Skip to main content
SpringerLink
Log in

Deposition of extracellular matrix on silicone intraocular lens implants in rabbits

  • Laboratory Investigation
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

• Purpose: To examine the deposition of extracellullar matrix on silicone intraocular lenses (IOLs) implanted experimentally into rabbit eyes by electron microscopy and to determine the immunolocalization of extracellular matrix components, including collagen types and cellular fibronectin, on these IOLs. • Methods: We performed phacoemulsification and aspiration of the crystalline lens and implanted a foldable silicone IOL in the capsular bag of one eye of each of 26 adult albino rabbits under general anesthesia. After 8 weeks the animals were killed and the eyes were enucleated. The silicone IOLs were processed for electron microscopy and for immunohistochemical detection of collagen types I, III, and IV and cellular fibronectin. • Results: Electron microscopy revealed deposition of a presumed cell matrix complex on the optic portion of all silicone IOLs, as well as the adhesion of presumed macrophages and foreign-body giant cells. Cellular deposits showed immunoreactivity for cellular fibronectin. Fibrous or membranous deposits exhibited immunoreactivity for cellular fibronectin and collagen types I and III. A few type IV collagen-immunoreactive deposits were also seen. • Conclusion: Deposits of extracellular matrix components were observed on silicone IOLs. These deposits may form the scaffolding for the adhesion and proliferation of cells. These matrix components appeared to be the products of cells adhering to the surfaces of IOLs, including lens epithelial cells, macrophages and foreign-body giant cells, indicating that the process of granulation was incomplete.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adams DO (1976) The granulomatous inflammatory response. A review. Am J Pathol 84:164–191

    Google Scholar 

  2. Alitalo K, Hovi T, Veheri A (1980) Fibronectin is produced by human macrophages. J Exp Med 151: 602–613

    Google Scholar 

  3. Blotnick CA, Powers TP, Newlans T, McMillan T, Bluestein E, Apple DJ (1995) Pathology of silicone intraocular lenses in human eyes obtained postmortem. J Cataract Refract Surg 21:447–542

    Google Scholar 

  4. Boudouin C, Fredj-Reygrobellet D, Brignole F, Negre F, Lapalus P, Gastaud P (1993) Growth factors in vitreous and subretinal fluid cells from patients with proliferative vitreoretinopathy. Ophthalmic Res 25: 52–59

    Google Scholar 

  5. Connor TB jr, Roberts AB, Sporn MB, Danielpour D, Dart LL, Mivhels RG, de Bustros S, Enger C, Kato H, Lausing M, Hayashi H, Glaser BM (1989) Correlation of fibrosis and transforming growth factor-β type 2 levels in the eye. J Clin Invest 83:1661–1666

    Google Scholar 

  6. D'Ardenne AJ, McGee JO (1984) Fibronectin in disease. J Pathol 142:235–254

    Google Scholar 

  7. Gonzalez-Avila G, Lozano D, Manjarrez M-E, Ruiz VM, Teran L, Vadillo-Ortega F, Selman M (1995) Influence on collagen metabolism of vitreous from eyes with proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 102:1400–1405

    Google Scholar 

  8. Holund B, Clemmensen I, Junker P, Lyon H (1982) Fibronectin in experimental granulation tissue. APMIS [A] 90:159–165

    Google Scholar 

  9. Ishibashi T, Sugai S, Kubota T, Ohnishi Y, Inomata H (1989) Cytopathology of early cellular response on implanted lenses in monkeys. Graefe's Arch Clin Exp Ophthalmol 227:470–475

    Google Scholar 

  10. Ishibashi T, Sugai S, Kubota T, Ohnishi Y, Inomata H (1990) Cellular adhesiveness on implanted lenses in monkeys. Graefe's Arch Clin Exp Ophthalmol 228: 356–362

    Google Scholar 

  11. Ishibashi T, Sugai S, Ohnishi Y, Inomata H, Yashitomi F, Sasaki K (1990) Cellular reaction on the surface of intraocular lenses removed from human eyes. J Cataract Refract Surg 16:751–754

