Summary
E. coli KB (Benzer) differs from other common laboratory strains in possessing a glycyl sRNA synthetase with a 50 to 100 times elevated K m for glycine. The degree of charging of glycyl sRNA in this strain can be increased by supplementing the growth medium with glycine. The altered enzyme has been used as a marker by which to map its structural gene. Linkage analysis of recombinants from uninterrupted matings, and cotransduction (80%) of the synthetase withxyl, indicate that this gene is located betweenxyl andmalt, close toxyl, at min 69.5 on the map drawn byTaylor andThoman (1964).
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Literature
Böck, A., L. E. Faiman, andF. C. Neidhardt: Biochemical and genetic characterization of a mutant ofEscherichia coli with a temperature-sensitive valyl ribonucleic acid synthetase. J. Bact.92 (in press) (1966).
Eidlic, L., andF. C. Neidhardt: Role of vavyl sRNA synthetase in enzyme repression. Proc, nat. Acad. Sci. (Wash.)53, 539–543 (1965a).
——: Protein and nucleic acid synthesis in two mutants ofEscherichia coli with temperature-sensitive aminoacyl ribonucleic acid synthetases. J. Bact.89, 706–711 (1965b).
Fangman, W. L., G. Nass, andF. C. Neidhardt: Immunological and chemical studies of phenylalanyl sRNA synthetase inEscherichia coli. J. molec. Biol.13, 202–219 (1965).
—, andF. C. Neidhardt: Demonstration of an altered aminoacyl ribonucleic acid synthetase in a mutant ofEscherichia coli. J. biol. Chem.239, 1839–1843 (1964).
Gorini, L., andH. Kaufman: Selecting bacterial mutants by the penicillin method. Science131, 604–605 (1960).
Lederberg, J., L. L. Cavalli, andE. M. Lederberg: Sex compatibility inEscherichia coli. Genetics37, 720–730 (1952).
Lennox, E. S.: Transduction of linked genetic characters of the host by bacteriophage Pl. Virology1, 190–206 (1955).
Morris, D. W., andJ. A. De Moss: Role of aminoacyl transfer ribonucleic acid in the regulation of ribonucleic acid synthesis inEscherichia coli. J. Bact.90, 1624–1631 (1965).
Nass, G., andF. C. Neidhardt: Histidinyl sRNA synthetase and enzyme repression inEscherichia coli. Bact. Proc. 87 (1966).
Roth, J. R., D. Gilbert, G. Fink, M. J. Voll, P. E. Hartman, andB. Ames: Transfer RNA and the control of the histidine operon. Cold Spr. Harb. Symp. quant. Biol.31 (in press) (1966).
Schlesinger, S., andB. Magansaik: Effect of α-methylhistidine on the control of histidine synthesis. J. molec. Biol.9, 670–682 (1964).
Taylor, A. I., andM. S. Thoman: The genetic map ofEscherichia coli K12. Genetics50, 659–677 (1964).
Yaniv, M., andF. Gros: Studies on temperature conditional mutant ofEscherichia coli with a modified aminoacyl RNA synthetase. Proc. 3rd. Fed. Europ. Biochem. Soc., Warsaw, April 1966.
—F. Jacob andF. Gros: Mutations thermosensibles des systems activant la valine chezE. coli. Bull. Soc. Chim. biol. (Paris)47, 1609–1656 (1965).
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Böck, A., Neidhardt, F.C. Location of the structural gene for glycyl ribonucleic acid synthetase by means of a strain ofEscherichia coli possessing an unusual enzyme. Zeitschrift für Vererbungslehre 98, 187–192 (1966). https://doi.org/10.1007/BF00888946
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DOI: https://doi.org/10.1007/BF00888946