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Effects of Nothrombel on Thrombin-Induced Formation of Thrombocyte-Leukocyte Complexes

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The effects of Nothrombel on the thrombin-induced formation of thrombocyte-leukocyte complexes (TLC) were studied. Nothrombel was found to produce dose-dependent inhibition of TLC formation. Its activity was greater than that of reference drug aspirin. The 50% inhibition constant (EC50) of Nothrombel was 1.75 μmol/ml, compared with greater than 1.75 μmol/ml for aspirin. The mechanism of inhibition of TLC formation by Nothrombel relates to the ability of this drug to suppress the expression of P-selectin by thrombocytes and the membrane complex GPIb-IX-V on membranes and the mobilization of cytoplasmic calcium in thrombocytes, and, evidently, an inhibitory action on the P2Y12 purine receptors of thrombocytes.

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Authors and Affiliations

  1. Vertex Company, St. Petersburg, Russia

    O. S. Veselkina & M. E. Borovitov

  2. Almazov North-Western Federal Medical Research Center, St. Petersburg, Russia

    N. N. Petrishchev & L. V. Vasina

  3. Ott Research Institute of Obstetrics, Gynecology, and Reproductive Medicine, St. Petersburg, Russia

    A. V. Selyutin, S. V. Chepanov & S. A. Sel’kov

Authors
  1. O. S. Veselkina
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  2. N. N. Petrishchev
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  3. L. V. Vasina
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  4. M. E. Borovitov
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  5. A. V. Selyutin
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  6. S. V. Chepanov
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  7. S. A. Sel’kov
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Correspondence to L. V. Vasina.

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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 103, No. 3, pp. 318–326, March, 2017.

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Veselkina, O.S., Petrishchev, N.N., Vasina, L.V. et al. Effects of Nothrombel on Thrombin-Induced Formation of Thrombocyte-Leukocyte Complexes. Neurosci Behav Physi 48, 830–834 (2018). https://doi.org/10.1007/s11055-018-0636-y

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  • DOI: https://doi.org/10.1007/s11055-018-0636-y

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