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The effects of exogenous histamine in isolated rat hearts

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Abstract

The role of histamine in cardiac physiology and pathophysiology is not clarified, but is dependent on species. The effects of exogenous histamine in Langendorff-perfused rat hearts were investigated. 1 mM, 100, 10, 1 and 0.1 μM of histamine (n=7 each) as 15 min infusions were employed in a dose-response study, and compared to control perfused hearts (n=7). In another experimental series, 100 μM histamine (n=15) was added during reperfusion after 25 min global ischemia, and compared to control ischemia-reperfusion (n=15). The maximal response to histamine in the dose-response study (100 μM) was an increase of left ventricular developed pressure to 126±8% of initial value (mean±SEM, p<0.04), and increase of coronary flow to 152+6% (p<0.02) after 5 min infusion. 100 μM histamine did not significantly influence heart rate or rhythm. The lowest concentration (0.1 μM) did not have effects cardiac performance. Reperfusion with histamine for 2 min after ischemia reduced left ventricular developed pressure to 68±10% of initial value versus 116+17% in ischemic controls (p<0.05), and increased left ventricular end-diastolic pressure to 24±8 mmHg compared to 6±2 mmHg in controls (p<0.04). Left ventricular pressures were similar in hearts reperfused with histamine and in ischemic controls for the rest of the observation. Coronary flow increased during reperfusion in hearts given histamine. Histamine had a dose-dependent positive inotropic and vasodilatory effect in isolated rat hearts. Exogenous histamine had only minor effects on post-ischemic cardiac function.

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References

  1. McNeill JH: Histamine and the heart. Can J Physiol Pharmacol 62: 720–726, 1984

    Google Scholar 

  2. Levi R, Owen DAA, Trzeciakowski J: Actions of histamine in the heart and vasculature. In: C.R. Ganellin and M.E. Pason (eds). Pharmacology of histamine receptors. Wright PSG, 1982, pp 236–297

  3. Levi R, Rubin LE, Gross SS: Histamine in cardiovascular function and dysfunction: Recent developments. In: B. Uvnäs (ed.). Histamine and histamine antagonists. Springer Verlag, Berlin, 1991, pp. 347–374

    Google Scholar 

  4. Valen G, Kaszaki J, Szabo I, Nagy S, Vaage J: Toxic oxygen metabolites and ischemia-reperfusion increase histamine synthesis and release in the isolated rat heart. J Mol Cell Cardiol 25: 31–40, 1993

    Google Scholar 

  5. Masini E, Planchenault J, Pezzardi F, Gautier P, Gagnol J: Histamine release during an experimental coronary thrombosis in awake dog. Agents Actions 16: 227–230, 1985

    Google Scholar 

  6. Levi R, Wolff A, Robertson DA, Graver L: IgE-mediated hypersensitivity and ischemia as causes of endogenous cardiac histamine release. Adv Biosci 51: 305–307, 1985

    Google Scholar 

  7. Masini E, Bianchi S, Gambassi F, Palmerani B, Pistelli A, Carlomagno L, Mannaioni PF: Ischemia reperfusion injury and histamine release in isolated and perfused guinea pig heart: Pharmacological interventions. Agents Actions 30: 198–201, 1990

    Google Scholar 

  8. Masini E, Gambassi F, Palmerani B, Carlomagno L, Mannaioni PF: Ischemia-reperfusion injury and histamine release in isolated guinea pig heart: The role of free radicals. Agents Actions 27: 154–157, 1989

    Google Scholar 

  9. Valen G, Kaszaki J, Nagy S, Vaage J: Open heart surgery increases histamine in arterial and coronary sinus blood. Agents Actions 41: 11–16, 1994

    Google Scholar 

  10. Rai V, Bhatt PS, Singh RH, Vaish SK, Udupa KN: Histamine and histaminase changes in arterial hypertension and myocardial infarction. Indian Heart J 28: 115–117, 1976

    Google Scholar 

  11. Zaca F, Benassi MS, Ghinelli M, Trianni M, Vaccarino R, Malavolta E, Muratori M, Lenzi S: Myocardial infarction and histamine release. Agents Actions 18: 258–261, 1986

