Abstract
AchatininH (ATNH)is a lectin, isolated from the hemolymph ofAchatina fulica snail, which has been shown to have narrow specificity towards 9-O-acetyl sialic acid. Usually ATNH does not agglutinate normal human erythrocytes, however, it is capable of agglutinating erythrocytes of patients suffering from acute lymphocytic and acute myelogenous leukemia. Determination of binding constants, numbers of binding sites and lectin overlay experiments using patients' erythrocytes ghost, have suggested that some alterations in erythrocyte cell surface sialoglycoproteins or more precisely appearance of some O-acetylated sialoglycoprotein as a result of pathological transformations has caused this change in the binding of ATNH.
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Abbreviations
- ATNH :
-
AchatininH
- 9-OAc-NeuAc:
-
9-O-acetyl N-acetyl neuraminic acid
- BSM:
-
Bovine submaxillary mucin
- TBS:
-
Tris-buffered saline
- SDS-PAGE:
-
Sodium dodecyl sulphate polyacrylamide gel electrophoresis
- BSA:
-
Bovine serum albumin
- HA:
-
Hemagglutination assay
- ALL:
-
Acute lymphocytic leukemia
- AML:
-
Acute myelogenuos leukemia
- NP 40:
-
Nonidet 40
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Sen, G., Chowdhury, M. & Mandal, C. O-acetylated sialic acid as a distinct marker for differentiation between several leukemia erythrocytes. Mol Cell Biochem 136, 65–70 (1994). https://doi.org/10.1007/BF00931606
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DOI: https://doi.org/10.1007/BF00931606