Abstract
The peroxidase anti-peroxidase immunocytochemical staining method was used to identifyToxoplasma antigen in paraffin embedded sections of the brains of 22 mice congenitally infected with the parasite. IntactToxoplasma tissue cysts were readily demonstrated in the brain in all cases. In 4 of the 22 infected mice there was evidence of rupture of the cyst wall and/or presence of extra-cysticToxoplasma antigen. Further support for the extra-cystic location ofToxoplasma antigen was obtained by electron microscopy of reprocessed tissue which revealed endozoites in the area immediately surrounding a ruptured cyst. The possible implications of these findings in relation to the pathogenesis of congenital toxoplasmic meningo-encephalitis are discussed.
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Conley FK, Jenkins KA (1981) Immunohistological study of the anatomical relationship ofToxoplasma antigens to the inflammatory response in the brains of mice congenitally infected withToxoplasma gondii. Infect Immun 31:1184–1192
Desmonts G, Remington JS (1980) Direct agglutination test for diagnosis ofToxoplasma infection: method for increasing sensitivity and specificity. J Clin Microbiol 11:562–568
Dutton GN, Hay J, Hair DM, Ralston J (1986) Clinicopathological features of a congenital murine model of ocular toxoplasmosis. Graefe's Arch. Clin Exp Ophthal 224:256–264
Frenkel JK (1974) Pathology and pathogenesis of congenital toxoplasmosis. Bull NY Acad Med 50:182–191
Frenkel JK, Nelson BM, Arias-Stella J (1975) Immunosuppression and toxoplasmic encephalitis. Clinical and experimental aspects. Human Pathol 6:97–111
Graham DI, Hay J, Hutchison WM, Siim JC (1984) Encephalitis in mice with congenital ocular toxoplasmosis. J Path 142:265–277
Hay J, Hutchison WM, Lee WR, Siim JC (1981) Cataract in mice congenitally infected withToxoplasma gondii. Ann Trop Med Parasitol 75:455–457
Hay J, Dutton GN, Ralston J (1985a) Congenital toxoplasmic retinochoroiditis in the mouse-the use of the peroxidase antiperoxidase method to demonstrateToxoplasma antigen. Trans R Soc Trop Med Hyg 79:106–109
Hay J, Dutton GN, Hair DM (1985b) Blastogenic responses of splenic lymphocytes to toxoplasmal and retinal antigens and T- and B-cell mitogens in mice with congenital ocular toxoplasmosis. Ann Trop Med Parasitol 79:113–115
Huldt G (1966) Experimental toxoplasmosis. Studies on the multiplication and spread ofToxoplasma in experimentally infected rabbits. Acta Pathol Microbiol Scand [B] 67:401–423
Hutchison WM, Hay J, Lee WR, Siim JC (1982) A study of cataract in murine congenital toxoplasmosis. Ann Trop Med Parasitol 76:53–70
Johnson AM, Haynes WD, Leppard PJ, McDonald PJ, Neoh SH (1983) Ultrastructural and biochemical studies on the immunohistochemistry ofToxoplasma gondii antigens using monoclonal antibodies. Histochem 77:209–215
Kittas S, Kittas C, Paizi-Biza P, Henry L (1984) A histological and immunohistochemical study of the changes induced in the brains of white mice by infection withToxoplasma gondii. Brit J Exp Path 65:67–74
Lee WR, Hay J, Hutchison WM, Dutton GN, Siim JC (1983) A murine model of congenital toxoplasmic retinochoroiditis. Acta Ophthalmol 61:818–830
Reid WA, Thomson WD, Kay J (1984) Immunostaining of diffusable antigens after autolysis. J Path 142:A5
Sabin AB, Feldman HA (1948) Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoan parasite (Toxoplasma). Science 108:660–663
Sternberger LA (1979) Immunocytochemistry. 2nd Edition. John Wiley, New York
Wanko T, Jacobs L, Gavin MA (1962) Electron microscope study ofToxoplasma cysts in the mouse brain. J Protozool 9:235–242
Werner H, Masihi KN, Senk U (1981) LatentToxoplasma infection as a possible risk factor for CNS disorders. Zbl Bakt Hyg I Orig A 250:368–375
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Hay, J., Graham, D.I., Dutton, G.N. et al. The immunocytochemical demonstration ofToxoplasma antigen in the brains of congenitally infected mice. Z. Parasitenkd. 72, 609–615 (1986). https://doi.org/10.1007/BF00925482
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DOI: https://doi.org/10.1007/BF00925482