Abstract
Purified polymorphonuclear (PMN) cells were obtained from human blood leukocytes by centrifugation on colloidal silica gradients. PMNs could be primed for PMA-triggered oxidative burst by muramyl peptide molecules (MDP) and two of its adjuvant active nonpyrogenic derivatives. The priming effect of MDP could be demonstrated after a 1-h incubation period, whereas monocytes needed an 18-h incubation to produce an enhanced response in the NBT reduction test. Only the monocyte-enriched population was able to produce IL-1 activity after muramyl peptide stimulation. Under such conditions, PMNs neither produced nor secreted IL-1-like activity, and no IL-1 inhibitor was present in the supernatant fluids. In conclusion, muramyl peptides were able to prime PMNs for oxidative burst but not to stimulate IL-1-like factor production.
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Osada, Y., T. Otani, M. Sato, T. Une, K. Matsumoto, andH. Ogawa. 1982. Polymorphonuclear leukocyte activation by a synthetic muramyl dipeptide analog.Infect. Immun. 38:848–854.
Chedid, L., M. Parant, F. Parant, P. Lefrancier, J. Choay andE. Lederer. 1977. Enhancement of nonspecific immunity to Klebsiella pneumoniae infection by a synthetic immunoadjuvant (N-acetyl muramyl-l-alanyl-d-isoglutamine) and several analogs.Proc. Natl. Acad. Sci. U.S.A. 74:2089–2093.
Morikawa, K., R. Takeda, M. Yamazaki, andD. Mizuno. 1985. Induction of tumoricidal activity of polymorphonuclear leukocytes by a linearβ-1,3-d-glucan and other immunomodulators in murine cells.Cancer Res. 45:1469–1501.
Kaku, M., K. Yagawa, S. Nagao, andA. Tanaka. 1983. Enhanced superoxide anion release from phagocytes by muramyl dipeptide or lipopolysaccharide.Infect. Immun. 39: 559–564.
Giroud, J. P., M. Roch-Arveiller, O. Muntaner, andD. Bradshaw. 1980. Action comparée du lévamisole, du muramyl dipeptide et de la tuftsine sur le chimiotactisme des polynucléaires de rat.Nouv. Rev. Fr. Hematol. 22:69–76.
Ogawa, T., H. Takada, S. Kotani, S. Kusumoto, T. Shiba, S. Kawata, K. Yokogawa, andA. Inoue. 1981. Basophile leukocyte stimulation by bacterial cell walls and related compounds.In Immunomodulation by Microbial Products and Related Synthetic Compounds. Y. Yamamura, S. Kotani, I. Azuma, A. Koda, and T. Shiba, editors. Excerpta Medica, Amsterdam. 221–224.
Ishihara, Y., H. Takada, S. Kotani, S. Kusumoto, T. Shiba, S. Kawata, andK. Kokogawa. 1981. Stimulation of polymorphonuclear leukocytes by bacterial cell wall components and related synthetic compounds.In Immunomodulation by Microbial Products and Related Synthetic Compounds. Y. Yamamura, S. Kotani, I. Azuma, A. Koda, and T. Shiba, editors. Excerpta Medica, Amsterdam. 217–220.
Beeson, P. 1948. Temperature elevating effect of a substance obtained from polymorphonuclear leukocytes.J. Clin. Invest. 27:524 (abstract).
Dinarello, C., N. Goldin, andS. Wolff. 1974. Demonstration and characterization of two distinct human leukocytic pyrogens.J. Exp. Med. 139:1369–1381.
Yoshinaga, M., S., Nakamura, andH. Hayashi. 1975. Interaction between lymphocytes and inflammatory exudate cells. I. Enhancement of thymocyte response to PHA by product(s) of polymorphonuclear leukocytes and macrophages.J. Immunol. 115:533–538.
Yoshinaga, M., F. Goto, K. Goto, S. Ohkawara, S. Nakamura, M. Kitamura, andH. Takahashi. 1985. Biosynthesis and purification of IL-1 like factor from polymorphonuclear leukocytes of rabbits with tumor-induced granulocytosis.In The Physiologic, Metabolic, and Immunologic Actions of Interleukin-1. M. J. Klüger, J. J. Oppenheim, and M. Powanda, editors. Alan R. Liss, New York. 385–398.
Goto, F., S. Nakamura, K. Goto, andM. Yoshinaga, 1984. Production of a lymphocyte proliferation potentiating factor by purified polymorphonuclear leucocytes from mice and rabbits.Immunology 53:638–691.
