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Enhanced adhesion of human mononuclear cells to nonenzymatically glycosylated collagen I

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Abstract

Nonenzymatic glycosylation of extracellular matrix components may contribute to altered interaction of cells with the matrix. We have examined the interaction of mononuclear cells with early glycosylated collagen I. Significantly more cells attached to glycosylated collagen compared to normal collagen. Radioiodinated glycosylated collagen I specifically bound to mononuclear cells in a time and concentration dependent manner with a Kd of 2.45×10−9 M. Maximum binding was observed in the presence of Mn++ ions. The iodinated ligand bound to mononuclear cell membrane immobilized on nitrocellulose disks and the interaction was found to be saturable. These results suggested an alteration in the interaction of human blood mononuclear cells with collagen I, when it gets glycosylated non enzymatically and also indicate that early glycosylated collagen interacts with mononuclear cells through specific, high affinity cell surface molecules. (Mol Cell Biochem148: 115–121, 1995)

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  1. Department of Biochemistry, University of Kerala, Kariavattom, 695581, Trivandrum, India

    Rajesh P. Menon & P. R. Sudhakaran

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  1. Rajesh P. Menon
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  2. P. R. Sudhakaran
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Menon, R.P., Sudhakaran, P.R. Enhanced adhesion of human mononuclear cells to nonenzymatically glycosylated collagen I. Mol Cell Biochem 148, 115–121 (1995). https://doi.org/10.1007/BF00928148

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