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Decreased extracellular release of granule enzymes from in vitro-stimulated polymorphonuclear leukocytes in guttate psoriasis

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Abstract

In vitro degranulation of polymorphonuclear leukocytes, which were stimulated either with synthetic chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine, FMLP) or with C3b-opsonized zymosan as a promoter of phagocytosis, was studied in 66 patients with psoriasis, 18 lesion-free psoriatics, 18 healthy subjects, and 14 other dermatological disorder controls. Stimulated release of lysozyme (from specific granules and azurophil granules) and beta-glucuronidase (from azurophil granules) in the presence of both FMLP and serum-activated zymosan was markedly reduced in patients with actively spreading guttate psoriatic lesions, in whom relapse of lesions lasted for less than 1 month and papules involved about 13–25% of skin surface. In contrast, stimulated degranulation was within normal range in active plaque psoriasis, stationary plaque psoriasis, symptomless psoriatics, and patients with disseminated eczema. Spontaneous release of lysozyme and beta-glucuronidase (background) was found to be not different in all groups studied; however, patients with active guttate psoriasis had significantly lower total lysozyme activity than those with active and stationary plaque psoriasis as well as psoriatics in the remission. These data are in favor of in vivo activation of neutrophils in active guttate psoriasis by some factors related to the early relapse of the lesions. This results in a possible combination of the following phenomena: (1) in vivo partial degranulation of neutrophils; (2) induction of “unresponsiveness state” of these cells to subsequent in vitro stimulation; and/or (3) migration of highly responsive neutrophils to skin lesions, which leaves in the circulation the subpopulation less reactive to chemotactic and phagocytic stimuli.

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References

  1. Sedgwick, J. B., P. R. Bergstresser, andE. R. Hurd. 1980. Increased granulocyte adherence in psoriasis and psoriatic arthritis.J. Invest. Dermatol. 74: 81–84.

    Google Scholar 

  2. Glinski, W., M. Haftek, S. Obalek, andH. Sochor. 1978. Immunological abnormalities in psoriasis: The inhibition of leukocyte migration by stratum corneum antigens.Dermatologica 156:231–237.

    Google Scholar 

  3. Langner, A., T. P. Ckorzelski, M. Fraczykowska, S. Jablonska, andJ. Szymanczyk. 1983. Is chemotactic activity of polymorphonuclear leukocytes increased in psoriasis?Arch. Dermatol. Res. 275:226–228.

    Google Scholar 

  4. Michaelsson, G. 1980. Increased chemotactic activity of neutrophil leukocytes in psoriasis.Br. J. Dermatol. 103:351–356.

    Google Scholar 

  5. Wahba, A., H. A. Cohen, M. Bar-Eli andR. Gallily. 1978. Enhanced chemotactic and phagocytic activities of leukocytes in psoriasis vulgaris.J. Invest. Dermatol. 71:186–188.

    Google Scholar 

  6. Kawohl, G., B. Szperalski, J. M. Schroder, andE. Christophers. 1980. Polymorphonuclear leukocyte chemotaxis in psoriasis: Enhancement by self-activated serum.Br. J. Dermatol. 103:527–533.

    Google Scholar 

  7. Herlin, T., N. Borregaard, andK. Kragballe. 1982. On the mechanisms of enhanced monocyte and neutrophil cytotoxicity in severe psoriasis.J. Invest. Dermatol. 79:104–108.

    Google Scholar 

  8. Lundin, A., L. Hahansson, R. Hallgren, G. Michaelsson,andP. Venge. 1983. Studies on the phagocytic activity of the granulocytes in psoriasis and palmoplantar pustulosis.Br. J. Dermatol. 109:539–547.

    Google Scholar 

  9. Csato, M., Z. Kabok, andA. Dobozy. 1984. Increased IgG Fc and C3 rosette-forming capacity of granulocytes from patients with psoriasis.Allergy 39:69–72.

    Google Scholar 

  10. Preissner, W. C., J. M. Schroder, andE. Christophers. 1983. Altered polymorphonuclear leukocyte responses in psoriasis: Chemotaxis and degranulation.Br. J. Dermatol. 109:1–9.

    Google Scholar 

  11. Sedgwick, J. B., P. R. Bergstresser, andE. R. Hurd. 1981. Increased superoxide generation by normal granulocytes incubated in sera from patients with psoriasis.J. Invest. Dermatol. 76:158–163.

    Google Scholar 

  12. Krueger, G. G., H. R. Hill, andW. W. Jederberg. 1978. Inflammatory and immune cell function in psoriasis—a subtle disorder. I. In vivo and in vitro survey.J. Invest. Dermatol. 71:189–194.

    Google Scholar 

  13. Breathnach, S. M., P. Carrington, andM. M. Black. 1981. Neutrophil leukocyte migration in psoriasis vulgaris.J. Invest. Dermatol. 76:271–274.

    Google Scholar 

  14. Fraki, J. E., L. Jakoi, A. O. Davies, R. J. Lefkowitz, R. Snyderman, andG. S. Lazarus. 1983. Polymorphonuclear leukocyte function in psoriasis: Chemotaxis, chemokinesis, betaadrenergic receptors, and proteolytic enzymes of polymorphonuclear leukocytes in the peripheral blood from psoriatic patients.J. Invest. Dermatol. 81:254–257.

