Abstract
Immune-aggregate and thrombin-mediated [3H]serotonin release from human platelets are shown to be enhanced when platelets are preincubated with the antibacterial chymotrypsin-like cationic protein isolated from human granulocytes. The enhancement is dose dependent and inhibited by heating of the cationic protein. Release with chymotrypsin-like cationic protein alone was not observed, although the protein was shown to “micro-aggregate” platelets irreversibly by an ADP-dependent reaction. Platelet “macro-aggregation” induced by immune-aggregate was also enhanced by chymotrypsin-like cationic protein whereas platelet “macro-aggregation” induced by thrombin was inhibited competitively. Platelet “micro-aggregation” induced by chymotrypsin-like cationic protein was inhibited when preincubated for more than 5 min with a 2-fold molar excess ofα-1-antitrypsin. Chymotrypsin-like cationic protein interaction with several platelet reactions suggests a close relationship between neutrophils and platelets in the inflammatory process.
Similar content being viewed by others
References
Aledort, L.M. 1971. Platelet aggregation.In The Circulating Platelet. S.A. Johnson, editor. Academic Press, New York, 259.
Benveniste, J., P.M. Henson, andC.G. Cochrane. 1972. Leucocyte dependent histamine release from rabbit platelets. The role of IgE, basophils, and a platelet-activating factor.J. Exp. Med. 136:1356.
Born, G.V.R., andM.S. Cross. 1963. The aggregation of blood platelets.J. Physiol. (London) 168:178.
Cochrane, C.G. 1971. Mechanisms involved in the deposition of immune complexes in tissues.J. Exp. Med. 134 (3, Pt 2):75.
Cochrane, C.G., andA. Janoff. 1974. The Arthus reaction.In The Inflammatory Process, 2nd ed. Vol III. B.S. Zweifach, L. Grant, and R.T. McClusky, editors. Academic Press, New York, 85.
Davey, M.G., andE.F. Lüscher. 1967. Action of thrombin and other coagulant and proteolytic enzymes on blood platelets.Nature (London) 216:857.
Grette, K. 1962. Studies on the mechanism of thrombin catalyzed hemostatic reactions in blood platelets.Acta Physiol. Scand. Suppl. 56:195.
Henson, P.M. 1970. Mechanism of release of constituents from rabbit platelets by antigen-antibody complexes and complement. II. Interaction of platelets with neutrophils.J. Immunol. 105:490.
Hällgren, R., andP. Venge. 1976. Cationic proteins of human granulocytes. Enhancement of phagocytosis of staphylococcus protein A-IgG complexes.Inflammation 1:237.
Jenkins, C.S.P., M.A. Packham, R.L. Kinlough-Rathbone, andJ.F. Mustard. 1971. Interactions of polylysine with platelets.Blood 37:395.
Laurell, C.-B. 1965. Antigen-antibody crossed electrophoresis.Anal. Biochem. 10:358.
Mancini, G., A.O. Carbonara, andJ.F. Heremans. 1965. Immunochemical quantitation of antigen by single radial immunodiffusion.Immunochemistry 2:235.
Massini, P. 1970. Specific activity of14C-labelled serotonin from human blood platelets.Experientia 26:87.
Massini, P., andE.F. Lüscher. 1972. On the mechanism by which cell contact induces the release reaction of blood platelets: The effect of cationic polymers.Thromb. Diath. Haemorrh. 21: 121.
Miller, J.L., A.J. Katz, andM.B. Feinstein. 1975. Plasmin inhibition of thrombin-induced platelet aggregation.Thromb. Diath. Haemorrh. 33:286.
Niewiarowski, S., A.F. Senyi, andP. Gillies. 1973. Plasmin-induced platelet aggregation and platelet release reaction. Effects on hemostasis.J. Clin. Invest. 52:1647.
Odeberg, H., I. Olsson, andP. Venge. 1975. Cationic proteins of human granulocytes. IV. Esterase activity.Lab. Invest. 32:86.
Olsson, I., andP. Venge. 1974. Cationic proteins of human granulocytes. II. Separation of the cationic proteins of the granules of leukemic myeloid cells.Blood 44:235.
Pfueller, S.L., andE.F. Lüscher. 1972. The effects of aggregated immunoglobulins on human blood platelets in relation to their complement fixing abilities. I. Studies of immunoglobulins of different types.J. Immunol. 109:517.
Venge, P. Cationic proteins of human granulocytes. Isolation, characterization, and biological activities. Dissertation, Acta Universitas Upsaliensis.
Venge, P., I. Olsson, andH. Odeberg. 1975. Cationic proteins of human granulocytes. V. Interaction with plasma protease inhibitors.Scand. J. Clin. Lab. Invest. 35:737.
Wroblewski, F., andJ.S. La Due. 1955. Lactic dehydrogenase activity in blood.Proc. Soc. Exp. Biol. Med. 90:210.
Zucker, M.B. 1974. Platelets.In The Inflammatory Process, 2nd ed., Vol. I. B.S. Zweifach, L. Grant, and R.T. McCluskey, editors. Academic Press, New York, 511.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hällgren, R., Venge, P. Cationic proteins of human granulocytes Effects on human platelet aggregation and serotonin release. Inflammation 1, 359–370 (1976). https://doi.org/10.1007/BF00920337
Issue Date:
DOI: https://doi.org/10.1007/BF00920337