Abstract
Triton X-100 extracts of erythrocytes infected withPlasmodium chabaudi andP. yoelii were analysed in crossed immunoelectrophoresis with rabbit antisera. The parasite origin of the antigens detected was assessed by metabolic radiolabelling of the parasites with35S-methionine. About 12 immunoprecipitates were obtained with both extracts and their homologous antiserum. Cross-tests showed that the two parasite strains were very similar antigenically. Species-specific antigens could, however, also be demonstrated. Two antigens, present on bothP. yoelii- andP. chabaudi-infected erythrocytes, were located on the surface of the host cell membrane as judged from125I-labellings with lactoperoxidase. Experiments with phenyl-Sepharose showed that most of the antigens detected were hydrophilic and none of them reacted with concanavalin A.
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Carlsson, J., Berzins, K. & Perlmann, P. Immunoelectrophoretic analysis of erythrocytic stages ofPlasmodium yoelii andP. chabaudi . Z. Parasitenkd. 70, 599–608 (1984). https://doi.org/10.1007/BF00926590
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DOI: https://doi.org/10.1007/BF00926590