Abstract
Purpurogallin (PPG) has been used as an additive to edible and non-edible oils or fats to retard oxidation. Its antioxidant mechanism is not known. We investigated the ability of PPG to scavenge exogenously generated hydroxyl radicals (·OH) using a sensitive high pressure liquid chromatographic (HPLC) method. ·OH was generated by photolysis of H2O2 (1.25–10 μmoles) with UV light and was trapped with salicylic acid (500 nmoles). Salicylic acid is hydroxylated to produce ·OH adduct products 2,3-and 2,5-dihydroxybenzoic acid (DHBA). H2O2 produced concentration-dependent ·OH as estimated by generation of 2,3- and 2,5-DHBA. PPG (100, 200, 300, 400, 500 and 600 nmoles) produced concentration-dependent decreases in ·OH adduct products (approximately 70% inhibition with 600 nmoles of PPG). It did not affect the peak of standard 2,3- and 2,5-DHBA indicating that the decrease in the adduct product generated by H2O2 is due to scavenging of ·OH. These results indicate that photolysis of H2O2 by UV light produces ·OH and that PPG scavenges ·OH.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Claus EP: Pharmacognosy, 3rd Edition, Lea and Febiger, Philadelphia, 1965, pp 231–233
Critchlow A, Haslam E, Haworth ED, Tinker PB, Waldron NM: The oxidation of some pyrogallol and purpurogallin derivatives. Tetrahedron 23: 2829–2847, 1967
Anderson WA: Hydroxylic aromatic compounds as additives for rubber based, composite solid propellants. U.S. Patent 4181545 assigned to United Technology Corporation, 1980
Wu TW, Wu J, Carey D, Zeng LH: Purpurogallin protects both ventriculat myocytes and aortic endothelial cells of rats against oxyradical damage. Biochem Cell Biol 70: 803–809, 1992
Wu TW, Zeng LH, Wu J, Carey D: Purpurogallin — a natural and effective hepatoprotectorin-vitro andin vivo. Biochem Cell Biol 69: 747–759, 1991
Sugiyama H,Fung KP, Wu TW: Purpurogallin as an antioxidant protector of human erythrocytes against lysis by peroxyl radicals. Life Sci 53: 39–43, 1993
Floyd RA, Watson JJ, Wong PK: Sensitive assay of hydroxyl free radical formation utilizing high pressure liquid chromatography with electrochemical detection of phenol and salicylate hydroxylation products. J Biochem Biophys Methods 10: 221–235, 1984
Floyd RA, Henderson R, Watson JJ, Wong PK: Use of salicylate with high pressure liquid chromatography and electrochemical detection (LCED) as a sensitive measure of hydroxyl free radical in adriamycin treated rats. J Free Rad Biol Med 2: 13–18, 1986
Grootveld M, Halliwell B: Aromatic hydroxylation as a potential measure of hydroxyl radical formationin vivo. Identification of hydroxylated derivatives of salicylate in human body fluids. Biochem J 237: 499–504, 1986
Onodera T, Ashraf M: Detection of hydroxyl radicals in the postichemic reperfused heart using salicylate as a trapping agent. J Mol Cell Cardiol 23: 365–370, 1991
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Prasad, K., Laxdal, V.A. Evaluation of hydroxyl radical-scavenging property of purpurogallin using high pressure liquid chromatography. Mol Cell Biochem 135, 153–158 (1994). https://doi.org/10.1007/BF00926518
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00926518