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Effect of PT-treatment on ANP-mediated inhibition of adenylate cyclase and amylase release in rat parotid gland

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Abstract

Effects of pertussis toxin (PT) treatment on atrial natriuretic peptide (ANP)-mediated inhibition of adenylate cyclase and amylase release were investigated in rat parotid gland. Adenylate cyclase activity stimulated by GTPγS in PT-treated membranes was much larger than that in normal membranes. ANP dose-dependently inhibited adenylate cyclase stimulated by GTPγS in control rat parotid membranes, however in membranes prepared from PT-injected (in vivo) rat parotid gland, ANP did not inhibit adenylate cyclase. ANP(10−7M) inhibited cAMP accumulation stimulated by forskolin (10−6M) in control rat parotid acinar cells by about 34%, however, in PT-treated cells, the inhibitory effect of ANP was attenuated completely. In control cells, amylase release stimulated by isoproterenol (10−6M) and forskolin (10−6M) were also depressed by ANP (10−7M) by 27 and 30%, respectively. The inhibitory response of ANP on amylase release was completely attenuated by PT-treatment. Gi was detected as a ADP-ribosylated 41-KDa protein by incubation of parotid membranes with PT and [α-32P]NAD. In rat parotid gland, these results suggested that ANP mediates adenylate cyclase/cAMP system and consequently reduces amylase release through ANP-C receptor coupled to Gi. (Mol Cell Biochem)139: 53–58, 1994)

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References

  1. Chinkers M, Garbers DL, Chang MS, Lowe DG, Chin H, Goeddel DV, Schulz S: A membrane form of guanylate cyclase is an atrial natriuretic peptide receptor. Nature 338: 78–83, 1989

    PubMed  Google Scholar 

  2. Schulz S, Singh S, Bellet RA, Singh G, Tubb DJ, Chin H, Gargers DL: The primary structure of a plasma membrane guanylate cyclase demonstrates diversity within this new receptor family. Cell 58: 1155–1162, 1989

    PubMed  Google Scholar 

  3. Fuller F, Porter JG, Arfsten AE, Miller J, Schilling JW, Scarborough RM, Lewicki JA, Schenk DB: Atrial natriuretic peptide clearance receptor. J Biol Chem 263: 9395–9401, 1988

    PubMed  Google Scholar 

  4. Wilcox JN, Augustine A, Goeddel DV, Lowe DG: Differential regional expression of three natriuretic peptide receptor genes within primate tissue. Mol Cell Biol 11:3454–3462, 1991

    PubMed  Google Scholar 

  5. Inagami T: Atrial natriuretic factor. J Biol Chem 265: 3043–3046, 1989.

    Google Scholar 

  6. Anand-Srivastava MB, Cantin M, Genest J: Inhibition of pituitary adenylate cyclase by atrial natriuretic facton Life Sciences 36: 1873–1879, 1985

    PubMed  Google Scholar 

  7. Anand-Srivastava MB, Genest J, Cantin M: Inhibitory effect of atrial natriuretic factor on adenylate cyclase activity in adrenal cortical membranes, FEBS Letters 181: 199–202, 1985

    PubMed  Google Scholar 

  8. Anand-Srivastava MB, Franks DJ, Cantin M, Genest J: Atrial natriuretic factor inhibits adenylate cyclase activity. Biochem Biophys Res Commun 121: 855–862, 1984

    PubMed  Google Scholar 

  9. Anand-Srivastava MB, Cantin M: Atrial natriuretic factor receptors are negatively coupled to adenylate cyclase in cultured atrial and ventricular cardiocytes. Biochem Biophys Res Commun 138: 427–436, 1986

    PubMed  Google Scholar 

  10. Anand-Srivastava MB, Vinay P, Genest JM: Effect of atria natriuretic factor on adenylate cyclase in various nephron segments. Am J Physiol 251: F417-F423, 1986

    PubMed  Google Scholar 

  11. Anand-Srivastava MB, Sairam MR, Cantin M: Ring-deleted analogs of atrial natriuretic factor inhibit adenylate cyclase/cAMP system. J Biol Chem 265: 8566–8572, 1990

    PubMed  Google Scholar 

  12. Anand-Srivastava MB, Srivastava AK, Cantin M: Pertussis toxin attenuates atrial natriuretic factor-mediated inhibition of adenylate cyclase. J Biol Chem 262: 4931–4934, 1987

