Abstract
A naturally occurring antiglobulin factor against the Fab-fragment of homologous IgG, contained in the rabbit and human blood serum and γ-globulin preparations, was shown by gelfiltration on Sephadex G-200 to have a molecular weight of about 250,000 daltons. In man this factor does not pass through the placenta in normal pregnancy. Despite differences in the physicochemical and effector properties of 7S IgG and protein with homoreactant activity, the latter has the specific antigenic determinants of IgG. These observations suggest that a complex of IgG with another protein or nonprotein compound possesses homoreactant properties.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature Cited
A. Ya. Kul'berg and N. E. Shmeleva, “The immunology of homoreactants,” Zh. Mikrobiol., No. 7, 97 (1974).
A. Ya. Kul'berg, N. E. Shmeleva, L. M. Bartova, et al., “Investigation of the biological activity of pepsin Fab-fragments of homologous and autologous G-globulin,” Byull. Éksperim. Biol. i Med., No. 7, 64 (1971).
W. Baxter, W. J. Mandy, and A. Malley, “Natural antiglobulin antibodies in primate sera,” Fed. Proc.,28, 3130 (1969).
J. C. Cerotini, “An antigen-binding capacity test for human immunoglobulin G (IgG) fragments,” J. Immunol.,101, 433 (1968).
J. L. Fahey and E. M. Keley, “Quantitative determinations of serum immunoglobulins in antibody agar plates,” J. Immunol.,94, 84 (1965).
H. H. Fudenberg, “Compleat immunology: science or septophrenia?” Clin. Exp. Immunol.,2, 1 (1967).
F. C. Hay, M. G. R. Hull, and G. Torringiani, “The transfer of human IgG subclasses from mother to fetus,” Clin. Exp. Immunol.,9, 355 (1971).
M. Harboe, B. Rau, and K. Aho, “Properties of various anti-gamma-globulin factors in human sera,” J. Exp. Med.,121, 503 (1965).
L. C. Kormeir, J. T. Ing, and W. J. Mandy, “Specificity of antiglobulin factors in normal human serum reacting with enzyme-digested IgG globulin,” J. Immunol.,100, 612 (1968).
A. Ya. Kul'berg (A. J. Kulberg), L. M. Bartova, and L. M. Shmeleva (L. M. Shmelieva), “Interaction of anti-DNP rabbit antibody with peptic Fab'-fragment of homologous IgG,” Immunochemistry,8, 733 (1971).
A. Ya. Kul'berg (A. J. Kulberg), “Immunoglobulin effector functions. Molecular mechanisms,” Allerg. Immunol.,18, 310 (1972).
W. J. Mandy, H. H. Fudenberg, and F. J. Lewis, “A new serum factor in normal rabbits. 1. Identification and characterization,” J. Immunol.,85, 501 (1965).
O. J. Mellbye and J. B. Natvig, “Evidence for immune complexes containing antibody to the pepsin of IgG in rheumatoid synovial fluids,” Clin. Exp. Immunol.,8, 889 (1971).
C. K. Osterland, M. Harboe, and H. G. Kunkel, “Anti-gamma-globulin factors in human sera revealed by enzymatic splitting of anti-Rh antibodies,” Vox Sang. (Basel),8, 133 (1963).
A. J. Rawson, F. P. Qusmorio, and N. M. Abelson, “The induction of synovitis in the normal rabbit with Fab. A possible experimental model of rheumatoid arthritis,” Arthr. Rheum.,11, 505 (1968).
E. R. Richie, M. E. Woolsey, and W. J. Mandy, “A new serum factor in normal rabbits. VI. Reaction with buried determinants of IgG exposed sequentially with CNBr, pepsin, and papain,” J. Immunol.,104, 984 (1970).
M. Waller, N. Curry, and A. J. Mallory, “Immunochemical and serological studies of enzymatically fragmented human IgG globulins. II. Hydrolysis with subtilisin, elastase, trypsin, and chymotrypsin,” Immunochemistry,6, 207 (1969).
W. Waller and R. J. Duma, “Increased antibiodies to IgG fragments: correlation with infection due to Gram-positive bacteria,” J. Infect. Dis.,125, 45 (1972).
S. Watanabe and M. Kitagawa, “Naturally occuring rabbit antibodies against the tryptic 3.5S and 5S fragments of rabbit IgG-II,” Immunochemistry,7, 363 (1970).
A. C. Wang, W. P. Faulk, M. A. Stuckey, et al., “Chemical differences of adult, fetal, and hypogammaglobylinemic IgG immunoglobulins,” Immunochemistry,7, 703 (1970).
M. E. Woolsey and W. J. Mandy, “A new serum factor in normal rabbits. Induction in neonatal rabbits,” J. Immunol.,104, 475 (1970).
Rights and permissions
About this article
Cite this article
Kul'berg, A.Y., Bartova, L.M., Reznikov, Y.P. et al. Some immunochemical properties of natural antiglobulin factors (homoreactants). Bull Exp Biol Med 80, 796–798 (1975). https://doi.org/10.1007/BF00809894
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00809894