Abstract
For systemic therapy against trophozoites of the skin-inhabiting stage ofIchthyophthirius multifiliis in ornamental fish, the latter were fed medicated food flakes containing malachite green once daily for 1–11 days ad libitum. Naturally or artificially infected cardinal tetras (Paracheirodon axelrodi), blue gouramis (Trichogaster trichopterus), or clown loach (Botia macracantha) were used in the trials. The fish were maintained in aerated 12.5- or 60-1 aquaria at 23°C. Ultrastructural investigations (scanning and transmission electron microscopy) revealed clear deleterious effects of malachite green on the parasitic stages. Following the initial application, the inner membrane of the mitochondria was destroyed. In fish fed for 2 days, aggregation of the mucocysts and polymerization the microtubules within the macronucleus occurred. Finally, the trophozoite's membrane was completely destroyed. In fish fed for 4 days, the medicated food killed all trophozoites ofI. multifiliis. Sensitive ornamental fish (e.g.,P. axelrodi) showed no adverse effects after they had been fed with only the medicated food flakes for 2 months. Therefore, the oral administration of malachite green using this newly developed medicated food considerably reduces the risk of toxic effects on the fish hosts, which are sometimes caused by malachite green following its application by immersion therapy. The feeding of flakes medicated with malachite green provides an easy-to-handle and highly effective treatment ofI. multifiliis in ornamental fish.
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Abbreviations
- C :
-
cilium
- CD :
-
core of electron-dense material
- CY :
-
cytoplasm
- DV :
-
digestive vacuole
- E :
-
erythrocyte
- EG :
-
electrondense granules
- EL :
-
electron-lucent hole
- F :
-
fold
- FL :
-
focal lysis of the cytoplasm
- HA :
-
heterochromatin aggregation
- HG :
-
heterophilic granulocyte
- I :
-
interruption of the outer membrane
- IM :
-
inner alvcolar membrane
- K :
-
knobbed end of a cilium
- LC :
-
lymphocyte
- MA :
-
macrophage
- MC :
-
monocyte
- MI :
-
mitochondrion
- MN :
-
macronucleus
- MT :
-
microtubules
- MU :
-
mucocyst
- N :
-
nucleus
- NG :
-
neutrophilic granulocyte
- OB :
-
ovoid body
- OM :
-
outer alveolar membrane
- PP :
-
pseudopodial protrusion
- PS :
-
parasomal sac
- R :
-
ribosome
- T :
-
trophozoite
- V :
-
vacuolization caused by treatment
- VA :
-
vacuole
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Schmahl, G., Ruider, S., Mehlhorn, H. et al. Treatment of fish parasites. Parasitol Res 78, 183–192 (1992). https://doi.org/10.1007/BF00931725
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DOI: https://doi.org/10.1007/BF00931725