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Effects of iloprost, a PGI2 derivative, on ischemic myocardial energy and carbohydrate metabolism in dogs

  • Cellular Function and Metabolism
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Abstract

Effects of iloprost, which is a stable prostacyclin analogue, on the ischemic myocardium were examined in the open-chest dog heart, in terms of biochemical parameters. Ischemia was initiated by ligating the left anterior descending coronary artery. When the coronary artery was ligated for 3 min, the levels or glycogen, fructose-1,6-diphosphate (FDP), adenosine triphosphate and creatinephosphate decreased, and the levels of glucose-6-phosphate (G6P), fructose-6-phosphate (F6P), lactate, adenosine diphosphate and adenosine monophosphate increased. During ischemia, therefore, energy charge potential was significantly decreased from 0.89±0.01 to 0.82±0.01, and ([G6P]+[F6P])/[FDP] and [lactate]/[pyruvate] ratios were significantly increased from 1.75±0.30 to 29.05±5.70 and 13±3 to 393±112, respectively. Iloprost (0.1, 0.3, or 1 μg·kg−1) was injected intravenously 5 min before the onset of ischemia. Iloprost (0.1, 0.3, and 1 μg·kg−1) reduced the ischemia-induced decrease in energy charge potential to 94, 74, and 86%, respectively, the increase in ([G6P]+[F6P]/[FDP] to 38, 29, 32%, respectively, and the increase in [lactate]/[pyruvate] to 67, 45, 65%, respectively. These results suggest that iloprost lessens the myocardial metabolic derangements produced by ischemia, and the most potent effect was obtained at the dose of 0.3 μg·kg−1.

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Authors and Affiliations

  1. Department of Pharmacology, Asahikawa Medical College, 4-5 Nishikagura, Asahikawa 078, Japan

    Kazuo Ichihara, Kiminobu Yamamoto & Yasushi Abiko

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  1. Kazuo Ichihara
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  2. Kiminobu Yamamoto
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  3. Yasushi Abiko
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Ichihara, K., Yamamoto, K. & Abiko, Y. Effects of iloprost, a PGI2 derivative, on ischemic myocardial energy and carbohydrate metabolism in dogs. Mol Cell Biochem 119, 133–141 (1993). https://doi.org/10.1007/BF00926864

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