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Control of glycolytic enzyme binding: effect of changing enzyme substrate concentrations onin vivo enzyme distributions

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Abstract

The effect of changing concentrations of glycolytic intermediates on the binding of phosphofructokinase, aldolase and pyruvate kinase to cellular particulate matter was investigated. Concentrations of glycolytic intermediates were altered by adding 2mM iodoacetic acid (IAA) to an incubation medium containing tissues isolated from the channelled whelkBusycon canaliculatum. Iodoacetic acid inhibited glyceraldehyde 3-phosphate dehydrogenase activity causing a 100–400 fold increase in the concentration of fructose 1,6-bisphosphate as well as 3–20 fold increases in glucose 6-phosphate, fructose 6-phosphate, and dihydroxyacetone phosphate levels depending on the experimental protocol. Cellular pH values were not statistically different in the presence of IAA. Measurement of enzyme binding to particulate matter showed that the binding of phosphofructokinase, aldolase and pyruvate kinase was unaffected by iodoacetic acid under any experimental conditon. These results show that changes in the tissue concentrations of enzyme substrates and products do not regulate enzyme binding to particulate matter in the cell. (Mol Cell Biochem122: 1–7, 1993)

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Abbreviations

DHAP:

Dihydroxyacetone Phosphate

F6P:

Fructose 6-phosphate

FBP:

Fructose 1,6-bisphosphate

G3P:

Glyceraldehyde 3-phosphate

GAPDH:

Glyceraldehyde 3-phosphate Dehydrogenase

IAA:

Iodoacetic Acid

PEP:

Phosphoenolpyruvate

PFK:

Phosphofructokinase

PK:

Pyruvate Kinase

PYR:

Pyruvate

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Author notes

  1. Stephen P. J. Brooks

    Present address: Nutrition Research Division, Food Directorate, Health Protection Branch, Health and Welfare, Canada

Authors and Affiliations

  1. Department of Biology and Institute of Biochemistry, Carleton University, K1S 5B6, Ottawa, Ontario, Canada

    Stephen P. J. Brooks & Kenneth B. Storey

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  1. Stephen P. J. Brooks
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  2. Kenneth B. Storey
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Brooks, S.P.J., Storey, K.B. Control of glycolytic enzyme binding: effect of changing enzyme substrate concentrations onin vivo enzyme distributions. Mol Cell Biochem 122, 1–7 (1993). https://doi.org/10.1007/BF00925731

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  • DOI: https://doi.org/10.1007/BF00925731

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