Skip to main content
SpringerLink
Log in

Bacteriolytic activity of human gingival exudate

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

We investigated the bacteriolytic activity of gingival crevicular fluid (CF) on14C-labeledStreptococcus faecalis, Streptococcus mutans, Staphylococcus aureus, and on whole dental plaque. CF was collected from 100 healthy donors pooled and centrifuged at 200g. CF supernate and a frozen and thawed extract of the pellet were interacted with the different bacterial strains, whileStreptococcus faecalis andStaphylococcus aureus released 60% and 75% of the radioactive label, only 38% of it was solubilized fromStreptococcus mutans, following their incubation with the CF supernate. The findings agreed with results obtained by interacting bacteria with a frozen and thawed lysate of human peripheral blood leukocytes. On the other hand, extracts from frozen and thawed CF pellet were inactive. Further, lipoteichoic acid and lipopolysaccharide were released by CF from Gram-positive and Gram-negative bacteria, respectively. The role of bateriolytic factors, present in CF, as a result of the interaction between microorganisms and leukocytes at inflammatory sites is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cimasoni, G. 1974. The crevicular fluid.In Monographs in Oral Science, Vol. 3. H. M. Meyers, editor. S. Karger, Basel. 85, 94, 95.

    Google Scholar 

  2. Skapski, H., andT. Lehner. 1976. A crevicular washing method for investigation of immune components of crevicular fluid in man.J. Periodontol. 11:19–24.

    Google Scholar 

  3. Attstrom, R. 1970. Presence of leukocytes in crevices of healthy and chronically inflamed gingivae.J. Periodont. Res. 5:42–47.

    Google Scholar 

  4. Cimasoni, G. 1977. Enzyme activity in the gingival crevice.In The borderline between Caried and Periodontal Disease. T. Lehner, editor. Academic Press, London. 13–41.

    Google Scholar 

  5. Tempel, T. R., R. Snyderman, H. V. Jordan, andS. E. Mergenhaoen. 1971. Factors from saliva and oral bacteria, chemotactic for polymorphonuclear leukocytes: Their possible role in gingival inflammation.J. Periodontol. 41:3–12.

    Google Scholar 

  6. Taichman, N. S., W. P. McArthur, andP. Baehni. 1976. Release of lysosomal enzymes from polymorphonuclear leukocytes (PMN) exposed to whole plaque.J. Dent. Res. 55:213B.

    Google Scholar 

  7. Page, R. C., P. Davies, andA. C. Allison. 1973. Effects of dental plaque on the production and release of lysosomal hydrolases by macrophages in culture.Arch. Oral Biol. 18:1481–1495.

    Google Scholar 

  8. Lahav, M., N. Neeman, E. Adler, andI. Ginsburg. 1974. The effect of leukocyte hydrolases on bacteria. I. Degradation of14C labeled streptococci and staphylococci by leukocyte lysates in vitro.J. Infect. Dis. 129:528–537.

    Google Scholar 

  9. Neeman, N., M. Lahav, andI. Ginsburg. 1974. The effect of leukocyte hydrolases on bacteria. II. The synergistic action of lysozyme and extracts of PMN, macrophages, lymphocytes and platelets in bacteriolysis.Proc. Soc. Biol. Med. 146:1137–1145.

    Google Scholar 

  10. Ehrlich, J., M. N. Sela, M. Lahav, andI. Ginsburg. 1977. The bacteriolytic effect of human dentoalveolar purulent exudates and leukocyte extracts.Isr. J. Dent. Med. 26:39–44.

    Google Scholar 

  11. Sela, M. N., M. Lahav, andI. Ginsburg. 1977. Effect of leukocyte hydrolases on bacteria. IX. The release of lipoteichoic acid from group A streptococci and fromStrep, mutans by leukocyte extracts and by lysozyme. Relation to tissue damage in inflammatory site.Inflammation 2:151–164.

