Summary
In vivo labelling of RNA polymerases I and II with S35-methionine shows that ecdysterone causes a de novo synthesis of both enzymes in nuclei of fat body cells inCalliphora 3rd instar larvae. Binding experiments with H3-α-amanitin demonstrate that the concentration of RNA polymerase II molecules increases two- to threefold within 3 h after ecdysterone treatment. The enhanced polymerase concentration coincides with an enhanced enzymatic activity and an increase in RNA synthesis in response to ecdysteroids.
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Schenkel, H., Scheller, K. Ecdysteroids regulate the synthesis of RNA polymerase molecules in fat body cells ofCalliphora larvae. Wilhelm Roux' Archiv 192, 152–158 (1983). https://doi.org/10.1007/BF00848684
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DOI: https://doi.org/10.1007/BF00848684