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A rapid quantitative assay for activated neutrophils

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Abstract

We present here a rapid, sensitive, and convenient assay for activated human PMNs based on detecting the decreased optical density (OD) of aggregated cell suspensions. This quantitative assay uses an ELIZA machine to measure OD changes, with time, of activated cells (5 × 105 cells/well) in microtiter plates. The assay is sensitive, detecting aggregation induced by as little as 0.0001 μg/ml of LPS, or lymphokines in Con-A-activated supernatant diluted 1/500. The assay permits analysis of 400 separate PMN suspensions on the same day starting with less than 80 ml of blood.

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Authors and Affiliations

  1. School of Dentistry and Departments of Microbiology and Biochemistry, Oregon Health Sciences University, 97201, Portland, Oregon

    Wesley W. Bullock, Howard R. Creamer & Walter L. Gabler

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  1. Wesley W. Bullock
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  2. Howard R. Creamer
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  3. Walter L. Gabler
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This research was funded by a grant from the Kroc Foundation.

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Bullock, W.W., Creamer, H.R. & Gabler, W.L. A rapid quantitative assay for activated neutrophils. Inflammation 6, 305–318 (1982). https://doi.org/10.1007/BF00916411

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