Abstract
The in vitro effect of Praziquantel (PZQ) onTaenia solium cysticerci was analyzed. The oxygen consumption rate of the parasites was inhibited and the release of proteins was enhanced, but no statistically significant differences were found between the control group and the experimental groups. The drug had a significant, dose-dependent negative effect on the evagination ability of the larvae; 50% effect was seen at concentrations of between 10−9 and 10−8 M PZQ. The drug also induced morphological disturbances in the tegument of the worm and of the bladder wall. Finally, a very drastic effect was the induction of spastic paralysis in evaginated cysticerci at high drug doses and of flaccid paralysis at lower PZQ concentrations. The dose inducing these effects was various orders of magnitude lower than that inhibiting the evagination ability of intact cysts. Moreover, the latter effect was reversible after had been washed out the drug and the parasites had been cultured. We suggest that PZQ alters the Ca2+ flux inT. solium as it does in other helminths. Furthermore, we corroborated the protective role of the bladder for the invaginated worm. Finally, we think that in vivo the drug must act synergistically with the immune system components so as to eliminate the parasite.
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References
Andrews P, Thomas H (1979) The effect of Praziquantel onHymenolepis diminuta in vitro. Z Parasitenkd 30:391–400
Andrews P, Thomas H, Pohlke R, Seubert J (1983) Praziquantel. Med Res Rev 3:147–200
Becker B, Mehlhorn H, Andrews P, Thomas H (1980a) Scanning and transmission electron microscope studies on the efficacy of Praziquantel onHymenolepis nana (Cestoda) in vitro. Z Parasitenkd 61:121–133
Becker B, Mehlhorn H, Andrews P, Thomas H, Eckert J (1980b) Light and electron microscopic studies on the effect of Praziquantel onSchistosoma mansoni, Dicrocoelium dendriticum andFasciola hepatica (Trematoda) in vitro. Z Parasitenkd 63:113–128
Becker B, Mehlhorn H, Andrews P, Thomas H (1981) Ultrastructural investigations on the effect of Praziquantel on the tegument of five species of cestodes. Z Parasitenkd 64:257–269
Brindley P, Sher A (1987) The chemotherapy effect of Praziquantel gainstSchistosoma mansoni is dependent on host antibody response. J Immunol 139:215–220
Cañedo L, Laclette JP, Morales E (1982) Evagination of the metacestode ofTaenia solium. In: Flisser A, Willms K, Laclette JP, Larralde C, Ridaura C, Beltran F (eds) Cysticercosis: present state of knowledge and perspectives Academic Press, New York, pp 363–373
Chavasse C, Brown M, Bell D (1979)Schistosoma mansoni: activity responses in vitro to Praziquantel. Z Parasitenkd 58:169–174
Coles G (1979) The effect of Praziquantel onSchistosoma mansoni. J Helminthol 53:31–33
Correa D, Laclette JP, Rodriguez-del-Rosal E, Merchant M, Flisser A (1987) Heterogeneity ofTaenia solium cysticerci obtained from different naturally infected pigs. J Parasitol 73:443–445
Earnest MP, Reller LB, Filley CM, Grek A (1987) Neurocysticercosis in the United States: 35 cases and a review. Rev Infect Dis 9:961–979
Fetterer R, Pax R, Bennett J (1980) Praziquantel, potassium and 2,4-dinitrophenol: analysis of their action on the musculature ofSchistosoma mansoni. Eur J Pharmacol 64:31–38
Flisser A, Delgado V, McLaren D (1989)Schistosoma mansoni: enhanced efficacy of Praziquantel treatment in immune mice. Parasite Immunol 11:319–328
Flisser A, Gonzalez D, Rodriguez-Carbajal M, Shkurovich M, Correa D, Cohen S, Delgado M, Madrazo M, Rodriguez-del-Rosal E, Fernandez B, Aluja A (1990) Praziquantel treatment of brain and muscle porcineTaenia solium cysticercosis. 1. Radiological, physiological and histopathological studies. Parasite Res 76:263–269
