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Lack of effects of recombinant human interleukin-4 onin vitro colony formation of freshly explanted human tumor cells

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Summary

Interleukin-4 is a highly pleiotropic T-cell derived lymphokine that has been reported to stimulate a host cell-mediated antitumor response. Recombinant human interleukin-4 (rhuIL-4) is currently undergoing clinical phase I trials. We have studied the growth modulating effects of rhuIL-4 on a variety of freshly explanted human tumor specimens using anin vitro soft agar cloning system. Final concentrations of 0.1 to 10 ng/ml were used in continuous incubation experiments. Of 147 specimens, 73 (50%) were evaluable for the determination of tumor growth modulating activity. The most common tumor types recruited included breast, nonsmall cell lung, ovarian cancer and melanoma. Stimulation of tumor colony forming units (colony formation ≥1.5× controls) was observed in 0/73 tumors. Similarly, only 1/73 (1.3%) specimens (a non-small cell lung cancer) had a significant decrease in tumor colony forming units (colony formation ≤ 0.5× controls) at 1 ng/ml. We conclude that rhuIL-4 is not a direct modulator of tumor colony formationin vitro. However, antitumor effects could perhaps be achievedin vivo via the immune-modulating effects of Interleukin-4.

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Authors and Affiliations

  1. Abteilung Hämatologie und Onkologie, Technische Universität München, Klinikum rechts der Isar, Germany

    Axel -R. Hanauske

  2. Section of Drug Development, Division of Oncology, Department of Medicine, University of Texas Health Science Center at San Antonio and Cancer Therapy and Research Center, 78229, San Antonio, TX, USA

    Donna Degen, Martha H. Marshall & Daniel D. Von Hoff

  3. Schering-Plough Corporation, 60 Orange Street, NJ 07003, Bloomfield, USA

    Paul P. Trotta

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  1. Axel -R. Hanauske
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  2. Donna Degen
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  3. Martha H. Marshall
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  4. Paul P. Trotta
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  5. Daniel D. Von Hoff
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Hanauske, A.R., Degen, D., Marshall, M.H. et al. Lack of effects of recombinant human interleukin-4 onin vitro colony formation of freshly explanted human tumor cells. Invest New Drugs 10, 269–273 (1992). https://doi.org/10.1007/BF00944180

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