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Fractionation of erytroblasts with affinitymediated modifications of their electrical properties using counter-current distribution

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Abstract

We have previously reported the possibility of modifying the electrical properties of cells by means of their interaction with a specific ligand carrying a polyelectrolyte (Anal Biochem 200: 280–285). This selective modification of receptorcontaining cells changed their partition in a charge-sensitive aqueous two-phase system. We here present the fractionation of electrically modified erythroblasts by the use of an automatic multiple-partition procedure, counter-current distribution. The cells were fractionated according to the degree of differentiation of erythroblasts as evaluated from the hemoglobin content as well as the relative activities of the two enzymes, 3-phosphoglycerate kinase and bisphospho-glycerate mutase.

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Authors and Affiliations

  1. Department of Biochemistry, Chemical Center, University of Lund, P.O. Box 124, S-221 00, Lund, Sweden

    Jesús Mendieta & Göte Johansson

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  1. Jesús Mendieta
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  2. Göte Johansson
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Mendieta, J., Johansson, G. Fractionation of erytroblasts with affinitymediated modifications of their electrical properties using counter-current distribution. Mol Cell Biochem 121, 93–98 (1993). https://doi.org/10.1007/BF00928704

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  • DOI: https://doi.org/10.1007/BF00928704

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