Abstract
Crithidia fasciculata was utilized as a prescreen to determine the antiprotozoal action of aminoglycoside antibiotics alone and in combination with surface-altering agents. Paromomycin was tested with the carrier ionophores nigericin and valinomycin, the channel ionophore gramicidin and the polyene antibiotics amphotericin B and nystatin. After exposure to the drugs in suspension, organisms were plated out to determine the survival ofC. fasciculata. Killing was time dependent for both the antibiotic and the ionophore. Paromomycin action was found to be potentiated by all the surface altering agents. The aminoglycosides kanamycin, gentamycin and streptomycin were studied alone and in combination with nigericin. Synergistic effects were demonstrated both with kanamycin and gentamycin in combination with nigericin. Streptomycin was ineffective both alone and with surface-altering agents.
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References
Arustamyan T (1967) Treatment with monomycin of patients with acute necrotizing cutaneous leishmaniasis. Vest Derm Vener 41:45–49 (in Russian), English summary: Trop Dis Bull 65 abst 526
El-On J, Pearlman E, Messer Y, Greenblatt C (1981) Experimental basis for the action of rifampicin in cutaneous leishmaniasis and its use for combined therapy with amphotericin B. 12th International Congress of Chemotherapy, Florence abst 1164
Even-Paz Z, Weinrauch L, Livshin R, El-On J, Greenblatt CL (1982) Rifampicin treatment of cutaneous leishmaniasis. Intl J Dermatol 21:110–112
Hughes DE, Schneider CA, Simpson L (1982) Isolation and characterization of drug resistant mutants ofCrithidia fasciculata. J Parasitol 68:642–649
Mates SM, Eisenberg ES, Mandel LJ, Patel L, Kaback HR, Miller MM (1982) Membrane potential and gentamycin uptake inStaphylococcus aureus. Proc Natl Acad Sci USA 79:6693–6697
Midgley M (1978) The transport of α-aminoisobutyrate intoCrithidia fasciculata. Biochem J 174:191–202
Neal RA (1968) The effect of antibiotics of the neomycin group on experimental cutaneous leishmaniasis. Ann Trop Med Parasitol 62:54–62
Peters W, Lainson R, Shaw JJ, Robinson BL, Franca Leao A (1981) Potentiating action of rifampicin and isoniazid againstLeishmania mexicana amazonesis. The Lancet i:1122–1123
Seqouia M, Pena P, Marin F, Marin-Lueng F (1981) In vitro activity of rifampicin againstLeishmania aethiopica. 12th International Congress of Chemotherapy, Florence, abst 1162
Shapiro A, Hutner SH, Katz L, Bacchi CJ (1981) Rapid in vitro prescreen for chelators as potential trypanocides based on growth ofCrithidia fasciculata. J Protozool 28:370–377
Wilhelm JM, Pettitt SE, Jessop JJ (1978a) Aminoglycoside antibiotics and eukaryotic protein synthesis: structure-function relationships in the simulation of misreading with a wheat embryo system. Biochemistry 17:1143–1149
Wilhelm JM, Pettitt SE, Jessop JJ (1978b) Aminoglycoside antibiotics and eukaryotic protein synthesis: stimulation of errors in the translation of natural messengers in extracts of cultured human cells. Biochemistry 17:1149–1153
Zilberstein D, Dwyer DM (1985) Proton motive force driven active transport ofd-glucose andl-proline in the protozoan parasiteLeishamnia Donovani. Cell Biology (in press)
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Millgram, M.A., Greenblatt, C.L. & Shlomai, J. Interaction of aminoglycosides and ionophores in the killing ofCrithidia fasciculata . Z. Parasitenkd. 71, 699–704 (1985). https://doi.org/10.1007/BF00926795
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DOI: https://doi.org/10.1007/BF00926795