Abstract
The D genome cluster includes six allopolyploidAegilops species having as pivotal genome that ofAegilops squarrosa. Alpha-gliadins, endosperm proteins coded by multigenic families, have been analyzed in the D genome species cluster and in their putative progenitors. They can be present or weakly expressed when analyzed in acid polyacrylamide gel electrophoresis. Molecular analysis has shown the possibility to distinguish subsp.strangulata from subsp.eusquarrosa and to confirm the presence ofAe. caudata and ofAe. umbellulata in the polyploidsAe. cylindrica andAe. juvenalis, respectively. Finally, introgression fromAe. longissima orAe. searsii in tetraploid and hexaploidAe. crassa, Ae. juvenalis, andAe. vavilovii is supposed.
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References
Chennaveeraiah, M. S., 1960: Karyomorphologic and cytotaxonomic studies inAegilops. — Acta Horti Gotob.23: 85–178.
Croston, R. P., Williams, J. T., 1981: A world survey of wheat genetic resources. — AGP: Int. Board Plant Genet. Resources 80/59.
D'Ovidio, R., Tanzarella, O. A., Porceddu, E., 1991: Isolation of an alpha-type gliadin gene fromTriticum durum Desf. and genetic polymorphism at theGli-2 locus. — Genetics & Breeding (in press).
Dvorak, J., McGuire, P. E., Cassidy, B., 1988: Apparent sources of the A genomes of wheats inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. — Genome30: 680–689.
Eig, A., 1929: Monographisch-kritische Übersicht der GattungAegilops. — Rep. Spec. Nov. Reg. Veg., Beih.55: 1–228.
Feldman, M., Kislev, M., 1978:Aegilops searsii, a new species from Israel and Jordan. — Wheat Inf. Service 45/46.
Hammer, K., 1980a: Zur Taxonomie und Nomenklatur der GattungAegilops L. — Feddes Repert.91: 225–258.
—, 1980b: Vorarbeiten zur monographischen Darstellung von Wildpflanzensortimenten:Aegilops L. — Kulturpflanze28: 33–180.
Jaaska, V., 1981: Aspartate aminotransferase and alcohol dehydrogenase isoenzymes: intraspecific differentiation inAegilops tauschii and the origin of the D genome polyploids in the wheat group. — Pl. Syst. Evol.137: 259–273.
Johnson, B. L., 1967: Confirmation of the genome donors ofAegilops cylindrica. — Science216: 859–862.
Kasarda, D. D., Adalsteins, A. E., Laird, N. F., 1987: Gamma-gliadins with alphatype structure coded on chromosome 6 B of the wheat (Triticum aestivum L.) cultivar “Chinese Spring”. — InLasztity, R., Bekes, F., (Eds.): Proc. 3rd Int. Workshop on Gluten Proteins, pp. 20–29. — Budapest, Hungary.
Khakimova, A. G., 1988: Gliadins in genepool registration ofAegilops squarrosa L. (=Ae. tauschii Coss.). — InKonarev, V. G., Gavriljuk, I. P., (Eds.): Biochemical identification of varieties, pp. 160–164. — Leningrad, USSR.
—, 1973: The component and antigen composition of gliadin in different representatives ofAegilops squarrosa L. — Bull. Appl. Bot., Genet. Breed. (Leningrad)52: 193–205.
Khan, K., Hamada, A. S., Patek, J., 1985: Polyacrylamide gel electrophoresis for wheat variety identification: effect of variables on gel properties. — Cereal Chem.62: 310–313.
Kihara, H., 1954: Considerations on the evolution and distribution ofAegilops species based on the analyzer-method. — Cytologia19: 336–357.
—, 1959: Genomes of 6x species ofAegilops. — Wheat Inform. Service8: 3–5.
Kimber, G., 1983: Genome analysis in the genusTriticum. — In Proc. 6th Int. Wheat Genet. Symp., pp. 23–28. — Kyoto, Japan.
