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Ca2+ inhibition of brush border alkaline phosphatase activity inHymenolepis diminuta

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Abstract

The brush border membrane ofHymenolepis diminuta contains several Ca2+-dependent enzymes. Following our isolation of a Ca2+-dependent modulator protein we examined the kinetic properties of the brush border marker alkaline phosphatase from fractionated and crude tegument. We show that this enzyme is inhibited by Ca2+ concentrations approaching those in the calcareous corpuscles ofH. diminuta.

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Abbreviations

CT:

crude tegument

FT:

fractionated tegument

EGTA:

ethylene glycol bis (amino-ethyl-ether) tetra acetic acid

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Authors and Affiliations

  1. Department of Biology, Oxford Polytechnic, Headington, OX3 OBP, Oxford, UK

    Jayne B. Hipkiss

  2. Bath College of Higher Education, Newton Park, Newton St. Loe, BA2 9BN, Bath, UK

    Christopher J. Branford White

Authors
  1. Jayne B. Hipkiss
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  2. Christopher J. Branford White
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Hipkiss, J.B., Branford White, C.J. Ca2+ inhibition of brush border alkaline phosphatase activity inHymenolepis diminuta . Z. Parasitenkd. 71, 759–763 (1985). https://doi.org/10.1007/BF00926801

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  • DOI: https://doi.org/10.1007/BF00926801

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