    Google Scholar 

  15. Wahba, A., H. A. Cohen, M. Bar-Eli, andM. Gallily. 1979. Neutrophil chemotaxis in psoriasis.Acta Dermatovenereol. 59:441–445.

    Google Scholar 

  16. Tigalonowa, M., W. Glinski, andS. Jablonska. 1983. In vivo mobilization of polymorphonuclear leukocytes in psoriasis: Relationship to clinical parameters and serum inhibitory factors.J. Invest. Dermatol 81:6–9, 1983.

    Google Scholar 

  17. Brittinger, G., R. Hirschhorn, S. D. Douglas, andG. Weissmann. 1968. Studies on lysosomes. XI. Characterization of a hydrolase rich fraction from human leukocytes.J. Cell Biol. 37:394–411.

    Google Scholar 

  18. Wright, D. G., andJ. I. Gallin. 1979. Secretory responses of human neutrophils: Exocytosis of specific (secondary) granules by human neutrophils during adherence in vitro and during exudation in vivo.J. Immunol. 123:285–294.

    Google Scholar 

  19. Gallin, J. I., D. G. Wright, andE. Schiffmann. 1978. Role of secretory events in modulating human neutrophil chemotaxis.J. Clin. Invest. 62:1364–1374.

    Google Scholar 

  20. Bentwood, B. J., andP. M. Henson. 1980. The sequential release of granule constituents from human neutrophils.J. Immunol. 124:855–862.

    Google Scholar 

  21. Fehr, J., andC. Dahinden. 1979. Modulating influence of chemotactic factor-induced cell adhesiveness of granulocyte function.J. Clin. Invest. 64:8–16.

    Google Scholar 

  22. Snyderman, R., andE. J. Goetzl. 1981. Molecular and cellular mechanisms of leukocyte chemotaxis.Science 213:830–837.

    Google Scholar 

  23. Chenoweth, D. E., andT. E. Hugli. 1978. The C5a receptor of human polymorphonuclear leukocytes. I. Demonstration of specific C5a receptor on intact cells.Proc. Natl. Acad. Sci. U.S.A. 75:3943–3947.

    Google Scholar 

  24. Goetzl, E. J., andKum Yoke Hoe. 1979. Chemotactic factor receptors of human PMN leukocytes. I. Effects on migration of labeling plasma membrane determinants with impermeant covalent reagents and inhibition of labelling by chemotactic factors.Immunology 37:407–411.

    Google Scholar 

  25. Harvath, L., andE. J. Leonard. 1982. Two neutrophil populations in human blood with different chemotactic activities: Separation and chemoattractant binding.Infect. Immun. 36:443–449.

    Google Scholar 

  26. Klempner, M. S., andJ. I. Gallin. 1978. Separation and functional characterization of human neutrophil subpopulations.Blood 51:659–665.

    Google Scholar 

  27. Broxmeyer, H. E., P. Ralph, J. Bognacki, P. W. Kincade, andM. Desousa. 1980. A subpopulation of human polymorphonuclear neutrophils contains an active form of lactoferrin capable of binding to human monocytes and inhibiting production of granulocyte-macrophage colony stimulatory activities.J. Immunol. 125:903–909.

    Google Scholar 

  28. Seligmann, B. E., T. M. Chused, andJ. I. Gallin. 1981. Human neutrophil heterogeneity identified using flow microfluorometry to monitor membrane potential.J. Clin. Invest. 68:1125–1130.

    Google Scholar 

  29. Seligmann, B. E., D. A. Melnick, H. L. Malech, andJ. I. Gallin. 1983. Identification of two subpopulations of neutrophils using the antineutrophil antibody 3ID8 and correlation with functional responsiveness.J. Cell Biol. 97:1580 (abstract).

    Google Scholar 

  30. Dahl, M., W. E. Lindroos, andR. D. Nelson. 1978. Chemotactic and chemokinetic factors in psoriatic scale extracts.J. Invest. Dermatol. 71:402–406.

    Google Scholar 

  31. Hammarstrom, S., M. Hamberg, B. Samuelsson, E. A. Duell, M. Stawiski, andJ. J. Voorhees. 1975. Increased concentration of nonestrified arachidonic acid 12l-hydroxy 5,8,10,14-eicosatetranoic acid, prostaglandin E2 and prostaglandin F2 alpha in epidermis of psoriasis.Proc. Natl. Acad. Sci. U.S.A. 72:5130–5134.

    Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, Academy of Medicine, 02008, Warsaw, Poland

    Wieslaw Glinski, Maya Tigalonowa, Stefania Jablonska & Ewa Janczura

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  1. Wieslaw Glinski
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  2. Maya Tigalonowa
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  3. Stefania Jablonska
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  4. Ewa Janczura
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Glinski, W., Tigalonowa, M., Jablonska, S. et al. Decreased extracellular release of granule enzymes from in vitro-stimulated polymorphonuclear leukocytes in guttate psoriasis. Inflammation 10, 99–108 (1986). https://doi.org/10.1007/BF00915992

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