Skip to main content
SpringerLink
Log in

Effect of immunoglobulins on mineral dust-induced production of reactive oxygen metabolites by human macrophages

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Mineral dust-induced production of reactive oxygen metabolites (ROMs) by human monocyte-derived macrophages was investigated using lucigenin-dependent chemiluminescence. Chrysotile asbestos alone caused only weak ROM production by macrophages, but the addition of polyclonal immunoglobulin enhanced the reaction strongly. The phenomenon was seen with 1-, 4-, and 7-day-old cell cultures. Polyclonal immunoglobulin also slightly enhanced the ROM responses induced by amosite, crocidolite, and quartz dust. The enhancing effect could be achieved with several monoclonal immunoglobulins (isolated from the sera of myeloma patients), but IgA and IgG had the strongest effects. We suggest that immunoglobulins may interact with mineral dusts in a “nonimmunological,” antigen-independent way and that the so-formed dust-immunoglobulin complexes may amplify the production of ROMs by inflammatory cells. This may explain a number of in vivo phenomena in which immune responses (for instance hypergammaglobulinemia and the presence of autoantibodies) have been shown to relate to the progression of mineral dust-induced pulmonary disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Craighead, J. E., andB. T. Mossman. 1982. The pathogenesis of asbestos-associated diseases.N. Engl. J. Med. 306:1446–1455.

    Google Scholar 

  2. Davis, G. S. 1986. Pathogenesis of silicosis: Current concepts and hypotheses.Lung 164:139–154.

    Google Scholar 

  3. Allison, A. C., J. S. Harington, andM. Birbeck. 1966. An examination of the cytotoxic effects of silica on macrophages.J. Exp. Med. 124:141–153.

    Google Scholar 

  4. Permis, B., andE. C. Vigliani. 1982. The role of macrophages and immunocytes in the pathogenesis of pulmonary diseases due to mineral dusts.Am. J. Ind. Med. 3:133–137.

    Google Scholar 

  5. Doll, N. J., R. P. Stankus, andH. W. Barkman. 1983. Immunopathogenesis of asbestosis, silicosis, and coal workers's pneumoconiosis.Clin. Chest Med. 4:3–14.

    Google Scholar 

  6. Babior, B. M. 1978. Oxygen-dependent microbial killing by phagocytes.N. Engl. J. Med. 298:659–668.

    Google Scholar 

  7. Halliwell, B., andM. Grootveld. 1987. The measurement of free radical reactions in humans. Some thoughts for future experimentation.FEBS Lett. 213:9–14.

    Google Scholar 

  8. Allen, R. C. 1981. Lucigenin chemiluminescence: a new approach to the study of polymorphonuclear leukocyte redox activity.In Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications. M. DeLuca and W. D. McElroy, editors. Academic Press, New York. 63–73.

    Google Scholar 

  9. Williams, A. J., andP. J. Cole. 1981. Investigation of alveolar macrophage function using lucigenin-dependent chemiluminescence.Thorax 36:866–869.

    Google Scholar 

  10. Aasen, T. B., B. Bolann, J. Glette, R. J. Ulvik, andA. Schreiner. 1987. Lucigenin-dependent chemiluminescence in mononuclear phagocytes. Role of superoxide anion.Scand. J. Clin. Lab. Invest. 47:673–679.

    Google Scholar 

  11. Gyllenhammar, H. 1987. Lucigenin chemiluminescence in the assessment of neutrophil superoxide production.J. Immunol. Methods 97:209–213.

    Google Scholar 

  12. Doll, N. J., R. P. Stankus, S. Goldbach, andJ. E. Salvaggio. 1982. In vitro effect of asbestos fibers on polymorphonuclear leukocyte function.Int. Arch. Allergy Appl. Immunol. 68:17–21.

    Google Scholar 

  13. Rola-Pleszczynski, M., D. Rivest, andM. Berardi. 1984. Asbestos-induced chemiluminescence response of human polymorphonuclear leukocytes.Environ. Res. 33:1–6.

    Google Scholar 

  14. Hedenborg, M., andM. Klockars. 1987. Production of reactive oxygen metabolites induced by asbestos fibres in human polymorphonuclear leukocytes.J. Clin. Pathol. 40:1189–1193.

    Google Scholar 

  15. Hedenborg, M., andM. Klockars. 1989. Quartz-dust-induced production of reactive oxygen metabolites by human granulocytes.Lung 167:23–32.

    Google Scholar 

  16. Hatch, G. E., D. E. Gardner, andD. B. Menzel 1980. Stimulation of oxidant production in alveolar macrophages by pollutant and latex particles.Environ. Res. 23:121–136.

    Google Scholar 

  17. Vilim, V., J. Wilhelm, P. Brzak, andJ. Hurych. 1984. The chemiluminescence of rabbit alveolar macrophages induced by quartz dust particles.Immunol. Lett. 8:69–73.