    Google Scholar 

  12. Ferne, M., Z. Duchan, S. Rabinowitz, M. N. Sela, andI. Ginsburg. 1978. The effect of leukocyte hydrolases on bacteria. XII. The release of lipopolysaccharide (LPS) fromSalmonella typhi by leukocyte extracts, lysozyme, inflammatory exudates and by serum and synovial fluid, and the mobulation by anionic and cationic polyelectrolytes of LPS release and the sensitization of erythrocytes.Inflammation 3:59–80.

    Google Scholar 

  13. Ginsburg, I., andM. N. Sela. 1976. The role of leukocytes and their hydrolases in the persistence, degradation and transport of bacterial constituents in tissue: Relation to chronic inflammatory processes in staphylococcal, streptococcal and mycobacterial infections and in chronic periodontal disease.Crit. Rev. Microbiol. 4:249–332.

    Google Scholar 

  14. Ne'eman, N., andI. Ginsburg. 1972. Red cell sensitizing antigen of group A streptococci. II. Immunological and immunopathological properties.Isr. J. Med. Sci. 8:1807–1816.

    Google Scholar 

  15. Fiedel, B. A., and R. W.Jackson. 1974. Implications of a streptococcal lipoteichoic acid in renal dysfunction in hyperimmunized rabbits.Abstract, Annual Meeting American Society of Microbiology. 126.

  16. Wicken, A. J., andK. W. Knox. 1977. Biological properties of lipoteichoic acids.In Microbiology. D. Schlesssinger, editor. A.S.M. Washington, D.C. 360–365.

    Google Scholar 

  17. Gatt, R., andE. R. Berman. 1966. A rapid procedure for the estimation of amino sugars as a micro scale.Anal. Biochem. 15:167–171.

    Google Scholar 

  18. Lowry, O. H., N. J. Rosenbrough, A. L. Farr, andJ. Randall. 1951. Protein measurements with the folin phenol reagent.J. Biol. Chem. 193:265–275.

    Google Scholar 

  19. Loe, H. 1967. The gingival index, the plaque index, and the retention index systems.J. Periodontol. 36:610–616.

    Google Scholar 

  20. Moskowitz, M. 1966. Separation and properties of a red cell sensitizing substance from streptococci.J. Bacteriol. 91:2200–2204.

    Google Scholar 

  21. Brandtzaeg, andW. V. Mann. 1964. A comparative study of the lysozyme activity of human gingival pocket fluid, serum and saliva.Acta. Odontol. Scand. 22:441–455.

    Google Scholar 

  22. Dord, C. E., T. Modeer, P. O. Soder, andJ. Bergstrom. 1971. Enzyme activities in experimental gingivitis in man.Scand. J. Dent. Res. 79:510–514.

    Google Scholar 

  23. Van Palenstein Heldermann, W. H. 1976. Lysozyme concentrations in the gingival crevice and at other oral sites in human subjects with and without gingivitis.Arch. Oral Biol. 21:251–255.

    Google Scholar 

  24. Hausmann, E., D. Luderitz, K.W. Knox, andN. Weinfeld. 1975. Structural requirements for bone resorption by endotoxin and lipoteichoic acid.J. Dent. Res. 54:B94-B99.

    Google Scholar 

  25. Dishon, T., R. Finkel, Z. Marcus, andI. Ginsburg. 1967. Cell sensitizing products of streptococci.Immunology 13:555.

    Google Scholar 

  26. Sela, M. N., A. A. Garfunkel, I. Ginsburg, andT. Dishon. 1978. Modulation of human lymphocyte transformation by Gram-positive and Gram-negative bacterial antigens. 12th International Leukocyte Culture Conference, Beer Sheva, No. 260, 66.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral Biology, The Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel

    M. N. Sela, G. Natan, M. Lahav, I. Ginsburg & T. Dishon

Authors
  1. M. N. Sela
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Natan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Lahav
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Ginsburg
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Dishon
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work forms a part of the DMD thesis of Gad Natan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sela, M.N., Natan, G., Lahav, M. et al. Bacteriolytic activity of human gingival exudate. Inflammation 4, 195–203 (1980). https://doi.org/10.1007/BF00914165

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00914165

Keywords

Search

Navigation