Summary
DNA sequence analysis of the virus TTV1 identified in archaebacteriumThermoproteus tenax revealed an open reading frame possibly encoding for a protein. To assess the expression of this hypothetical protein F154 via immunochemical methods, related synthetic fragments should allow for production of protein specific antisera. For this purpose the dodecapeptide amide H-Thr-Pro-Thr-Pro-Thr-Pro-Thr-Pro-Ser-Pro-Thr-Pro-NH2 related to a characteristic repeat in the protein primary structure was prepared by conventional methods of peptide synthesis.
Zusammenfassung
Sequenzanalyse des Genoms des Virus TTV1, der im ArchaebakteriumThermoproteus tenax aufgefunden wurde, läßt die Expression eines Proteins zu, dessen tatsächliche Existenz aber nicht gesichert ist. Antiseren gegen synthetische Fragmente dieses hypothetischen Proteins sollten dessen Identifizierung und somit indirekt den Nachweis der Expression im Bakterium ermöglichen. Zu diesem Zweck erfolgte die Synthese des Dodekapeptidamids H-Thr-Pro-Thr-Pro-Thr-Pro-Thr-Pro-Ser-Pro-Thr-Pro-NH2, eines charakteristischen Fragments dieses Proteins, mit Hilfe konventioneller Methoden.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Zillig W., Gropp F., Henschen A., Neumann H., Palm P., Reiter W. D., Rettenberger M., Schnabel H., Yeats S. (1986) System. Appl. Microbiol.7: 58
Janekovic D., Wunderl S., Holz I., Zillig W., Gierl A., Neumann H. (1983) Mol. Gen. Genet.192: 39
For reviews concerning the use of antipeptide antisera see: Lerner R. A. (1982) Nature299: 592; Sutcliff J. G., Shinnick T. M., Green N., Lerner R. A. (1983) Science219: 660; Walter G. (1986) J. Immunol. Meth.88: 149
Wünsch E., Moroder L., Nyfeler R., Kalbacher H., Gemeiner M. (1985) Biol. Chem. Hoppe-Seyler366: 53; Moroder L., Mourier G., Dufresne M., Bovermann G., Göhring W., Gemeiner M., Wünsch E. (1987) Biol. Chem. Hoppe-Seyler368: 839
Krois D., Simonetti M., Wünsch E. (1989) Monatsh. Chem.120: 1029
Krois D., Dufresne M., Neumann H., Moroder L., Wünsch E. (in preparation)
Frank H., Nicholson G. J., Bayer E. (1977) J. Chromatogr. Sci.15: 17
Wünsch E. (ed.) In: Houben-Weyl (1974) Methoden der Organischen Chemie: Synthese von Peptiden, Vol. 15. Thieme, Stuttgart
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Dr. Karl Schlögl on occasion of his 65th birthday
Rights and permissions
About this article
Cite this article
Wünsch, E., Krois, D. On the hypothetical protein F154 of the TTV1 virus/Thermoproteus Tenax. Part I: Synthesis of a dodecapeptide amide related to the protein sequence 73–84. Monatsh Chem 120, 1021–1028 (1989). https://doi.org/10.1007/BF00808774
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00808774