    Google Scholar 

  12. Ishibashi T, Araki H, Sugai S, Tawara A, Ohnishi Y, Inomata H (1993) Anterior capsule opacification in monkey eyes with posterior chamber intraocular lenses. Arch Ophthalmol 111:1685–1690

    Google Scholar 

  13. Ishibasbi T, Hatae T, Inomata H (1994) Collagen types in human posterior capsule opacification. J Cataract Refract Surg 20:643–646

    Google Scholar 

  14. Ishibashi T, Araki H, Sugai S, Tawara A, Inomata H (1995) Detection of proteoglycans in human posterior capsule opacification. Ophthalmic Res 27:208–213

    Google Scholar 

  15. Kanagawa R, Ohmi S, Tamura M, Uenoyama K (1989) Immunohistochemical study on cellular response on the intraocular lens surface. Jpn J Ophthalmol 33:337–342

    Google Scholar 

  16. Kanagawa R, Saika S, Ohmi S, Tamura M, Nakao T (1990) Presence and distribution of fibronectin on the surface of implanted intraocular lenses in rabbits. Graefe's Arch Clin Exp Ophthalmol 228:398–400

    Google Scholar 

  17. Kappelhof JP, Pameyer JH, De Jong PTVM, Jongkind JF, Vrensen GFJM (1986) The proteinaceous coating and cytology of implant lenses in rabbits. Am J Ophthalmol 102:750–758

    Google Scholar 

  18. Kochounian HH, Kovacs SA, Sy J, Grubbs DE, Maxwell WA (1996) Identification of intraocular lens adsorbed proteins in mammalian in vitro and in vivo systems. Arch Ophthalmol 112:395–401

    Google Scholar 

  19. Kurkinen M, Vaheri A, Roberts PJ, Stenman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51

    Google Scholar 

  20. Laurent M, Kern P, Courtois Y, Regamult F (1981) Synthesis of types I, III, and IV collagen by bovine lens epithelial cells in long-term culture. Exp Cell Res 134:23–31

    Google Scholar 

  21. McDonnell PJ, Stark WJ, Green WR (1983) Posterior capsule opacification: a specular microscopic study. Ophthalmology 91:853–856

    Google Scholar 

  22. Miyake K, Asakura M, Kobayashi H (1984) Effect of intraocular lens fixation on the blood aqueous barrier. Am J Ophthalmol 98:588–590

    Google Scholar 

  23. Ramaekers ECS, Osborn M, Weber K, Bloemandal H, Franke WW (1980) Identification of the cytoskeletal proteins in lens-forming cells, a special epitheliloid cell type. Exp Cell Res 127:309–327

    Google Scholar 

  24. Repesh LA, Fitzgerald TJ, Furcht LT (1982) Fibronectin involvement in granulation tissue and wound healing in rabbits. J Histochem Cytochem 30:351–358

    Google Scholar 

  25. Saika S, Tamura M, Nakao T, Kanagawa R, Uenoyama K, Hiraoka J (1991) Experimental study of phagocytic activity of cells observed on implanted intraocular lenses. Eur J Implant Refract Surg 3:87–93

    Google Scholar 

  26. Saika S, Tamura M, Uenoyama K, Yamanaka A, Ohkubo K, Iwane H (1992) Collagenous deposits on explanted intraocular lenses. J Cataract Refract Surg 18:195–199

    Google Scholar 

  27. Saika S, Tonoe O, Kanagawa R, Uenoyama K, Yamanaka A, Fukuda K, Iwane H (1992) Immunohistochemical study of deposits on intraocular lenses explanted from human eyes. Jpn J Ophthalmol 35:96–101

    Google Scholar 

  28. Saika S, Uenoyama K, Hiroi K, Ooshima A (1992)l-Ascorbic acid 2phosphate enhances the production of type I and type III collagen peptides in cultured rabbit keratocytes. Ophthalmic Res 24:68–72

    Google Scholar 

  29. Saika S, Uenoyama S, Kanagawa R, Tamura M, Uenoyama K (1992) Phagocytosis and fibronectin of cells observed on intraocular lenses. Jpn J Ophthalmol 36:184–191

