Abstract
It was found that chronic intoxication of rats with acetaldehyde results in a distinct, progressive increase of 5-3H-proline incorporation into collagen synthesized by liver. At the same time biosynthesis of other proline-containing (noncollagenous) proteins does not change significantly. On the other hand the collagen content in the rat liver did not increase in the early stage of acetaldehyde administration, but increased when acetaldehyde feeding was continued for 6 months. About 40% increase of total collagen content was found in livers of the intoxicated animals. All the investigated collagen types (I, III, IV and V) grew in the same degree. No changes in proportional relationships between collagens of different types were found.
Similar content being viewed by others
References
Bańkowski E, Pawlicka E, Jodczyk KJ: Prolyl hydroxylase activity as an index of liver damage induced by ethanol. Cell Biochem Function 5: 221–215, 1987
Pawlicka E, Bańkowski E, Palka J, Markiewicz W: Prolyl hydroxylase activity in liver and serum of rats and human subjects intoxicated by chronic administration of ethanol. Hepatogastroenterol 34: 243–245, 1987
Pawlicka E, Bańkowski E, Chrostek L, Szmitkowski M: An increase of collagen biosynthesis precedes other symptoms of ethanol-induced liver damage in rats. Drug Alcohol Dependence 22: 113–116, 1988
Bańkowski E, Pawlicka E: An increase of collagen biosynthesis in livers of rats submitted to chronic intoxication with ethanol. Mol Cell Biochem 85: 43–48, 1989
Bańkowski E, Pawlicka E: Liver collagen of rats submitted to chronic intoxication with ethanol. Hepatogastroenterol, in press
Mezey E, Potter JJ, Slusser RJ, Abdi W: Changes in hepatic collagen metabolism in rats produced by chronic ethanol feeding. Lab Invest 36: 206–214, 1977
Chen TSN, Leevy CM: Collagen biosynthesis in liver disease of the alcoholic. J Lab Clin Med 85: 103–112, 1975
Perier C, Gautier M, Baril M, Bayle JJ, Patouillard G, Frey J: Measurement of changes in amino acids related to total collagen in fibrotic human liver. Clin Physiol Biochem 2: 279–286, 1984
Feinman L, Lieber CS: Hepatic collagen metabolism: Effect of alcohol consumption in rats and baboons. Science 176: 795, 1972
Rojkind M, Martinez-Palomo A: Increase in type I and type III collagens in human alcoholic liver cirrhosis. Proc Natl Acad Sci USA 78: 539–543, 1976
Mann SW, Fuller GC, Rodil JV, Vidins EJ: Hepatic prolyl hydroxylase and collagen synthesis in patients with alcoholic liver disease. Gut 20: 825–832, 1979
Mezey E, Potter JJ, Iber FI, Maddrey WC: Hepatic collagen proline hydroxylase activity in alcohol hepatitis: Effect of α-penicilamine. J Lab Clin Med 93: 92–100, 1979
Matsuoka M, Tsukamoto H: Stimulation of hepatic lipocyte collagen production by Kupffer cell-derived transforming growth factor β: Implications for a pathogenetic role in alcoholic liver fibrogenesis. Hepatology 11: 599–605, 1990
French SW, Miyamoto K, Wong K, Jui L, Briere L: Role of the Ito cell in liver parenchymal fibrosis in rats fed alcohol and a high fat-low protein diet. Am J Pathol 132: 73–85, 1988
Tsukamoto H, Towner SJ, Ciofalo LM, French SW: Ethanol-induced liver fibrosis in rats. Hepatology 6: 814–822, 1986
Barnes Y, Houser S, Barile FA: Temporal effects of ethanol on growth, thymidine uptake, protein and collagen production in human foetal lung fibroblasts. Toxicin Vitro 4: 1–7, 1990
Thanasi NM, Rowkowski RJ, Sheehy J, Hart B, Absher M, Cutroneo KR: Non-selective decrease of collagen synthesis by cultured fetal lung fibroblasts after non-lethal doses of ethanol. Biochem Pharmacol 29: 2417–2424, 1980
Holt K, Bennett M, Chojker M: Acetaldehyde stimulates collagen and noncollagen protein production by human fibroblasts. Hepatology 4: 843–848, 1984
Brenner DA, Chojkier M: Acetaldehyde increases collagen gene transcription in cultured human fibroblasts. J Biol Chem 36: 17690–17695, 1987
Lieber CS: Hepatic metabolic and toxic effects of ethanol: 1991 update. Alcoholism: Clin Exp Res 15: 573–592, 1991