    PubMed  Google Scholar 

  13. Okajima F, Kondo Y: Inhibition of atrial natriuretic peptide-induced cGMP accumulation by purinergic agonists in FRTL-5 thyroid cells. J Biol Chem 265: 21741–21748, 1990

    PubMed  Google Scholar 

  14. Yabe T, Imai A, Nashida T, Shimomura H: A study of atrial natriuretic peptide receptor of the rat salivary gland. Odontology 80:1441–1449, 1993

    Google Scholar 

  15. Nashida T, Imai A, Shimomura H: Influence of atrial natriuretic peptide on cyclic nucleotides and amylase release in rat parotid salivary glandin vitro. Archs Oral Biol: 39: 629–632, 1994

    Google Scholar 

  16. Katada T, Ui M: Islet-activating protein. J Biol Chem 254: 469–479, 1979

    PubMed  Google Scholar 

  17. Salomon Y, Londos C, Rodbell M: A highly sensitive adenylate cyclase assay. Anal Biochem 58: 541–548, 1974

    PubMed  Google Scholar 

  18. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227: 680–685, 1970

    PubMed  Google Scholar 

  19. Bernfeld P: Amylase, α and β. In: SP Colowick and NO Kaplan (eds). Methods Enzymol Vol. 1, Academic Press, New York, 1955, pp 149–158

    Google Scholar 

  20. Goodfriend TL, Elliott ME, Atlas SA: Actions of synthetic atrial natriuretic factor on bovine adrenal glomerulosa. Life Sci 35: 1675–1682, 1984

    PubMed  Google Scholar 

  21. Obana K, Naruse M, Naruse K, Sakurai H, Demura H, Inagami T, Shizume K: Synthetic rat atrial natriuretic factor inhibitsin vitro andin vivo renin secretion in rats. Endocrinology 117: 1282–1284, 1985

    PubMed  Google Scholar 

  22. Samson WK: Atrial natriuretic factor inhibits dehydration and hemorhage-induced vasopressin release. Neuroendocrinology 40: 277–279, 1985

    PubMed  Google Scholar 

  23. Meloche S, Ong H, Cantin M, De Lean CR: Molecular characterization of the solubilized atrial natriuretic factor receptor from bovine adrenal zona glomerulosa. Mol Pharmacol 30: 537–543, 1986

    PubMed  Google Scholar 

  24. Resink TJ, Scott-Burden T, Baur U, Jones CR, Buhler FR: Atrial natriureic peptide induces breakdown of phosphatidyl inositol phosphates in cultured vascular smooth-muscle cells. Eur J Biochem 172: 499–505, 1988

    PubMed  Google Scholar 

  25. Meloche S, Ong H, Cantin M, De Lean A: Affinity cross-linking of atrial natriuretic factor to its receptor in bovine adrenal zona glomerulosa. J Biol Chem 261: 1525–1528, 1986

    PubMed  Google Scholar 

  26. Resink TJ, Panchenko MP, Tkachuk VA, Buhler RF: Involvement of Ni protein in the functional coupling of the atrial natriuretic factor (ANF) receptor to adenylate cyclase in rat lung plasma membranes. Eur J Biochem 174: 531–535, 1988

    PubMed  Google Scholar 

  27. Anand-Srivastav MB: Characterization of ANF-R2 receptor-mediated inhibition of adenylate cyclase. Mol Cell Biochem 113: 83–92, 1992

    PubMed  Google Scholar 

  28. Watson EL, DiJulio D, Kauffman D, Iversen J, Robinovitch MR, Izutsu KT. Evidence for G proteins in rat parotid plasma membranes and secretory granule membranes. Biochem J 285: 441–449, 1992

    PubMed  Google Scholar 

  29. Sawaki K, Hiramatsu Y, Baum BJ, Ambudkar I.S.: Involvement of Gαq/11 in m3-muscarinic receptor stimulation of phosphatidy linositol 4,5 bisphosphate-specific phospholipase C in rat parotid gland membranes. Arch Biochem Biophys 305: 546–550, 1993

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Oral Biochemistry, Nippon Dental University, School of Dentistry at Niigata, 1-8 Hamaura-cho, 951, Niigata, Japan

    Hiromi Shimomura, Tomoko Nashida & Akane Imai

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  1. Hiromi Shimomura
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  2. Tomoko Nashida
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  3. Akane Imai
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Shimomura, H., Nashida, T. & Imai, A. Effect of PT-treatment on ANP-mediated inhibition of adenylate cyclase and amylase release in rat parotid gland. Mol Cell Biochem 139, 53–58 (1994). https://doi.org/10.1007/BF00944203

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