- 1987: Evolutionary patterns in the wheat group. — InLasztity, R., Bekes, F., (Eds.): Proc. 3rd Int. Workshop on Gluten Proteins, pp. 47–51. — Budapest, Hungary.
—, 1987: Wild wheat: an introduction. — Spec. Rep. 353. College of Agriculture. University of Missouri, Columbia.
—, 1983: The D genome of theTriticeae. — Canad. J. Genet. Cytol.25: 581–589.
Lagudah, E. S., Halloran, G. M., 1988a: Phylogenetic relationships ofTriticum tauschii, the D genome donor to hexaploid wheat. 1. Variation in HMW subunits of glutenin and gliadins. — Theor. Appl. Genet.75: 592–598.
—, 1988b: Phylogenetic relationships ofTriticum tauschii, the D genome donor to hexaploid wheat. 2. Inheritance and chromosomal mapping of the HMW subunits of glutenin and gliadin gene loci ofT. tauschii. — Theor. Appl. Genet.75: 592–598.
—, 1987: The influence of high-molecular-weight subunits of glutenin fromTriticum tauschii on flour quality of synthetic hexaploid wheat. — J. Cereal Sci.5: 129–138.
Morris, R., Sears, E. R., 1967: The cytogenetics of wheat and its relatives. — InQuisenberry, K. S., Reitz, L. P., (Eds.): Wheat and wheat improvement, pp. 19–87. — Amer. Soc. of Agronomy. Wisconsin.
Nakai, Y., 1981: D genome donors forAegilops cylindrica (CCDD) andTriticum aestivum (AABBDD) deduced from esterase isozyme analysis. — Theor. Appl. Genet.60: 11–16.
—, 1982: D genome donors forAegilops crassa (DDMcrMcr, DDD2D2McrMcr) andAe. vavilovii (DDMcrMcrSpSp) deduced from esterase analysis by isoelectric focusing. — Japan J. Genet.57: 349–360.
Odintsova, T. I., Egorov, T. A., 1989: Isolation and characterization of gliadins fromAegilops squarrosa seeds. — Biokhimiya54: 267–336.
Platt, S. G., Kasarda, D. D., Qualset, C. O., 1974: Varietal relationships of the alphagliadin proteins in wheat. — J. Sci. Food Agric.25: 1555–1561.
Reeves, C. D., Okita, T. W., 1987: Analyses of alpha/beta-type gliadin genes from diploid and hexaploid wheats. — Gene52: 257–266.
Showalter, D. B., Sommer, S. S., 1989: The generation of radiolabelled DNA and RNA probes with polymerase chain reaction. — Anal. Biochem.177: 90–94.
Southern, E., 1975: Detection of specific sequences among DNA fragments separated by gel electrophoresis. — J. Mol. Biol.98: 503–509.
Wahl, G. M., Stern, M., Stark, G. R., 1979: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethal-paper and rapid hybridization by using dextran sulfate. — Proc. Natl. Acad. Sci. U.S.A.76: 3683–3685.
Witcombe, J. R., 1983: A guide to the species ofAegilops L. Their taxonomy, morphology and distribution. — AGP: Int. Board Plant Genet. Resources 83/77.
Woychik, J. H., Boundy, J. A., Dimler, R. J., 1961: Starch gel electrophoresis of wheat gluten proteins with concentrated urea. — Arch. Biochem. Biophys.94: 477–482.
Zhukovski, P. M., 1928: A critical-systematical survey of the species of the genusAegilops L. — Bull. App. Bot. Genet. Pl. Breed.18: 417–609.
Zohary, D., Feldman, M., 1962: Hybridisation between amphiploids and the evolution of polyploids in the wheat (Aegilops-Triticum) group. — Evolution16: 44–61.
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Masci, S., D'Ovidio, R., Lafiandra, D. et al. Electrophoretic and molecular analysis of alpha-gliadins inAegilops species (Poaceae) belonging to the D genome cluster and in their putative progenitors. Pl Syst Evol 179, 115–128 (1992). https://doi.org/10.1007/BF00938024
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DOI: https://doi.org/10.1007/BF00938024