    Google Scholar 

  30. Saika S, Kobata S, Yamanaka O, Yamanaka A, Okubo K, Oka T, Hosomi M, Kano Y, Uenoyama S, Tamura M, Kanagawa R, Uenoyama K (1993) Cellular fibronectin on intraocular lenses explanted from patients. Graefe's Arch Clin Exp Ophthalmol 231:718–721

    Google Scholar 

  31. Saika S, Yamanaka A, Ohmi S, Tanaka S, Ohnishi Y, Ooshima A (1995) Extracellular matrix on intraocular lenses. Exp Eye Res 61:713–721

    Google Scholar 

  32. Saika S, Ohmi S, Kanagawa R, Tanaka S, Ohnishi Y, Ooshima A, Yamanaka A (1996) Outgrowth of lens epithelial cells and matrix formation on implanted intraocular lenses in rabbits. J Cataract Refract Surg 22:835–840

    Google Scholar 

  33. Sakamoto T, Ishibashi T, Sugai S, Ohnishi Y, Fukushima M, Sueishi K (1990) Immunohistochemical study of the surface membrane on an intra-ocular lens. (in Japanese) Nippon Ganka Gakkai Zashi 94:1074–1078

    Google Scholar 

  34. Sanders DR, Kraff MC, Lieberman HL, Payman GA, Tarabishy S (1982) Breakdown and reestablishment of blood-aqueous barrier with implant surgery. Arch Ophthalmol 100:588–590

    Google Scholar 

  35. Uenoyama K, Matoba M, Kanagawa R, Enomoto Y, Tamura M, Ohmi S (1988) Experimental intraocular lens implantation in the rabbit eye and in the mouse peritoneal space. I, II, and III. J Cataract Refract Surg 14:187–201

    Google Scholar 

  36. Van der Rhee HJ, Van der Burgh-deWinter CPM, Daems WTh (1979) The differentiation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells in subcutaneous granulomas. I and II. Cell Tissue Res 197:355–396

    Google Scholar 

  37. Van der Rhee HJ, Van der Burgh-de Winter CPM, Tijissen JGP, Daems WTh (1979) Comparative study on peroxidatic activity in inflammatory cells on cutaneous and peritoneal implants. Cell Tissue Res 197:397–412

    Google Scholar 

  38. Vartio T, Laitnen L, Narvanen O, Cutolo M, Thornell L-E, Zardi L, Virtanen I (1987) Differential expression of the ED sequence-containing form of cellular fibronectin in embryonic and adult human tissue. J Cell Sci 88:419–439

    Google Scholar 

  39. Wolter JR (1985) Cytopathology of intraocular lens implantation. Ophthalmology 92:135–142

    Google Scholar 

  40. Wolter JR (1993) Continuous sheet of lens epithelium on an intraocular lens: pathological confirmation of specular microscopy. J Cataract Refract Surg 19:789–792

    Google Scholar 

  41. Yang CM, Cousins SW (1992) Quantitative assessment of growth stimulating activity of the vitreous during PVR. Invest Ophthalmol Vis Sci 33:2436–2442

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Wakayama Medical College, 7-Bancho 27, 640, Wakayama, Japan

    Shizuya Saika, Shunsaku Ohmi, Sai-ichi Tanaka, Yuka Okada & Yoshitaka Ohnishi

  2. Department of Pathology, Wakayama Medical College, Wakayama, Japan

    Akira Ooshima

  3. Department of Anatomy, Kansai Shinkyu College, Sennan, Osaka, Japan

    Michiro Kimura

  4. Department of Ophthalmology, Kobe Kaisei Hospital, Kobe, Japan

    Akio Yamanaka

Authors
  1. Shizuya Saika
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shunsaku Ohmi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akira Ooshima
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michiro Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sai-ichi Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuka Okada
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yoshitaka Ohnishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Akio Yamanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saika, S., Ohmi, S., Ooshima, A. et al. Deposition of extracellular matrix on silicone intraocular lens implants in rabbits. Graefe's Arch Clin Exp Ophthalmol 235, 517–522 (1997). https://doi.org/10.1007/BF00947010

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00947010

Keywords

Search

Navigation