Skip to main content
SpringerLink
Log in

Studies of the effect of auranofin, a new antiarthritic agent, on platelet aggregation

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Auranofin (AF), at a concentration of 10Μg/ml, was found to be a potent inhibitor of ADP-, epinephrine-, or collagen-induced platelet aggregation utilizing platelet-rich plasma obtained from human blood. In contrast, aurothioglucose was less effective than AF in inhibiting epinephrine- or collagen-induced platelet aggregation. The inhibitory effect of AF was more evident on the second phase of aggregation and was a function of drug preincubation time. Compared to platelet-rich plasma, washed platelets were superior for detecting the inhibitory action of AF (⩾0.1 Μg/ml) on ADP-induced platelet aggregation. This potent inhibitory action of AF on ADP-induced platelet aggregation was antagonized by dithioerythriol, a potent reducing agent. These results suggest that AF can inhibit both platelet release and aggregation mechanisms which may be relevant to its antiarthritic activity. Further studies are required to elucidate the cellular mechanism by which AF inhibits platelet aggregation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Valenti, G., U. Chianese, G. Tirri, andM. Giordono. 1979. Thrombocytosis as an inflammation index in rheumatic and connective tissue diseases.In Advances in Inflammation Research Vol. G. Weissman, B. Samuelsson, and R. Paoletti, editors. Raven Press, New York. 579.

    Google Scholar 

  2. Jorgenson, L., T. Horry, H. C. Korsell, andJ. F. Mustard. 1970. Adenosine diphosphate induced platelet aggregation and vascular injury in rabbits.Am. J. Pathol 61:161.

    Google Scholar 

  3. Castor, C. W., J. C. Ritchie, M. E. Scott, andS. L. Whitney. 1977. Connective tissue activation on XL. Simulation of glycosaminoglycogen and DNA formulation by platelet factor.Arthritis Rheum. 20:859.

    Google Scholar 

  4. Finkelstein, A. E., F. R. Roisman, andD. T. Walz. 1977. Effect of Auranofin, a new antiarthritic agent, on immune complex-induced release of lysosomal enzymes from human leukocytes.Inflammation 2:143.

    Google Scholar 

  5. Bull, B. A., M. A. Schneiderman, andG. Breches. 1965. Platelet counts with the Coulter counter.Am. J. Clin. Pathol. 44:678.

    Google Scholar 

  6. Biran, H., A. Dvilansky, I. Nathan, andA. Livne. 1973. Impairment of human platelet aggregation and serotonin release caused in vitro byEchis Colorata venom.Thromb. Diath. Haemorrh. 30:191.

    Google Scholar 

  7. Mustard, J. F., D. W. Perry, N. G. Ardlie, andM. A. Packham. 1972. Preparation of suspensions of washed platelets from humans.Br. J. Haematol. 22:193.

    Google Scholar 

  8. Holmsen, H., H. J. Day, andH. Stormorken. 1969. The blood platelet release reaction.Scand. J. Hematol. Suppl. 8:3.

    Google Scholar 

  9. Weiss, H. J. 1975. Platelet physiology and abnormalities of platelet function.N. Engl. J. Med. 293:531.

    Google Scholar 

  10. Flnkelstein, A. E., F. R. Roisman, V. Batista, F. G. DeNudelman, E. H. DeTitto, M. Mizraji, andD. T. Walz. 1980. Oral chrysotherapy in rheumatoid arthritis: Minimum effective dose.J. Rheum. 7:160–168.

    Google Scholar 

  11. Scheinberg, M. A., L. Santos, andA. E. Finkelstein. 1981. Gold inhibition of human monocyte function.Arthritis Rheum. 24:S117 (abstract).

    Google Scholar 

  12. Salzman, E. W. 1972. Cyclic AMP and platelet function.N. Engl. J. Med. 286:358.

    Google Scholar 

  13. Lamprecht, S., A. E.Finkelstein, D. T.Walz, and J.Kraviec. 1981. Inhibitory effect of Auranofin on PGE1-stimulation of ovarian cyclic AMP formation (in preparation).

  14. Gerrard, J. M., andJ. G. White. 1978. Prostaglandins and thromboxanes: “Middle man” modulating platelet function in hemostasis and thrombosis.Prog. Hemostasis Thromb. 4:87.

    Google Scholar 

  15. Mustard, J. F., andM. Packham. 1970. Factors influencing platelet function: Adhesion release and aggregation.Pharmacol. Rev. 22:97–187.

    Google Scholar 

  16. Finkelstein, A. E., O. R. Burrone, D. T. Walz, andA. Misher. 1977. Effect of Auranofin on DNA and protein synthesis in human lymphocytes.J. Rheumatol. 4:245.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Hematology Department of the Soroka Medical Center, Ben-Gurion University, Beer-Sheba, Israel

    I. Nathan, A. E. Finkelstein, D. T. Walz & A. Dvilansky

  2. The Rheumatology Immunology Department, Fundación CIMAE, Buenos Aires, Argentina

    I. Nathan, A. E. Finkelstein, D. T. Walz & A. Dvilansky

  3. Smith Kline & French Laboratories, Philadelphia, Pennsylvania

    I. Nathan, A. E. Finkelstein, D. T. Walz & A. Dvilansky

Authors
  1. I. Nathan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. E. Finkelstein
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. T. Walz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Dvilansky
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nathan, I., Finkelstein, A.E., Walz, D.T. et al. Studies of the effect of auranofin, a new antiarthritic agent, on platelet aggregation. Inflammation 6, 79–85 (1982). https://doi.org/10.1007/BF00910721

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00910721

Keywords

Search

Navigation