Abstract
Neutrophils are crucial cells for host defence. In particular, their phagocytic function is a major mechanism through which the immune system eliminates extracellular pathogenic microorganisms. The production of reactive oxygen species (ROS) by neutrophils, referred to as the ‘oxidative burst’, is a critical step in the destruction of these phagocytosed bacteria. Flow cytometry is an ideal technology to use for the measurement of these functions. In this paper I describe methods for doing this that can be applied to the study of bacteria-neutrophil interactions and used in the clinical diagnosis of neutrophil defects and as a measure of an individual’s immune system status.
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Hutchinson, P. (2022). Flow Cytometry for Measuring Neutrophil Function. In: Sobti, R., Sobti, A. (eds) Biomedical Translational Research. Springer, Singapore. https://doi.org/10.1007/978-981-16-4345-3_18
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DOI: https://doi.org/10.1007/978-981-16-4345-3_18
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