Further studies on the inhibition of monoamine oxidase by M & B 9302 (clorgyline)—I. Substrate specificity in various mammalian species. Biochem. Pharmacol. 18:1447–1454
Glover, V., Sandler, M., Owen, F., andRiley, G. J. 1977. Dopamine is a monoamine oxidase B substrate in man. Nature 265:80–81.
Donnelly, C. H., andMurphy, A. T. 1977. Substrate- and inhibitor-related characteristics of human platelet monoamine oxidase. Biochem. Pharmacol. 26:853–858.
White, H. L., andGlassman, A. T. 1977. Multiple binding sites of human brain and liver monoamine oxidase: Substrate specificties, selective inhibitions, and attempts to separate enzyme forms. J. Neurochem. 29:987–997.
Oommen, A. andBalasubramanian, A. S. 1977. Studies on the monoamine oxidase of monkey brain. J. Neurochem. 28:645–654.
McGuire, M. T. 1974. The St. Kitts Vervet, S. Karger, Basel, Switzerland; J. Med. Primatol. 3: 285–297, 1974.
Murphy, D. L., Donnelly, C. H., Baulu, J., andRedmond, D. E., Jr. 1978.MAO type B is the predominant monoamine oxidase form in primate brain (submitted).
Murphy, D. L. 1978. Substrate-selective monoamine oxidases: inhibitor, tissue, species and functional differences. Biochem. Pharmacol., 27:1889–1893.
Maas, J. W., Dekirmenjian, H., Garver, D., Redmond, D. E., Jr., andLandis, D. H. 1973. Excretion of catecholamine metabolites following intraventricular injection of 6-hydroxydopamine in the macaca speciosa. Eur. J. Pharmacol. 23:121–130.
Donnelly, C. H., Richelson, E., andMurphy, D. L. 1976. Properties of monoamine oxidase in mouse neuroblastoma N1E-115 cells. Biochem. Pharmacol. 25:1639–1643.
Tipton, K. F., Houslay, M. D., andMantle, T. J. 1976. Pages 5–16,in, Monoamine Oxidase and Its Inhibition (Ciba Foundation Symp.),J. Knight andG. E. Wolstenholme (eds.). Vol. 39, Elsevier Excerpta Medica, North-Holland, Amsterdam.
Ekstedt, B., andOreland, L. 1976. Effect of lipid-depletion on the different forms of monoamine oxidase in rat liver mitochondria. Biochem. Pharmacol. 25:119–124.
Edwards, D. J., andMalsbury, C. W. 1977. Distribution of types A and B monoamine oxidases in discrete brain regions, pineal and pituitary glands of the golden hamster. Life Sci. 21:1009–1014.
Harada, M., Kondo, Y., Kuzuya, H., andNagatsu, T. 1975. Monoamine oxidase activities towards biogenic monoamines in several regions of rat brain. J. Neurochem. 24:193–195.
Saavedra, J. M., Brownstein, M. J., andPalkovits, M. 1976. Distribution of catechol-O-methyltransferase, histamine n-methyltransferase and monoamine oxidase in specific areas in the rat brain. Brain Res. 118:152–156.
Vogel, W. H., Orfei, V., andCentury, B. 1969. Activities of enzymes involved in the formation and destruction of biogenic amines in various areas of human brain. J. Pharmacol. Exp. Ther. 165:196–203.
Collins, G. G. S., Sandler, M., Williams, E. D., andYoudim, M. B. H. 1970. Multiple forms of human brain mitochondrial monamine oxidase. Nature 225:817–820.
Gottfries, C. G., Oreland, L., Winberg, A., andWinblad, B. 1975. Lowered monoamine oxidase activity in brains from alcoholic suicides. J. Neurochem. 25:667–673.
Hiramo, M., Uchimura, H., andSaito, M. 1975. Regional distribution of monoamine oxidase activity for 5-hydroxytryptamine and tyramine in hypothalamus of the rat. Brain Res. 93:558–563.
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Murphy, D.L., Redmond, D.E., Garrick, N. et al. Brain region differences and some characteristics of monoamine oxidase type a and b activities in the vervet monkey. Neurochem Res 4, 53–62 (1979). https://doi.org/10.1007/BF00963831
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DOI: https://doi.org/10.1007/BF00963831