Abstract
The effect of selected compounds with known immunoregulatory activity was examined in a 45-h sensitization period oxazolone contact-sensitivity reaction. Oxazolone sensitivity was induced by applying 0.1 ml of 5% oxazolone in absolute ethanol to the shaved abdomen of C57B1/6 mice on day 0. Challenge with oxazolone followed 45 h later and was accomplished by painting a 5% solution of oxazolone in absolute ethanol on the left hindpaw. The response at 24 h was determined plethysmographically. Histamine (0.062–1.0 mg/kg, subcutaneously, twice a day), concanavalin A (0.31–5.0 mg/kg, intravenously), penicillamine (6.25–25 mg/kg, subcutaneously), chloroquine (6.25– 25 mg/kg, subcutaneously), and thymosin fraction 5 (0.125–1.25 mg/kg subcutaneously) all stimulated the oxazolone reaction when administered on day 0. These data suggest that the low-grade oxazolone response may be a useful assay to detect immunostimulatory activity.
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Asherton, G. L., andW. Ptak. 1968. Contact hypersensitivity in the mouse: I. Active sensitization and passive transfer.Immunology 15:405.
Davies, A. J. S., R. L. Carter, E. Leuchars, andV. Wallis. 1969. The morphology of immune reactions in normal, thymectomized and reconstituted mice: II. The response to oxazolone.Immunology 17:111.
Dietrich, F. M., andR. Hess. 1970. Hypersensitivity inmice: I. Induction of contact sensitivity to oxazolone and inhibition by various chemical compounds.Int. Arch Allergy 38:246.
Pritchard, H., andH. S. Michlen. 1972. Immune response in congenitally thymusless mice: I. Absence of response to oxazolone.Clin. Exp. Immunol. 10:151.
Asherson, G. L., andM. Zembala. 1976. Suppressor T-cells in cell-mediated immunity.Br. Med. Bull 32:158.
Askenase, P. W., andB. J. Hayden. 1974. Cytophilic antibodies in mice contact-sensitized with oxazolone: Immunochemical characterization and preferential binding to a trypsinsensitive macrophage receptor.Immunology 27:563.
Zembala, M., andG. L. Asherson. 1970. Contact sensitivity in the mouse. V. Role of macrophage cytophilic antibody in passive transfer and the effect of trypsin and antigamma globulin serum.Cell. Immunol. 1:276.
Griswold, D. E., J. A. DiLorenzo, andP. Calabresi. 1974. Quantitation and pharmacological dissection of oxazolone-induced contact sensitivity in the mouse.Cell Immunol 11:198.
Evans, D. P., M. Hossack, andD. S. Thomson. 1971. Inhibition of contact sensitivity in the mouse by topical application of corticosteroids.Br. J. Pharmacol. 43:403.
De Sousa, M., andJ. Fachet. 1972. The cellular basis of the mechanism of action of cortisone acetate on contact sensitivity to oxazolone in the mouse.Clin. Exp. Immunol. 10:673.
Griswold, D. E., andD. T. Walz. 1977. Condition-dependent immunoregulatory control of contact sensitivity by levamisole.Inflammation 2:277.
Walz, D. T., andD. E. Griswold. 1978. Immunopharmacology of gold sodium thiomalate and auranofin (SK & F D-39162): Effects on cell-mediated immunity.Inflammation 3:117.
Walz, D. T., M. J. DiMartino, J. K. Kuch, andW. Zuccarello. 1970. Adjuvant-induced arthritis in rats: I. Temporal relationship of physiological, biochemical and hematological parameters.Proc. Soc. Biol. Med. 136:907.
Bach, J. F. 1979. Thymic hormones.J. Immunopharmacol. 1:277–310.
Low, T. L., G. B. Thurman, C. Chincarini, J. E. McClune, G. D. Marshall, S. K. Hu, andA. L. Goldstein. 1979. Current status of thymosin research: evidence for the existence of a family of thymic factors that control T-cell maturation.Ann. N. Y. Acad. Sci. 332:33–48.
Talal, N., M. Dauphinee, M. Pillarisetty, andR. Goldblum. 1975. Effect of thymosin on thymocyte proliferation and autoimmunity in NZB mice.Ann. N. Y. Acad Sci. 249: 438–561.
Flaut, M., L. M. Lichtenstein, E. Gillespie, andC. S. Henney. 1973. Studies on the mechanism of lymphocyte-mediated cytolysis. IV. Specificity of the histamine receptor on effector T cells.J. Immunol. 111:389–394.
Schwartz, A., P. W. Askenase, andR. K. Gershon. 1980. Histamine Inhibition of the in vitro induction of cytotoxic T-cell response.Immunopharmacology 2:179–190.
Shearer, G. M., K. L. Melmon, Y. Weinstein, andM. Sela. 1972. Regulation of antibody response by cells expressing histamine receptors.J. Exp. Med. 136:1302–1307.
Rocklin, R. E., S. Greineder, andB. H. Littman. 1978. Modulating cellular immune function in vitro by histamine-bearing lymphocytes: Mechanism of action.Cell. Immunol. 37:162–173.
Schwartz, A., S. L. Sutton, P. W. Askenase, andR. K. Gershon. 1981. Histamine inhibition of concanavalin A-induced suppressor T-cell activation.Cell. Immunol. 60:426–439.
Griswold, D. E., D. T.Walz, and S.Alessi. 1981. Stimulation of contact sensitivity by disodium cromoglycate.Int. Archs. Allergy Appl. Immun. (in press).
Andersson, J., andF. Melchers. 1976. Lymphocyte stimulation by Concanavalin A.In Concanavalin A as a Tool. H. Bittiger and H. P. Schnebli, editors. Wiley & Sons, New York. 505.
Keller, R. 1976. Concanavalin A as a probe for the in vitro detection of IgE-type antibody on the surface of blood basophils and tissue mast cells.In Concanavalin A as a Tool. H. Bittiger and H. P. Schnebli, editors. Wiley & Sons, New York. 581.
Jaffe, J. H., andW. R. Martin. 1975. Narcotic analgesics and antagonists.In The Pharmacological Basis for Therapeutics. L. S. Goodman and A. Gilman, editors. Macmillan, New York. 245–283.
Finkelstein, A. E., D. T. Walz, V. Batista, M. Mizrazi, F. Roisman, andA. Misher. 1976. Auranofin, a new oral gold compound for treatment of rheumatoid arthritis.Ann. Rheum. Dis. 35:251–257.
Zvaifler, N. J. 1979. Gold and antimalarial therapy.In Arthritis and Allied Conditions, D. J. McCarty, editor. Lea & Febiger, Philadelphia. 355–367.
Jaffe, I. A., andW. R. Martin. 1975. Narcotic analgesics and antagonists.In The Pharmacological Basis for Therapeutics. L. S. Goodman and A. Gilman, editors. Macmillan, New York. 245–283.
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Griswold, D.E., Walz, D.T. Effect of selected immunoregulatory agents on low-grade contact sensitivity. Inflammation 6, 55–62 (1982). https://doi.org/10.1007/BF00910719
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DOI: https://doi.org/10.1007/BF00910719