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Immunocytochemical localization of gut-associated circulating anodic antigen inSchistosoma japonicum

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Abstract

The localization of the gut-associated circulating anodic antigen inSchistosoma japonicum adults was revealed by means of the immunofluorescence and a peroxidase-anti-peroxidase method with the electron microscope. The reaction sites were confined to amorphous material in the cecal lumen. The cecal lumen generally was infolded with lamellae. The antigenic material appeared to be secreted by the rough endoplasmic reticulum, probably through the Golgi apparatus, into the lumen. Observations of male and female worms showed that there was a clear difference between the sexes in antigen concentration. The thick epithelium of the female worm, with well-developed cisternae on the endoplasmic reticulum, produced a lot more antigen than the male. Positive staining with ruthernium red confirmed that the antigenic material was a negatively charged polysaccharide as had been previously reported.

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References

  • Deelder AM, Kornelis D, van Marck EAE, Eveleigh PC, van Egmond JG (1980)Schistosoma mansoni: characterization of two circulating polysaccharide antigens and the immunological response to these antigens in mouse, hamster, and human infections. Exp Parasitol 50:16–32

    PubMed  Google Scholar 

  • Gold R, Rosen FS, Weller TH (1969) A specific circulating antigen in hamsters infected withSchistosoma mansoni Detection of antigen in serum and urine, and correlation between antigenic concentration and worm burden. Am J Trop Med Hyg 18:545–552

    PubMed  Google Scholar 

  • Hirata M (1976) Circulating antigen in animals infected withSchistosoma japonicum 2. Appearance of circulating antigen in infected mice. Jpn J Parasitol 25:396–401

    Google Scholar 

  • Hirata M (1981) Female-dependency of circulating anodic antigen level inSchistosoma japonicum infection. Jpn J Parasitol 30:429–437

    Google Scholar 

  • Hirata M, Akusawa M (1975) Circulating antigen in animals infected withSchistosoma japonicum 1. Detection and characteristics of circulating antigen in infected rabbits. Jpn J Parasitol 24:250–254

    Google Scholar 

  • Komuro T, Yamamoto T (1970) Unusual cytoplasmic tubules demonstrated with ruthenium red in the chloride cell of the gill filaments and urinary tubules. J electronmicrose 19:194

    Google Scholar 

  • Lichtenberg F von, Bawden MP, Shealey SH (1974) Origin of circulating antigen from the schistosome gut. Am J Trop Med Hyg 23:1088–1091

    PubMed  Google Scholar 

  • Luft JH (1964) Electron microscopy of cell extraneous coats as revealed by ruthenium red staining. J Cell Biol 23:54A-55A

    Google Scholar 

  • Luft JH (1965) The fine structure of hyaline cartilage matrix following ruthenium red fixative and staining. J Cell Biol 27:61A

    Google Scholar 

  • Luft JH (1966) Fine structure of capillary and endocapillary layer as revealed by ruthenium red. Fed Proc 25:1773–1783

    PubMed  Google Scholar 

  • Nash TE (1974) Localization of the circulating antigen within the gut ofSchistosoma mansoni. Am J Trop Med Hyg 23:1085–1087

    PubMed  Google Scholar 

  • Nash TE, Nasir UD, Jeanloz RW (1977) Further purification and characterization of a circulating antigen in schistosomiasis. J Immunol 119:1627–1633

    PubMed  Google Scholar 

  • Nash TE, Prescott B, Neva FA (1974) The characteristics of the circulating antigen in schistosomiasis. J Immunol 112:1500–1507

    PubMed  Google Scholar 

  • Qian ZL, Deelder AM (1983)Schistosoma japonicum: immunological characterization and detection of circulating polysaccharide antigens from adult worms. Exp Parasitol 55:168–178

    PubMed  Google Scholar 

  • Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333

    PubMed  Google Scholar 

  • Tani E, Ametani T (1970) Substructure of microtubules in brain nerve cells as revealed by ruthenium red. J Cell Biol 46:159–165

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan

    T. Fujino & Y. Ishii

  2. Department of Parasitology, Kurume University School of Medicine, 830, Kurume, Japan

    M. Hirata & H. Tsutsumi

Authors
  1. T. Fujino
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  2. M. Hirata
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  3. Y. Ishii
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  4. H. Tsutsumi
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Fujino, T., Hirata, M., Ishii, Y. et al. Immunocytochemical localization of gut-associated circulating anodic antigen inSchistosoma japonicum . Z. Parasitenkd. 71, 739–745 (1985). https://doi.org/10.1007/BF00926799

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  • DOI: https://doi.org/10.1007/BF00926799

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