    Google Scholar 

  18. Donaldson, K., andR. T. Cullen. 1984. Chemiluminescence of asbestos-activated macrophages.Er. J. Exp. Pathol. 65:81–90.

    Google Scholar 

  19. Klockars, M., M. Hedenborg, J. Koistinen, andT. Isobe. 1989. IgG and IgA enhance the chrysotile induced production of reactive oxygen metabolites by human PMN.Clin. Exp. Immunol. 78:372–377.

    Google Scholar 

  20. Bøyum, A. 1968. Isolation of mononuclear cells and granulocytes from human blood.Scand. J. Clin. Lab. Invest. 21(Suppl. 97):77–89.

    Google Scholar 

  21. Nyberg, P., andM. Klockars. 1990. Measurement of reactive oxygen metabolites produced by human monocyte-derived macrophages exposed to mineral dusts.Int. J. Exp. path. 71:537–544.

    Google Scholar 

  22. Rendall, R. E. 1970. The data sheets on the chemical and physical properties of the UICC standard reference samples.In Pneumoconiosis: Proceedings of the International Conference, Johannesburg, Capetown, Union of South Africa. H. A. Shapiro, editor. Oxford University Press, Oxford, U.K. 23–27.

    Google Scholar 

  23. Timbrell, V. 1970. Characteristics of the International Union against cancer standard reference samples.In Pneumoconiosis: Proceedings of the International Conference, Johannesburg, Capetown, Union of South Africa. H. A. Shapiro, editor. Oxford University Press, Oxford, U.K. 28–36.

    Google Scholar 

  24. Tossavainen, A. 1979. Determination of quartz on membrane filters by x-ray diffraction.Scand. J. Work. Environ. Health 5:379–385.

    Google Scholar 

  25. Isobe, T., andE. F. Osserman 1974. Plasma cell dyscrasia associated with the production on incomplete (?deleted) IgG molecules, gamma heavy chains, and free lambda chains containing carbohydrate: Description of the first case.Blood 43:505–515.

    Google Scholar 

  26. Haimovitz, A., Z. Fuks, M. Rubenstein, andA. J. Treves. 1983. The instability of membrane markers expressed by human monocytes and macrophages in culture.Immunobiology 165:432–444.

    Google Scholar 

  27. Abramson, S., S. Hoffstein, andG. Weissmann. 1982. Superoxide anion generation by human neutrophils exposed to monosodium urate.Arthritis Rheum. 25:174–180.

    Google Scholar 

  28. Kozin, F., andD. McCarty. 1980. Molecular orientation of immunoglobulin G adsorbed to microcrystalline monosodium urate monohydrate.J. Lab. Clin. Med. 95:49–59.

    Google Scholar 

  29. Nagase, M., D. G. Baker, andH. R. Schumacher. 1989. Immunoglobulin G coating on crystals and ceramics enhance polymorphonuclear cell superoxide production: Correlation with immunoglobulin G adsorbed.J. Rheumatol. 16:971–976.

    Google Scholar 

  30. Hasselbacher, P. 1979. Binding of immunoglobulin and activation of complement by asbestos fibers.J. Allergy din. Immunol. 64:294–298.

    Google Scholar 

  31. Turner-Warwick, M., andW. R. Parkes 1970. Circulating rheumatoid and antinuclear factors in asbestos workers.Br. Med. J. 3:492–495.

    Google Scholar 

  32. Lange, A., R. Smolik, W. Zatonski, andJ. Szymanska. 1974. Autoantibodies and serum immunoglobulin levels in asbestos workers.Int. Arch. Arbeitsmed. 32:313–325.

    Google Scholar 

  33. Zone, J. J., andW. N. Rom. 1985. Circulating immune complexes in asbestos workers.Environ. Res. 37:383–389.

    Google Scholar 

  34. Begin, R., A. Cantin, Y. Berthiaume, R. Boileau, G. Bisson, G. Lamoureux, M. Rola-Pleszczynski, G. Drapeau, S. Massé, M. Boctor, J. Breault, S. Peloquin, andD. Dalle. 1985. Clinical features to stage alveolitis in asbestos workers.Am. J. Ind. Med. 8:521–536.

    Google Scholar 

  35. Becklake, M. R. 1982. Exposure to asbestos and human disease.N. Engl. J. Med. 306:1480–1482.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Occupational Health, Topeliuksenkatu 41 a A, SF-00250, Helsinki, Finland

    Peter Nyberg & Matti Klockars

Authors
  1. Peter Nyberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Matti Klockars
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nyberg, P., Klockars, M. Effect of immunoglobulins on mineral dust-induced production of reactive oxygen metabolites by human macrophages. Inflammation 14, 621–629 (1990). https://doi.org/10.1007/BF00916366

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00916366

Keywords

Search

Navigation