Abstract
The mouse liver was fixed in 12 different kinds of fixative based on glutaraldehyde, paraform-aldehyde, picric acid, and lysine and the location of albumin was studied by an indirect immunoperoxidase method. Comparison of the results showed that the best method of fixation is by immersion in 8% paraformaldehyde solution. Albumin was found on ribosomes and nuclear membranes and on the granular endoplasmic reticulum and also in the matrix of the hepatocyte cytoplasm. It is suggested that newly synthesized albumin is secreted directly into the cytosol, from which it enters the extracellular space.
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Baranov, V.N., Rukosuev, V.S. An indirect immunoelectron-microscopic method of locating albumin in mouse hepatocytes. Bull Exp Biol Med 88, 1211–1214 (1979). https://doi.org/10.1007/BF00838211
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DOI: https://doi.org/10.1007/BF00838211