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Effect of gold compounds on nadph oxidase system of human neutrophils

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Abstract

The inhibitory effects of gold compounds on the NADPH oxidase system of human polymorphonuclear leukocytes (PMNs) has been investigated. Auranofin (0.5-4.0 μg An/ml) suppressed the rate of Superoxide anion generation as well as the total yield in cells stimulated with phorbol myristate acetate and f-Met-Leu-Phe. This implies that drug action may be occurring at the level of protein kinase C or steps subsequent to this in the signal transduction sequence. Sodium aurothiomalate (1–100 μg Au/ml) lacked such activity. Neither gold compound altered the ability of the granule-rich fraction of PMNs to produce oxy radicals whether this fraction was obtained from drug-treated cells or was treated after its isolation. Therefore, in order for auranofin to exhibit its inhibitory effects on the NADPH oxidase system, an intact cell membrane is necessary.

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Authors and Affiliations

  1. The Division of Rheumatology, University of Alberta, 9-112 Clinical Sciences Building, Edmonton, Alberta, Canada

    Janice E. Parente, Kenneth Wong & Paul Davis

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  1. Janice E. Parente
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  2. Kenneth Wong
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  3. Paul Davis
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Parente, J.E., Wong, K. & Davis, P. Effect of gold compounds on nadph oxidase system of human neutrophils. Inflammation 10, 303–310 (1986). https://doi.org/10.1007/BF00916125

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