Gönnert R, Andrews P (1977) Praziquantel a new broad-spectrum antischistosomal agent. Z Parasitenkd 52:129–150
Groll EW (1982) Chemotherapy of human cysticercosis with Praziquantel. In: Flisser A, Willms K, Laclette JP, Larralde C, Ridaura C, Beltran F (eds) Cysticercosis: present state of knowledge and perspectives Academic Press, New York, pp 207–218
Harder A, Andrews P, Thomas H (1987) Chloropromazine, other amphiphilic cationic cationic drugs and Praziquantel: effects on carbohydrate metabolism ofSchistosoma mansoni. Parasitol Res 73:245–249
Harnett W, Kusel J (1986) Increased exposure of parasite antigens at the surface of adult maleSchistosoma mansoni exposed to Praziquantel in vitro. Parasitology 93:401–405
Lehninger A (1982) Principles of biochemistry. Worth, New York
Mehlhorn H, Becker B, Andrews P, Thomas H, Frenckel K (1981) In vivo and in vitro experiments on the effects of Praziquantel onSchistosoma mansoni. Arzneimittelforschung/Drug Res 31:544–554
Nechay B, Hilman G, Dotson M (1980) Properties and drug sensitivity of adenosine triphosphatases fromSchistosoma mansoni. J Parasitol 66:596–600
Pax R, Bennett J, Fetterer J (1978) A benzodiazepine and Praziquantel: effects on musculature ofSchistosoma mansoni andSchistosoma japonicum. Naunyn-Schmiedeberg's Arch Pharmacol 304:309–315
Pool P (1985) The effect of Praziquantel on the pseudophyllidean cestodeBothriocephalus acheilognathi in vitro. Z Parasitenkd 71:603–608
Prichard RK, Bachmann R, Hutchinson GW, Köhler P (1982) The effect of Praziquantel on calcium inHymenolepis diminuta. Mol Biochem Parasitol 5:297–308
Sabah A, Fletcher C, Webbe G, Doenhoff M (1985)Schistosoma mansoni: reduced efficacy of chemotherapy in infected T-cell deprived mice. Exp Parasitol 60:348–354
Schmahl G, Mehlhorn H (1985) Treatment of fish parasites. 1. Praziquantel effective against Monogenea (Dactylogyrus vastator, Dactylogyrus extensus, Diplozoon paradoxum). Z Parasitenkd 71:727–737
Shaw M, Erasmus D (1988)Schistosoma mansoni: Praziquantel-induced changes to female reproductive system. Exp Parasitol 65:31–42
Sotelo J, Torres B, Rubio-Donnadieu F, Escobedo F, Rodriguez-Carbajal J (1985) Praziquantel in the treatment of neurocysticercosis: long-term follow-up. Neurology 35:752–755
Terada M, Isshi A, Fujiu H, Sano M (1982) Studies on chemotherapy of parasitic helminths. IX. Effects of Praziquantel on the motility of various parasitic helminths and isolated tissues. Experientia 38:549–553
Thomas H, Gönnert R (1977) The efficacy of Praziquantel against cestodes in animals. Z Parasitenkd 52:117–127
Thomas H, Andrews P, Mehlhorn H (1982) New results on the effect of Praziquantel in experimental cysticercosis. Am J Trop Med Hyg 31:803–810
Wolde Musie E, Vande Waa J, Pax RA, Fetterer R, Bennett JL (1982)Schistosoma mansoni: calcium efflux and effects of calcium-free media responses of the adult male musculature to Praziquantel and other agents inducing contraction. Exp Parasitol 53:270–278
Xiao S, Friedman P, Catto B, Webster L (1984) Praziquantel-induced vesicle formation in the tegument of maleSchistosoma mansoni is calcium dependent. J Parasitol 70:177–179
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Garcia-Dominguez, C., Correa, D., Rabiela, M.T. et al. Praziquantel treatment of muscleTaenia solium cysticercosis. Parasitol Res 77, 691–696 (1991). https://doi.org/10.1007/BF00928684
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DOI: https://doi.org/10.1007/BF00928684