Summary
The frequency of ultraviolet-induced adenine reversions relative to inositol reversions in the strainK3/17 ad-3A 38701: inos 37401 is influenced by temperature. Fewer adenine revertants are obtained from a given ultraviolet dose adminstered at 30°C than from the same dose administered at 2°C. The inositol reversion frequency and the percentage survival are not greatly affected by these differences in temperature. Temperature differences are only effective if a) applied during irradiation b) higher temperatures above 15°C are used. Possible explanations for these results are considered.
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Literature
Beadle, G. W., andE. L. Tatum: Methods of producing and detecting mutations concerned with nutritional requirements. Amer. J. Bot.32, 678–686 (1945).
Bowne, S. W., andP. Rogers: Ultraviolet light and enzyme synthesis. J. molec. Biol.5, 90–96 (1962).
Doudney, C. O., andF. L. Haas: Mutation induction and macromolecular synthesis in bacteria.Proc. nat. Acad. Sci. (Wash.)45, 709–722 (1959).
——: Some biochemical aspects of the postirradiation modification of ultraviolet-induced mutation frequency in bacteria. Genetics45, 1481–1502 (1960).
Kølmark, H. G., andB. J. Kilbey: An investigation into the mutagenic after-effect of Butadiene diepoxide usingNeurospora crassa. Z. Vererb.-Lehre93, 356–365 (1962).
Setlow, R. B., andJ. K. Setlow: Evidence that ultraviolet induced thymine dimers cause biological damage. Proc. nat. Acad. Sci. (Wash.)48, 1250 (1962)_.
Witkin, E. M.: Time, temperature and protein synthesis: A study of ultraviolet-induced mutation in bacteria. Cold Spr. Harb. Symp. quant. Biol.21, 123–140 (1956).
—, andE. C. Theil: The effect of posttreatments with chloramphenicol on various ultraviolet-induced mutations inEscherichia coli. Proc. nat. Acad. Sci. (Wash.)46, 226–232 (1960).
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Kilbey, B.J. The influence of temperature on the ultraviolet induced revertant frequencies of two auxotrophs ofNeurospora crassa . Zeitschrift für Vererbungslehre 94, 385–391 (1963). https://doi.org/10.1007/BF00897595
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DOI: https://doi.org/10.1007/BF00897595