Summary
Primary fixation of the frog oocyte with glutaraldehyde, compared to osmium tetroxide, alters the appearance of components involved in the morphogenesis of annulate lamellae. With glutaraldehyde, the outer layer of the nuclear envelope is connected with membranous laminae of variable length. Rounded blebs of the outer layer of the nuclear envelope are infrequently observed. Further, rather than clusters or rows of cytoplasmic vesicles as are observed in osmium tetroxide-fixed cells; numerous and long, smooth-surfaced lamellae are present in the ooplasm after glutaraldehyde fixation. The long membranous laminae then become concentrated in several ooplasmic packets. This is followed by the progressive alignment or orientation of the laminae within the packet. Eventually, those aligned and formerly smooth-surfaced lamellae are converted into annulate lamellae.
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This study was supported by research grants (HD-00699, GM-09229) and a Career Development Award from the National Institutes of Health, U.S. Public Health Service.
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Kessel, R.G. The effect of glutaraldehyde fixation on the elucidation of the morphogenesis of annulate lamellae in oocytes ofRana pipiens . Z.Zellforsch 94, 454–461 (1969). https://doi.org/10.1007/BF00936052
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DOI: https://doi.org/10.1007/BF00936052