    Google Scholar 

  12. Ginsburg R, Bristow MR, Kantrowitz N, Baim DS, Harrison DC: Histamine provocation of clinical coronary artery spasm: Implications concerning pathogenesis of variant angina pectoris. Am Heart J 102: 819–822, 1981

    Google Scholar 

  13. Okumura K, Yasue H, Matsuyama K, Matsuyama K, Morikami Y, Ogawa H, Obata K: Effect of H1 receptor stimulation on coronary artery diameter in patients with variant angina: Comparison with effect of acetylcholine. J Am Coll Cardiol 17: 338–345, 1991

    Google Scholar 

  14. Dai S: Ventricular histamine concentrations and arrhythmias during acute myocardial ischemia. Agents Actions 29: 66–71, 1987

    Google Scholar 

  15. Dai S: Effects of SK&F 93479 on experimentally induced ventricular arrhythmias in dogs, rats and mice. Agents Actions 15: 131–136, 1984

    Google Scholar 

  16. Dai S: Effects of ranitidine and cimetidine on experimentally induced ventricular arrhythmias in anaesthetized rats. Agents Actions 17: 460–465, 1985

    Google Scholar 

  17. Valen G, Kaszaki J, Szabo I, Nagy S, Vaage J: Histamine release and its effects in ischaemia-reperfusion injury of the isolated rat heart. Acta Physiol Scand 150: 413–424, 1994

    Google Scholar 

  18. Reilly MA, Schayer RW: Further studies on histamine catabolismin vivo. Br J Pharmacol 43; 349–358, 1971

    Google Scholar 

  19. Maslinski C, Fogel WA: Catabolism of histamine. In: B. Uvnäs (ed.). Handbook Exp Pharmacol 97, Springer Verlag 1991, pp 165–190

  20. Perin A, Sessa A, Desidero MA: Polyamine levels and diamine oxidase activity in hypertrophic heart of spontaneously hypertensive rats treated with isoproterenol. Biochim Biophys Acta 755: 344–351, 1983

    Google Scholar 

  21. Dai S: A study of the actions of histamine on the isolated rat heart. Clin Exp Pharmacol Physiol 3: 359–367, 1976

    Google Scholar 

  22. Akar F, Ercan ZS, Turker RK: A marked H1-receptor-mediated vasodilator effect of histamine in the isolated perfused rat heart. Eur J Pharmacol 97: 265–269, 1984

    Google Scholar 

  23. Toda N: Mechanism of histamine actions in human coronary arteries. Circ Res 61: 280–286, 1987

    Google Scholar 

  24. Csete K, Auclair M-C, Lechat P: Chronotropic effect of histamine on cultured neonatal rat heart cells. Eur J Pharmacol 58: 117–123, 1979

    Google Scholar 

  25. McCall D, Lui CY: The effects of histamine on contraction frequency, sodium influx, and cyclic AMP in cultured rat heart cells. Circ Res 59: 668–675, 1986

    Google Scholar 

  26. Frkovic A, Skrobonja A, Atanakovic D: The chronotropic effect of histamine, prometasin and cimetidine on rat heart. Acta Physiol Pharmacol Bulgarica 14: 43–47, 1988

    Google Scholar 

  27. Cameron JS, Swigart CR, Shin GS, Katz D, Bassett AL: Enhanced susceptibility to histamine-induced cardiac arrhythmias in spontaneously hypertensive rats. J Cardiovasc Pharmacol 15: 626–632, 1990

    Google Scholar 

  28. Beaven MA: Radioenzymatic assays in biological fluids. In: B. Uvnäs (ed.). Handbook Exp Pharmacol 97, Springer Verlag 1991 pp 49–58

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Authors and Affiliations

  1. Department of Surgery, University of Tromsø, Box 60500, S-17176, Tromsø, Norway

    Guro Valen, Tove Skjelbakken & Jarle Vaage

  2. Department of Thoracic Surgery, Karolinska Hospital, Box 60500, S-17176, Stockholm, Sweden

    Guro Valen & Jarle Vaage

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  1. Guro Valen
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  2. Tove Skjelbakken
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  3. Jarle Vaage
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Valen, G., Skjelbakken, T. & Vaage, J. The effects of exogenous histamine in isolated rat hearts. Mol Cell Biochem 146, 55–61 (1995). https://doi.org/10.1007/BF00926882

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  • DOI: https://doi.org/10.1007/BF00926882

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