Skip to main content
SpringerLink
Log in

Identification of human chorionic gonadotropin (HCG) secreting cells and other cell types using antibody to HCG and a new monoclonal antibody (mABlu-5) in cultures of human placental villi

  • Originals
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Summary

We have identified cells which secrete human chorionic gonadotropin (HCG) of cultures if first trimester placental villi. As a first step, we identified epithelial cells using a new monoclonal antibody. We then added HCG antibodies to the cultured cells. We found that syncytiotrophoblast (and not cytotrophoblast), Hofbauer cells and some mesenchymal cells stained with HCG antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aladjem S, Lueck J (1981) Morphologic characteristics of the normal tem human trophoblast maintained in prolonged in vitro cultures. Br J Obstet Gynaecol 88:287–293

    PubMed  Google Scholar 

  2. Aladjem S, Lueck J, Tsai A (1980) A method for prolonged in vitro culture of normal human trophoblast. Fed Proc Am Soc Exp Biol [Abstr. 1261] 39:506

    Google Scholar 

  3. Beck T, Schweikhart G, Stolz E (1986) Immunohistochemical location of HPL, SP1 and beta-HCG in normal placentas of varying gestational age. Arch Gynecol 239:63–74

    PubMed  Google Scholar 

  4. Boyd JD, Hamilton WJ (1970) The human placenta, Heffer and Sons Ltd, Cambridge, pp 143–174

    Google Scholar 

  5. Chung HK, McLimans WF, Horoszewicz J, Hreshchyshyn M (1969) In vitro studies of human trophoblast. Am J Obstet Gynecol 104:945–952

    PubMed  Google Scholar 

  6. Cotte C, Easty GC, Neville AM, Monaghan P (1980) Preparation of highly purified cytotrophoblast from human placenta with subsequent modulation to from syncytiotrophoblast in nonolayer cultures. In Vitro 16:639–646

    PubMed  Google Scholar 

  7. De Ikonicoff LK, Cedard L (1973) Localization of human chorionic gonadotropic and somatommatropic hormones by the peroxidase immunohistoenzymologic method in villi and amniotic epithelium of human placentas (from six weeks to term). Am J Obstet Gynecol 116:1124–1132

    PubMed  Google Scholar 

  8. Foldes J, Kehaty T, Schwartz J (1973) Interpretation of the multinucleated giant cell in human trophoblast cultures. Gynecol Inyest 4:254–262

    Google Scholar 

  9. Fox H, Kharkongor FN (1970a) Morphology and enzyme histochemistry of cells derived from placental villi in tissue culture. J Pathol 101:267–276

    PubMed  Google Scholar 

  10. Fox H, Kharkongor FN (1970b) Immunofluorescent localization of chorionic gonadotropin in the placenta and in tissue cultures of human trophoblast. J Pathol 101:277–282

    PubMed  Google Scholar 

  11. Guesdon JL, Ternynck T, Avrameas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131–1139

    PubMed  Google Scholar 

  12. Hertig AT, Rock J (1941) Two human ova of the previllous stage having an ovulation age of about eleven and twelve days respectively. Contrib Embryol Carneg Inst 29:127–156

    Google Scholar 

  13. Kaufmann P, Stark J (1973) Semidünnschnitt—zytochemische und immunautoradiographische Befunde zum Hormonstoffwechsel der reifen menschlichen Plazenta. Verh Anat Ges 67:245

    Google Scholar 

  14. Kaufmann P, Stark J, Stegner HE (1977) The villous stroma of the human placenta. 1. The ultrastructure of fixed connective tissue cells. Cell Tissue Res 177:105–121

    PubMed  Google Scholar 

  15. Loke YW, Borland R (1970) Immunofluorescent localization of chorionic gonadotrophin in monolayer cultures of human trophoblast cells. Nature 228:561–562

    PubMed  Google Scholar 

  16. Midgley AR, Pierce GB (1962) Immunohistochemical localization of human chorionic gonadotropin. J Exp Med 115:289–294

    PubMed  Google Scholar 

  17. Roy PW, Ryan GE, Bransome Jr ED (1976) Primary culture and maintenance of steroid-secreting human placental monolayers. In Vitro 12:115–119

    PubMed  Google Scholar 

  18. Stromberg K, Azizkhan JC, Speeg Jr KV (1978) Isolation of functional human trophoblast cells and their partial characterization in primary cell culture. In Vitro 14:631–638

    PubMed  Google Scholar 

  19. Taylor PC, Hancock KW (1973) Viability of human trophoblast in vitro. J Obstet Gynecol Br Commonw 80:834–840

    Google Scholar 

  20. Thiede H (1960) Studies of the human trophoblast in tissue culture. I. Culture method and histochemical studies. Am J Obstet Gynecol 79:636–647

    PubMed  Google Scholar 

  21. Von Overbeck J, Stähli C, Gudat F, Carmann H, Lautenschlager C, Dürmüller U, Takacs B, Miggiano V, Staechelin Th, Heitz PhW (1985) Immunohistochemical characterization of an antiepithelial monoclonal antibody (mABlu-5). Virchows Arch A 407:1–12

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Basle, Switzerland

    M. Frauli & H. Ludwig

Authors
  1. M. Frauli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Ludwig
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Frauli, M., Ludwig, H. Identification of human chorionic gonadotropin (HCG) secreting cells and other cell types using antibody to HCG and a new monoclonal antibody (mABlu-5) in cultures of human placental villi. Arch Gynecol Obstet 241, 97–110 (1987). https://doi.org/10.1007/BF00931230

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00931230

Key words

Search

Navigation