Carrington MJ, Fornieri C, Bartlet CP, Heale G, Levene CI: Biochemical and ultrastructural study of the effects on collagen synthesis in 3T6. J Pharm Pharmacol 37: 794–801, 1985
Peterkofsky B, Diegelmann R: Use of a mixture of proteinase-free collagenase for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry 10: 988–994, 1971
Prockop DJ, Udenfriend S: A specific method for the analysis of hydroxyproline in tissues and urine. Anal Biochem 1: 228–239, 1960
Schofield JD, Freeman JL, Jackson DS: The isolation and amino acid and carbohydrate composition of polymeric collagens prepared from various human tissues. Biochem J 124: 467–473, 1971
Steven FS: The effect of chelating agents on collagen interfibrillar matrix interactions in connective tissue. Biochim Biophys Acta 140: 522–528, 1967
Murata K, Motayama T, Kotake C: Collagen types in various layer of the human aorta and their changes with the atherosclerotic process. Atherosclerosis 60: 251–262, 1986
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680–685, 1970
Rojkind M, Perez-Tamayo R: Liver fibrosis. Int Rev Connect Tissue Res 10: 333–394, 1983
Hasumura Y, Minato Y, Nishimura M, Kaku Y, Tozuka S, Koyama W, Tanaka Y, Takeuchi J: Hepatic fibrosis in alcoholics: Morphologic characteristics, clinical diagnosis, and natural course. Pathobiol Hepatic Fibrosis 7: 13–24, 1985
Lieber CS: Mechanism of cell injury. Biochemical mechanisms of alcohol-induced hepatic injury. Alcohol and Alcoholism, Suppl 1: 283–290, 1991
Lieber CS: Mechanisms of fibrogenesis. Alcohol and fibrogenesis. Alcohol and Alcoholism, Suppl 1: 339–344, 1991
Rojkind M, Ponce-Noyola P: The extracellular matrix of the liver. Collagen Rel Res 2: 151–175, 1982
Moshage H, Casini A, Lieber CS: Acetaldehyde selectively stimulates collagen production in cultures rat liver fat-storing cells but not in hepatocytes. Hepatology 12: 511–518, 1990
Okazaki I, Feinman L, Lieber CS: Hepatic mammalian collagenase: Development of an assay and demonstration of reversal activity after ethanol consumption. Gastroenterology 73: 1236, 1977
Topel H: Biochemical basis of alcoholism: Statements and hypotheses of present research. Alcohol 2: 711–788, 1985
Lin RC, Smith RS, Lumeng L: Detection of a protein-aldehyde adduct in the liver of rats fed alcohol chronically. J Clin Invest 81: 615–619, 1988
Tuma DJ, Newman MR, Donohue TM, Sorrell MF: Covalent bindig of acetaldehyde to proteins: Participation of lysine residues. Alcohol: Clin Exp Res 11: 579–584, 1987
Jukkola A, Niemelia O: Covalent binding of acetaldehyde to type III collagen. Biochem Biophys Res Commun 159: 163–169, 1989
Nomura F, Lieber CS: Binding of acetaldehyde to rat liver microsomes: Enhancement after chronic alcohol consumption. Biochem Biophys Res Commun 100: 131–137, 1981
Behrens UJ, Hoerner M, Lasker JM, Lieber CS: Formation of acetaldehyde adducts with ethanol-inducible P450IIE1in vivo. Biochem Biophys Res Commun 154: 584–590, 1988
Buehler R, Hess M, von Wartburg JP: Immunohistochemical localization of human liver alcohol dehydrogenase in liver tissue, cultured fibroblasts, and hela cells. Am J Pathol 108: 89–99, 1982
Tsutsumi M, Shimizu M, Lasker JM, Lieber CS: Intralobular distribution of ethanol-inducible cytochrome P-450IIE1 in liver (Abstr.). Hepatology 8: 1237, 1988
Lieber CS: Biochemical and molecular basis of alcohol-induced injury to liver and other tissues. New England J Med 319: 1639–1650, 1988
Von Wartburg JP, Buhler R: Biology of disease. Alcoholism and aldehydysm: New biomedical concepts. Lab Invest 50: 5–15, 1984
Eriksson CJP, Peachey YE: Absence of blood acetaldehyde differences between alcoholics and controls after ethanol ingestion. Drug Alcohol Depend 6: 35–36, 1980
Kato S, Murawaki Y, Hirayama C: Effects of ethanol feeding on hepatic collagen synthesis and degradation in rats. Res Commun Chem Pathol Pharmacol 47: 163–180, 1985
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bańkowski, E., Pawlicka, E. & Sobolewski, K. Liver collagen of rats submitted to chronic intoxication with acetaldehyde. Mol Cell Biochem 121, 37–43 (1993). https://doi.org/10.1007/BF00928698
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00928698