Abstract
Electron microscopy samples processed for cryo-immunogold-labeling need to be gently fixed to keep their antigenicity. Biological material like cultured cells or tissue can be prepared according to the standard Tokuyasu fixation or in a further developed rehydration method based on high-pressure freezing. We will describe here the variant and common steps of both methods in detail and illustrate their potency in the ultrastructural imaging of peroxisomes.
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Acknowledgments
We thank Gerd Hause, University of Halle/S., for providing the instruction and material for self-made baskets.
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Mildner, K., Zeuschner, D. (2017). Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins. In: Schrader, M. (eds) Peroxisomes. Methods in Molecular Biology, vol 1595. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6937-1_11
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DOI: https://doi.org/10.1007/978-1-4939-6937-1_11
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6937-1
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