Abstract
We have quantified and characterized α1-, α2-and β-adrenoceptor subtypes in porcine bladder detrusor and bladder neck, human bladder detrusor, and porcine and human prostate. α1-, α2- and β-adrenoceptor were identified in radioligand binding studies using [3H]prazosin, [3H]RX 821002 and [125I]iodocyanopindolol, respectively, as the radioligands. In porcine male and female detrusor and bladder neck and male prostate, adrenoceptors were detected in the order of abundance β > α2 ≫ α1 (not detectable), with no major differences between the sexes or between detrusor and bladder neck. In human detrusor and prostate the order of abundance was β > α2 ≫ α1 (not detectable) and β ≫ α1 > α2. respectively. The α2-adrenoceptors in all tissues were homogeneously of the α2A-subtype as evidenced by competition binding studies with yohimbine, prazosin, ARC 239 and oxymetazoline. The β-adrenoceptors represented a mixed population with a dominance of the β2-subtype in all tissues as demonstrated by competition binding with ICI 118,551 and CGP 20,712A. We conclude that pigs may be a suitable model for studies of detrusor function with respect to adrenoceptor expression. They may be less suitable for studies of bladder neck or prostate function.
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Goepel, M., Wittmann, A., Rübben, H. et al. Comparison of adrenoceptor subtype expression in porcine and human bladder and prostate. Urol. Res. 25, 199–206 (1997). https://doi.org/10.1007/BF00941983
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DOI: https://doi.org/10.1007/BF00941983