Summary
Two auxotrophic mutants ofRhizobium trifolii which are deficient in nodulating ability have been isolated. Both mutants (strain RS 164 His− and strain RS213 Leu−) appear to synthesize abnormal extracellular polysaccharides as compared with the wild type strain RS 55. Simultaneous recovery of nodulating ability and wild type polysaccharide composition has been found in a Leu+ revertant of strain RS 213.
Similar content being viewed by others
Abbreviations
- EPS:
-
Extracellular Polysaccharide
- NIG:
-
N-Methyl-N-Nitro-N-Nitrosoguanidine
References
Amarger N, Obaton M, Blachere H (1967) Polysaccharides extracellulaires deRhizobium meliloti. Can J Microbiol 13: 99–105
Beringer JE (1974) R-factor transfer inRhizobium leguminosarum. J Gen Microbiol 84:188–198
Beringer JE (1981) Genetics of symbiotic nitrogen fixing microorganisms. Proceedings of the Fourth International Symposium of Nitrogen Fixation. Canberra, pp 131–136
Bhagwat A, Thomas J (1980) Dual binding sites for peanut lectin on Rhizobia. J. Gen Microbiol 117:119–125
Cerdá-Olmedo E, Hanawalt PC (1967) Macromolecular action of nitrosoguanidine inEscherichia coli. Biochim Biophys Acta 142: 450–464
Davison PK, Young R (1969) Gas chromatography of carbohydrates. The quantitative determination of the free sugars of plants as their trimethylsilyl ethers. J Chrom 41: 12–21
Dazzo FB, Yanke WE, Brill WJ (1978) Trifollin: aRhizobium recognition protein from white clover. Biochem Biophys Acta 539: 276–286
Dazzo FB, Brill WJ (1979) Bacterial polysaccharide which bindsRhizobium trifolii to clover root hairs. J Bact 137: 1362–1373
Denarié J, Truchet G, Bergeron B (1976) Effects of some mutations on symbiotic properties ofRhizobium. In: Nutman PS (ed) Symbiotic nitrogen fixation in plants. Cambridge University Press, Cambridge, pp 47–61
Denarié J, Truchet G (1979) La symbioseRhizobium-legumineuses; role respectifs des partenaires. Physil Veg 17: 643–667
Denarié J, Truchet G (1979) La symbioseRhizobium-legumineuses; role respectifs des partenaires. Physil Veg 17: 643–667
Fahraeus G, Ljunggren H (1959) Effect ofRhizobium polysaccharide on the formation of polygalacturonase in lucerne and clover. Nature 184: 1578–1579
Hewitt EJ (1952) Sand and water culture methods used in the study of plant nutrition. Tech Comm 22. Commonwealth Agric Bureau
Holliday R (1956) Amino acid, vitamins and purine/pyrimidine pools used in the determination of auxotrophic requirements. Nature 178: 1987
Hombrecher G, Brewin NJ (1979) Studies on the binding of pea seed lectins to species and strains ofRhizobium. International symposium on nitrogen fixation. Brighton
Hooykaas PJJ, Klapwijk PH, Nuti MP, Schilperoort RA, Rorsch A (1977) Transfer of theAgrobacterium tumefaciens TI plasmid to avirulent agrobacteria and toRhizobium ex planta. J Gen Microbiol 98: 477
Kamberger W (1979) An Ouchterlony double diffusion study on the interaction between legume lectins and Rhizobial cell surface antigens. Arch Microbiol 121: 83–90
Ljunggren H (1969) Mechanism and pattern ofRhizobium invasion into leguminous root hairs. Physiol Plant Suppl V
Mokrasch LC (1954) Analysis of hexose phosphate and sugar mixtures with the anthrone reagent. J Biol Chem 208: 55–59
Napoli C, Albersheim P (1980)Rhizobium leguminosarum mutants incapable of normal extracellular polysaccharide production. J Bact 141: 1454–1456
Nordhoff H, Kamberger W (1979) An electron microscopy study on the interaction between legume lectins and rhizobial cell surface antigens. International Symposium on Nitrogen Fixation Brighton
Olivares J, Montoya E, Palomares AJ (1977) Some effects derived from the presence of extrachromosomal DNA inRhizobium meliloti In: Newton WE, Postgate JR, Rodriguez-Barrueco C (eds) Recent developments in nitrogen fixation. Academic, pp 375–385
Palomares, AJ, Montoya E, Olivares J (1978) Quality and rate of extracellular polysaccarides produced byRhizobium meliloti and their inducing effect on polygalacturonase production in legume roots as derived from the presence of extrachromosomal DNA. Microbios 22: 7–13
Quispel A (1974) The endophyte of the root nodules in non-leguminous plants. In: Quispel A (ed) The biology of nitrogen fixation. North-Holland Publ Co, Amsterdam, pp 499–520
Rolfe BG, Gresshoff PM, Shine J, Vincent JM (1980) Interaction between a non-nodulating and an ineffective mutant ofRhizobium trifolii resulting in effective (Nitrogen-Fixing) nodulation. App Env Microbiol 39: 449–452
Sanders RE, Carlson RW, Albersheim P (1978) ARhizobium mutant incapable of nodulation and normal polysaccharide secretion. Nature 271: 240–242
Sanders RE, Releigh E (1981) Lack of correlation between extracellular polysaccharide and nodulation ability inRhizobium. Nature 292: 148–149
Tsien HC, Schmidt EL (1977) Polarity in the exponential-phaseRhizobium japonicum cell. Can J Microbiol 23: 1274–1284
Zelazna-Kowalska I, Lorkiewicz Z (1971) Transformation inRhizobium trifolii. IV. Correlation between streptomycin resistance and infectiveness inRhizobium trifolii. Acta Microbiol Polonica 3: 11–20
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Caviedes, M.A., Megias, M., Gil, A.M. et al. Correlation between extracellular polysaccharide composition and nodulating ability inRhizobium trifolii . Arch. Microbiol. 133, 20–22 (1982). https://doi.org/10.1007/BF00943763
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00943763