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Serum levels of RHP and of unbound C1q in rheumatoid arthritis and systemic lupus erythematosus

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Abstract

RHP is a recently described serum protein which inhibits a number of physiologic functions of Clq unbound to Clr2×Cls2. In this report we show that sera from patients with rheumatoid arthritis contained elevated levels of RHP and of unbound Clq. Sera from patients with systemic lupus erythematosus contained normal levels of RHP and were characterized by deficits of Clq required to form Cl from existing levels of Clr and Cls.

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References

  1. Rosano, C. L., N. Parhami, andC. Hurwitz. 1984, Serum levels of Cl sub-units in rheumatoid arthritis.J. Lab. Clin. Med. 103:313–321.

    Google Scholar 

  2. Rosano, C. L., N. Parhami, andC. Hurwitz. 1985. Isolation and purification of a Clq-anti Clq precipitin ring enhancing protein from sera of patients with rheumatoid arthritis.Biochem. Biophys. Res. Commun. 128:1288–1294.

    Google Scholar 

  3. Hurwitz, C., C. L. Rosano, K. E. Hechemy, andP. S. Satoh. 1987. Biochemical and immunological properties of RHP, a serum protein associated with rheumatoid arthritis.Fed. Proc. 46:612.

    Google Scholar 

  4. Rosano, C. L., J. A.Fox, K. E.Hechemy, N.Parhami, P. S.Satoh, and C.Hurwitz. Chemical and immunochemical studies of RHP, a serum protein elevated in rheumatoid arthritis.J. Immunol. (submitted).

  5. Rosano, C. L., N. Parhami, andC. Hurwitz. 1979. The clinical significance of protein-bound hydroxyproline fractions in sera of patients with adult rheumatoid arthritis.J. Rheum. 6:656–663.

    Google Scholar 

  6. Rosano, C. L., N. Parhami, andC. Hurwitz. 1979. Determination of Clq in human serum.J. Lab. Clin. Med. 94:593–599.

    Google Scholar 

  7. Ochi, T., K. Yonemasu, andK. Ono. 1980. Immunochemical quantification of complement components of Clq and C3 in sera and synovial fluids of patients with bone and joint diseases.Ann. Rheum. Dis. 39:235–240.

    Google Scholar 

  8. Ochi, T., K. Yonemasu, R. Iwase, T. Sasaki, I. Tsuyama, andK. Ono. 1984. Serum Clq levels as a prognostic guide to articular erosions in patients with rheumatoid arthritis.Arthritis Rheum. 27:883–887.

    Google Scholar 

  9. Rosano, C. L., C. B.Braun, K. E.Hechemy, N.Parhami, P. S.Satoh, and C.Hurwitz, 1987. Inhibition of Clq functions by RHP, a protein elevated in sera from patients with rheumatoid arthritis.Complement (in press).

  10. Reid, K. B. M., D. M. Lowe, andR. R. Porter. 1972. Isolation and characterization of Clq, a subcomponent of the first component of complement, from human and rabbit sera.Biochem. J. 130:749–763.

    Google Scholar 

  11. Prockop, D. J., andS. Udenfriend. 1960. A specific method for the analysis of hydroxy-proline in tissues and urine.Anal. Biochem. 1:228–239.

    Google Scholar 

  12. Hoekzema, R., A. J. Hannema, T. J. G. Swaak, J. Paardekooper, andC. E. Hack. 1985. Low molecular weight Clq in systemic lupus erythematosus.J. Immunol. 135:265–271.

    Google Scholar 

  13. Ziccardi, R. J., andJ. Tschopp. 1982. The dissociation properties of native Cl.Biochem. Biophys. Res. Commun. 107:618–623.

    Google Scholar 

  14. Scheffe, H. 1959. The Analysis of Variance. New York, John Wiley & Sons.

    Google Scholar 

  15. Cochran, W.G. 1964. Approximate significance levels of the Behrens-Fisher test.Biometrics 20:191–195.

    Google Scholar 

  16. Bartlett, M. S. 1937. Properties of sufficiency and statistical tests.Proc. R. Soc. A 160:268–282.

    Google Scholar 

  17. Dickler, H. B., andH. G. Kunkel. 1972. Interaction of aggregated gamma-globulin with B lymphocytes.J. Exp. Med. 136:191–196.

    Google Scholar 

  18. Gabay, Y., H. Perlman, P. Perlman, andA. T. Sobel. 1979. A rosette assay for the determination of Clq receptor-bearing cells.Eur. J. Immunol. 9:797–801.

    Google Scholar 

  19. Tenner, A. J., andN. R. Cooper. 1980. Analysis of receptor mediated Clq binding to human peripheral blood mononuclear cells.J. Immunol. 125:1658–1664.

    Google Scholar 

  20. Tenner, A. J., andN. R. Cooper. 1981. Identification of types of cells in human peripheral blood that bind Clq.J. Immunol. 126:1174–1179.

    Google Scholar 

  21. Veerhuis, R., N. Klar-Mohamad, L.A. Van Es, andM. R. Daha. 1986. Enhanced binding and degradation of the Clq subcomponent of complement by thioglycollate-stimulated guinea pig peritoneal macrophages.Scand. J. Immunol. 23:581–587.

    Google Scholar 

  22. Bing, D. H., S. Almeda, H. Isliker, J. Lahav, andR. O. Hynes. 1982. Fibronectin binds to the Clq component of complement.Proc. Natl. Acad. Sci U.S.A. 79:4198–4201.

    Google Scholar 

  23. Pearlstein, E., J. Sorvillo, andI. Gigli. 1982. The interaction of human plasma fibronectin with a subunit of the first component of complement, Clq.J. Immunol. 128:2036–2039.

    Google Scholar 

  24. Tenner, A. J., andN. R. Cooper. 1982. Stimulation of a human polymorphonuclear leucocyte oxidative response by the Clq subunit of the first complement component.J. Immunol. 128:2547–2552.

    Google Scholar 

  25. Niven, I. P., andK. Whaley. 1986. Inhibition of Clq binding to antigen-antibody complexes by a factor in rheumatoid arthritis serum.Rheumatol. Int. 6:205–208.

    Google Scholar 

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Authors and Affiliations

  1. Research Service, Veterans Administration Medical Center (VAMC), Albany, New York

    Carmen L. Rosano, Nourollah Parhami & Charles Hurwitz

  2. Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York

    Karim E. Hechemy

Authors
  1. Carmen L. Rosano
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  2. Nourollah Parhami
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  3. Karim E. Hechemy
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  4. Charles Hurwitz
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Rosano, C.L., Parhami, N., Hechemy, K.E. et al. Serum levels of RHP and of unbound C1q in rheumatoid arthritis and systemic lupus erythematosus. Inflammation 12, 351–360 (1988). https://doi.org/10.1007/BF00915770

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