Goto, K., S. Nakamura, F. Goto, andM. Yoshinaga. 1984. Generation of an interleukin 1-like lymphocyte-stimulating factor at inflammatory sites: Correlation with the infiltration of polymorphonuclear leucocytes.Br. J. Exp. Pathol. 65:521–532.
Windle, B. E., P. Murphy, andS. Cooperman. 1983. Rabbit polymorphonuclear leukocytes do not secrete endogenous pyrogens or interleukin 1 when stimulated by endotoxin, polyinosine: polycytosine, or muramyl dipeptide.Infect. Immun. 39:1142–1146.
Damais, C., G. Riveau, M. Parant, J. Gerota, andL. Chedid. 1982. Production of lymphocyte activating factor in the absence of endogenous pyrogen by rabbit or human leukocytes stimulated by a muramyl dipeptide derivative.Int. J. Immunopharmacol. 4:451–462.
Lefrancier, P., M. Derrien, X. Jamet, J. Choay, E. Lederer, F. Audibert, M. Parant, F. Parant, andL. Chedid. 1982. Apyrogenic adjuvant-activeN-acetyl-muramyl dipeptides.J. Med. Chem. 25:87–90.
Feige, U., B. Overwen, andC. Sorg. 1982. Purification of human blood monocytes by hypotonic density gradient centrifugation in Percoll.J. Immunol. Methods 54:309–315.
Bertoglio, J., N. Boisson, M. Bonnet, L. Chatenoud, S. Chouaib, G. Degiovanni, D. Fradelizi, A. Godard, P. Goube de la Foret, A. Harel-Bellan, R. Jacubovich, M. Lacaze, T. Lahaye, M. Mousalayi, M. Pla, A. Rey, E. Rotschild, C. Schmitt, B. Serrou, J. Soulillou, F. Tribel, andA. Ythier. 1982. First French workshop on standardization of human IL-2: Joint report.Lymphokine Res. 1:121–127.
Pick, E., J. Charon, andD. Mizel. 1981. A rapid densitometric micro-assay for nitroblue tetrazolium reduction and application of the microassay to macrophages.J. Res. 30:581–593.
Pabst, M., H. Hedesgaard, andR. Johnston. 1982. Cultured human monocytes require exposure to bacterial products to maintain an optimal oxygen radical response.J. Immunol. 128:123–128.
Chedid, L., M. Parant, F. Audibert, G. Riveau, F. Parant, E. Lederer, J. Choay, andP. Lefrancier. 1982. Biological activity of a new synthetic muramyl peptide adjuvant devoid of pyrogenicity.Infect. Immun. 35:417–424.
Tiku, K., J. Skosey, andM. Tiku. 1985. Neutrophil production of an interleukin-1 inhibitor.J. Leuk. Biol. 37:747 (abstract).
Tiku, K., M. Tiku, andJ. Skosey. 1985. Description of a neutrokine: A neutrophil derived thymocyte activating factor with interleukin-1-like activity.J. Leuk. Biol. 37:748 (abstract).
Petrone, W., D. English, K. Wong, andJ. McCord. 1980. Free radicals and inflammation: Superoxide-dependent activation of a neutrophil chemotactic factor in plasma.Proc. Natl. Acad. Sci. U.S.A. 77:1159–1163.
Atkins, E., P. Bodel, andL. Francis. 1967. Release of an endogenous pyrogen in vitro from rabbit mononuclear cells.J. Exp. Med. 126:357–384.
Hahn, H., D. Char, W. Postel, andW. B. Wood. 1967, The production of endogenous pyrogen by peritoneal macrophages.J. Exp. Med. 126:385–394.
Kampschmidt, R. 1978. Leukocytic endogenous mediator.J. Reticuloendothel. Soc. 23:287–297.
Hanson, D., P. Murphy, andB. Windle. 1980. Failure of rabbit neutrophils to secrete endogenous pyrogen when stimulated with staphylococci.J. Exp. Med. 151:1360–1371.
Kampschmidt, R. 1981. Leukocytic endogenous mediator/endogenous pyrogen.In Infection. The Physiologic and Metabolic Responses of the Host. M. C. Powanda and P. G. Canonico, Editors. Elsevier/North Holland Press, Amsterdam. 56–74.
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This work was supported in part by CNRS ATP-955335.
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Jupin, C., Parant, M., Chedid, L. et al. Enhanced oxidative burst without interleukin 1 production by normal human polymorphonuclear leukocytes primed with muramyl dipeptides. Inflammation 11, 153–161 (1987). https://doi.org/10.1007/BF00916017
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DOI: https://doi.org/10.1007/BF00916017