Abstract
Constituent glycoconjugates of promastigotes of 14 differentLeishmania strains from 6 different species were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently stained with 14125I-labelled lectins of different specificities. Autoradiography of the gels revealed lectinspecific glycoproteins and other glycoconjugates of known molecular weight. Similarities and differences in antigens and glycoproteins present in the strains are described. The possibility of identification or characterisation ofLeishmania species from their electrophoretic behaviour and lectin-binding patterns is unlikely, but these results should be an aid to purification of glycoconjugates from particular strains by lectin-affinity chromatography.
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Rossell, R.J., Stevens, A.F., Miles, M.A. et al. A comparison of the lectin-binding properties of glycoconjugates from a range ofLeishmania species. Parasitol Res 76, 294–300 (1990). https://doi.org/10.1007/BF00928182
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DOI: https://doi.org/10.1007/BF00928182