Summary
The effects of extracellular UV-irradiation on the replication of DNA were tested with phage T2. Cells ofE. coli B/1 were multiply infected with UV-irradiated T2. The kinetics of P32-incorporation into phage DNA were significantly different from the unirradiated control.
With unirradiated phages the time curve is linear during the second half of the latent period after as short nonlinear increase. With UV-irradiated phages however the amount of DNA increases exponentially during the whole latent period. Such kinetics might be expected from semiconservative DNA-replication if templates were limiting. The reported findings suggest that other regulatory mechanisms normally limiting DNA-synthesis are inactivated by UV. The kinetics determined by semiconservative replication could then be clearly observed with irradiated phages. The nature of the normally regulating mechanisms is discussed.
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Kölsch, E. Der Einfluss extrazellulärer UV-Bestrahlung des Phagen T2 auf die Replikation Seiner DNA. Zeitschrift für Vererbungslehre 96, 168–174 (1965). https://doi.org/10.1007/BF00888786
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DOI: https://doi.org/10.1007/BF00888786