Abstract
The performance of complete post-mortem examinations of children with severe malaria has helped to explain the cause of death in cerebral malaria as well as show the global phenomenon of sequestration in tissues throughout the body, beyond the brain and eye. The pathology of the brain and other organs has been well described and shows a systemic disease with the most catastrophic features found in the brain (i.e., fatal cerebral edema).
This chapter describes the materials and methods needed to study the pathological features of tissues outside of the eye, including the brain and other organs. The bulk of these techniques are standard to pathology including gross examination, histology, special stains, and immunohistochemistry.
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Acknowledgments
The authors are grateful for the many profound contributions to this work by Terrie E Taylor (College of Osteopathic Medicine, Michigan State University, East Lansing, MI, USA; Blantyre Malaria Project, University of Malawi College of Medicine, Blantyre, Malawi.) and Malcolm E Molyneux (Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L35QA, UK).
Disclaimer: The content of this article represents the personal views of the authors and do not necessarily stand for the views of the authors’ employers or associated institutions. Where authors are found as personnel of the International Agency for Research on Cancer/World Health Organization, the authors alone handle the views expressed in this article, and these views do not necessarily stand for the decisions, policy, or views of the International Agency for Research on Cancer /World Health Organization.
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Milner, D.A., MacCormick, I.J.C. (2022). Histological Identification of Sequestered Parasitized Red Cells. In: Jensen, A.T.R., Hviid, L. (eds) Malaria Immunology. Methods in Molecular Biology, vol 2470. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2189-9_57
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DOI: https://doi.org/10.1007/978-1-0716-2189-9_57
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