Abstract
Sera and leukemic blasts of 14 patients with acute myelogenous leukemia were stored at −70°C. In eight patients in whom a remission was achieved, peripheral blood lymphocytes were cultured together with irradiated autologous leukemic cells in treated serum (serum adsorbed with protein A Sepharose) or control serum (Sepharose-treated). Lymphocyte activation was determined after 7 days in culture by [3H]thymidine incorporation. In the absence of stored leukemic blasts, significantly more [3H]thymidine incorporation occurred in six of the eight patients' lymphocytes cultured in treated serum compared to control. Enhanced activity was observed in all eight patients when irradiated leukemic blasts were cocultured with autologous lymphocytes in treated serum. In five patients, the addition of 10% or more of control serum to treated serum inhibited lymphocyte mitogenesis. Protein A immunoadsorption may allow increased stimulation of acute myelogenous leukemia remission peripheral blood lymphocytes, which is further enhanced in some patients by the presence of autologous leukemic cells. This change in lymphocyte activation may contribute to the antitumor effects of treating serum with protein A.
Similar content being viewed by others
References
Hellström I, Hellström KE, Evans CA,et al.: Serum mediated protection of neoplastic cells from inhibition by lymphocytes immune to their tumor-specific antigens. Proc Natl Acad Sci USA 62:362–368, 1969
Baldwin RW, Price MR, Rabins RA: Significance of serum factors modifying cellular immune responses to growing tumors. Br J Cancer 28:37–47, 1973
Hellström KE, Hellström I: Lymphocyte-mediated cytotoxicity and blocking serum activity to tumor antigens. Adv Immunol 18:209–277, 1974
Steele G Jr, Ankerst J, Sjögren HO: Alteration ofin vitro anti-tumor activity of tumor-bearer sera by absorption withStaphylococcus aureus, Cowan I. Int J Cancer 14:83–92, 1974
Bansal SC, Bansal BR, Thomas HL,et al.:Ex vivo removal of serum IgG in a patient with colon carcinoma: Some biochemical, immunological and histological observations. Cancer 42:1–18, 1978
Terman DS, Young JB, Shearer WT,et al.: Preliminary observations of the effects on breast adenocarcinoma of plasma perfused over immobilized protein A. N Engl J Med 305:1195–1200, 1981
Ray PK, Indiculla A, Mark R,et al.: Extracorporeal immunoadsorption of plasma from a metastatic colon carcinoma patient by protein A-containing nonviableStaphylococcal aureus: Clinical, biochemical, serologic, and histologic evaluation of the patient's response. Cancer 49:1800–1809, 1982
Bensinger WI, Kinet JP, Hennen G,et al.: Plasma perfused over immobilized protein A for breast cancer (letter). N Engl J Med 306:935–936, 1982
Miller WJ, Branda RF, Hurd DD,et al.: Protein A adsorption of acute myelogenous leukemia serum inducesin vitro blast lysis. Blood 59:1344–1347, 1982
Jones FR, Yoshida LH, Ladiges WC, Kenny MA: Treatment of feline leukemia and reversal of FeLV byex vivo removal of IgG: A preliminary report. Cancer 46:675–684, 1980
Boyum A: Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest (Suppl 97) 21:77–89, 1968
Oppenheim JJ, Schecter B: Lymphocyte transformation.In Manual of Clinical Immunology, 2nd ed, NR Rose, H Friedman (eds). Washington DC, American Society for Microbiology, 1980, pp 236–237
Axen R, Porath J, Ernback S: Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature 214:1302–1304, 1967
Kyle RA: Classification and diagnosis of monoclonal gammopathies.In Manual of Clinical Immunology, 2nd ed, NR Rose, H Friedman (eds). Washington DC, American Society for Microbiology, 1980, pp 137–138
Buffone GJ: Immunonephelometric and turbidimetric measurement of specific plasma proteins.In Manual of Clinical Immunology, 2nd ed, NR Rose, H Friedman (eds). Washington DC, American Society for Microbiology, 1980, pp 27–28
Gutterman JU, Rossen RD, Butler WT,et al.: Immunoglobulin on tumor cells and tumor-induced lymphocyte blastogenesis in human acute leukemia. N Engl J Med 288:169–173, 1973
Romagnani S, Amadori A, Giudizi MG,et al.: Different mitogenic activity of soluble and insoluble staphylococcal protein A (SPA). Immunology 35:471–478, 1978
Korec S, Phillips TM, Djeu JY,et al.: Complex immunological changes in cancer patients induced by perfusing of plasma over filters containing staphylococcal protein A (SPA). Proc Am Soc Clin Oncol 2:51, 1983 (abstr)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Spitzer, T.R., Lazarus, H.M. Effects of staphylococcal protein A-treated human leukemic serum on autologous leukemic blast growth and mitogenesis of lymphocytes. J Clin Immunol 4, 455–460 (1984). https://doi.org/10.1007/BF00916575
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00916575